Detailed information of TA system TA228376

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsaAT/-
• Location: 1201795..1202272
• Replicon: chromosome
• Accession: NZ_CP089586
• Organism: Staphylococcus aureus strain E1185_IV_ST12

Toxin (Protein)

• Gene name: TsaT
• Uniprot ID: T1Y7S2
• Locus tag: LUU82_RS06150
• Protein ID: WP_000052076.1
• Coordinates: 1202084..1202272 (+)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: TsaA
• Uniprot ID: T1Y6P8
• Locus tag: LUU82_RS06145
• Protein ID: WP_001059690.1
• Coordinates: 1201795..1202055 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LUU82_RS06110 (1197188)	1197188..1197904	-	717	WP_000150016.1	type I 3-dehydroquinate dehydratase	-
LUU82_RS06115 (1198052)	1198052..1198474	+	423	WP_000974448.1	organic hydroperoxide resistance protein	-
LUU82_RS06120 (1198873)	1198873..1199367	+	495	WP_000569884.1	GNAT family N-acetyltransferase	-
LUU82_RS06125 (1199522)	1199522..1200139	+	618	WP_000255547.1	LysE/ArgO family amino acid transporter	-
LUU82_RS06130 (1200212)	1200212..1200793	-	582	WP_001140696.1	histidine phosphatase family protein	-
LUU82_RS06135 (1201018)	1201018..1201254	+	237	WP_000368687.1	hypothetical protein	-
LUU82_RS06140 (1201251)	1201251..1201454	-	204	WP_000145163.1	sterile alpha motif-like domain-containing protein	-
LUU82_RS06145 (1201795)	1201795..1202055	+	261	WP_001059690.1	hypothetical protein	Antitoxin
LUU82_RS06150 (1202084)	1202084..1202272	+	189	WP_000052076.1	hypothetical protein	Toxin
LUU82_RS06155 (1202461)	1202461..1203030	+	570	WP_001106651.1	lipoprotein N-acylation protein LnsA	-
LUU82_RS06160 (1203118)	1203118..1203402	+	285	WP_232652344.1	hypothetical protein	-
LUU82_RS06165 (1203464)	1203464..1203682	+	219	WP_000005886.1	hypothetical protein	-
LUU82_RS06170 (1204178)	1204178..1204378	-	201	WP_001059082.1	cold-shock protein	-
LUU82_RS06175 (1204735)	1204735..1205421	-	687	WP_001806958.1	thermonuclease family protein	-
LUU82_RS06180 (1205762)	1205762..1206244	-	483	WP_000824468.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	seb / vWbp	1175097..1219078	43981

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Cro/C1-type helix-turn-helix domain (49-91)

Antitoxin

• Toxin HigB-like (13-98)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 7142.82 Da
• Isoelectric Point: 9.9971

>T228376 WP_000052076.1 NZ_CP089586:1202084-1202272 [Staphylococcus aureus]
MSLHFAILFWLALIFLVAATFILVLMKKTGKESKKESYLSFTVILYIFGFAILIYTFIFGVL

Nucleotide

• Length: 189 bp

>T228376 NZ_CP123897:c1002803-1002489 [Methylomonas sp. UP202]
ATGACAATCAAATTAAAACGCTGGGATTCGGCCGAGCATTTACAAACCGACGAAGACATCGAATTGTATTTGCAAGCTTG
TTTCGAAGAAGCCGGCGACGACGCCGCCTTTATCGCCAAAGCCTTGGGCAATGTCGCCCGCGCCCGCAGCATGACGCAGT
TGGCCAAGGATACCGGTATGGGGCGGGAAAGTTTATATAAAGCGTTGTCCGGCGAGGGTAATCCAAGTTTCGGCACTATT
TTGAAGGTGACAAATGCATTGGGACTGAAATTTAACGTCCATCTTTCGGATGATTCTGATCGGAAAATGGCTTGA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9940.09 Da
• Isoelectric Point: 4.0624

>AT228376 WP_001059690.1 NZ_CP089586:1201795-1202055 [Staphylococcus aureus]
MNNILLNAINIVITTTFVIFNILITYNKDLDDLCWLLPGIIICGVILIVSFTIAMITKNWLSEILFFINIVLVLYYIYPI
FYSFIG

Nucleotide

• Length: 261 bp

>AT228376 NZ_CP123897:c1003108-1002800 [Methylomonas sp. UP202]
GTGAAAACCGTTTTAACGACCGAGCTTTTCGACCACTGGTTTACCGGCTTGAAAGACATTCAGGCCAAACGTCGTATCCA
AATGCGTATCGACCGTGCCGAGGATGGTCATTTCGGCGATTGCAAACCGGTTGGCGAAGGCGTATCCGAAATGCGCGTCA
ACACCGGTCCGGGTTATCGGATTTATTACACGCAACGTGGAGACGGCTACGAAATCGTTGTACTACTGGCTGGCGGCGAT
AAGTCCTCGCAGGCTCAAGACATCAAGACCGCTATTCAGTTGGCCAGACAAATTAAGGAGCAATCATGA

Structures

Toxin

• AlphaFold DB: A0A0E0VP18

Antitoxin

• AlphaFold DB: A0A0E0VN56