Detailed information of TA system TA022838

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 1304688..1305264
• Replicon: chromosome
• Accession: NC_010628
• Organism: Nostoc punctiforme PCC 73102 (Nostoc punctiforme ATCC 29133)

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: B2IW72
• Locus tag: NPUN_RS05555
• Protein ID: WP_012407838.1
• Coordinates: 1304905..1305264 (+)
• Length: 120 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: B2IW71
• Locus tag: NPUN_RS05550
• Protein ID: WP_012407837.1
• Coordinates: 1304688..1304918 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NPUN_RS05525	1299871..1300233	+	363	WP_012407832.1	response regulator	-
NPUN_RS05530	1300266..1300466	+	201	WP_012407833.1	type II toxin-antitoxin system VapB family antitoxin	-
NPUN_RS41580	1300463..1300636	+	174	WP_167315577.1	hypothetical protein	-
NPUN_RS05535	1300745..1303195	+	2451	WP_012407834.1	phenylalanine--tRNA ligase subunit beta	-
NPUN_RS05540	1303305..1303574	+	270	WP_012407835.1	YciI family protein	-
NPUN_RS05545	1303703..1304497	+	795	WP_012407836.1	Tab2/Atab2 family RNA-binding protein	-
NPUN_RS05550	1304688..1304918	+	231	WP_012407837.1	hypothetical protein	Antitoxin
NPUN_RS05555	1304905..1305264	+	360	WP_012407838.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
NPUN_RS05560	1305596..1306795	-	1200	WP_012407839.1	DUF4159 domain-containing protein	-
NPUN_RS05565	1306869..1307897	-	1029	WP_012407840.1	phage tail protein	-
NPUN_RS05570	1307975..1310173	-	2199	WP_012407841.1	putative baseplate assembly protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (9-115)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 120 a.a.
• Molecular weight: 13350.56 Da
• Isoelectric Point: 8.9313

>T22838 WP_012407838.1 NC_010628:1304905-1305264 [Nostoc punctiforme PCC 73102]
MPGSNLTYRRGEIRWVNLDPTVGAEAKKIRACLIVQNDIMNQYGLLTIVMPFRPGSKQAPYVVNVKATPNNGLDQDRFID
IGQIRAVDHSRILGLLGVLESEYWELIRTALNIVLGFVL

Nucleotide

• Length: 360 bp

>T22838 NC_010628:1304905-1305264 [Nostoc punctiforme PCC 73102]
ATGCCAGGGAGTAATTTAACTTATCGGCGAGGAGAAATACGTTGGGTGAATCTCGATCCAACAGTGGGCGCTGAGGCAAA
AAAAATCCGCGCTTGCTTGATTGTGCAAAATGACATCATGAATCAGTATGGGTTGCTGACAATTGTGATGCCATTTCGGC
CCGGAAGTAAACAAGCGCCCTACGTTGTAAATGTGAAAGCAACACCAAATAATGGACTAGACCAAGACCGTTTTATAGAT
ATTGGCCAAATTCGTGCTGTTGACCATAGTCGTATTTTGGGCTTGCTGGGCGTGCTGGAGTCAGAATACTGGGAACTTAT
TCGTACAGCCTTAAATATAGTTCTGGGTTTTGTGCTTTAG

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8563.62 Da
• Isoelectric Point: 4.1677

>AT22838 WP_012407837.1 NC_010628:1304688-1304918 [Nostoc punctiforme PCC 73102]
MLKVTITLEEDILQFVDQYAQGNRSAYINTLLAEHRRRILAAEMIAALKQDAEDPEYQAEIAAWDSVVGDGINARE

Nucleotide

• Length: 231 bp

>AT22838 NC_010628:1304688-1304918 [Nostoc punctiforme PCC 73102]
ATGCTCAAAGTTACAATTACCTTAGAAGAAGACATTCTGCAATTTGTAGATCAGTATGCACAGGGAAATCGTAGTGCATA
CATCAATACACTGCTAGCAGAACACCGCCGCCGAATTTTGGCAGCAGAAATGATTGCAGCCCTCAAGCAGGATGCTGAAG
ATCCAGAATATCAAGCTGAAATTGCTGCTTGGGATAGTGTTGTTGGGGATGGCATAAATGCCAGGGAGTAA

Structures

Toxin

• AlphaFold DB: B2IW72

Antitoxin

• AlphaFold DB: B2IW71