Detailed information of TA system TA022837

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-PHD
• Location: 473167..473807
• Replicon: chromosome
• Accession: NC_010628
• Organism: Nostoc punctiforme PCC 73102 (Nostoc punctiforme ATCC 29133)

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: B2J5Y8
• Locus tag: NPUN_RS01910
• Protein ID: WP_012407180.1
• Coordinates: 473409..473807 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: B2J5Y7
• Locus tag: NPUN_RS01905
• Protein ID: WP_012407179.1
• Coordinates: 473167..473412 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NPUN_RS01895	468460..470055	+	1596	WP_012407177.1	nickel-dependent hydrogenase large subunit	-
NPUN_RS01900	470138..472756	-	2619	WP_202947528.1	AAA family ATPase	-
NPUN_RS01905	473167..473412	+	246	WP_012407179.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
NPUN_RS01910	473409..473807	+	399	WP_012407180.1	type II toxin-antitoxin system VapC family toxin	Toxin
NPUN_RS01915	474022..474444	+	423	WP_012407181.1	hypothetical protein	-
NPUN_RS01920	474590..475063	+	474	WP_012407182.1	hydrogenase maturation protease	-
NPUN_RS01925	475083..475457	-	375	WP_012407183.1	hypothetical protein	-
NPUN_RS01930	475476..476762	-	1287	WP_012407184.1	SagB/ThcOx family dehydrogenase	-
NPUN_RS01935	476737..477234	-	498	WP_012407185.1	ankyrin repeat domain-containing protein	-
NPUN_RS01940	477884..478093	+	210	WP_012407186.1	molybdopterin-binding protein	-
NPUN_RS01945	478300..478560	-	261	WP_012407187.1	ferrous iron transport protein A	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• VapC-like Sll0205 protein, PIN domain (4-128)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-43)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.29 Da
• Isoelectric Point: 4.6485

>T22837 WP_012407180.1 NC_010628:473409-473807 [Nostoc punctiforme PCC 73102]
VKLLLDTQCWLWWFTQPELLNESAIAHIADETNELWLSVASIWEMGIKVAIGKLPLADPLDSYISSRMTVLAMRSLEITA
SHALQAAALPLHHRDPFDRMLIAQAQIEEMTLVSADSMFNKYDISLLWAAKS

Nucleotide

• Length: 399 bp

>T22837 NC_010628:473409-473807 [Nostoc punctiforme PCC 73102]
GTGAAACTTTTGCTAGATACACAATGCTGGTTATGGTGGTTTACCCAACCAGAGCTTTTGAATGAATCAGCAATCGCCCA
TATTGCCGATGAAACAAATGAATTGTGGCTCTCAGTTGCCAGCATTTGGGAAATGGGGATAAAAGTTGCGATCGGGAAGT
TGCCTCTGGCAGATCCCCTAGACAGTTATATTTCTAGTCGGATGACGGTATTGGCAATGCGATCGCTAGAAATTACAGCT
TCTCATGCTTTACAAGCGGCTGCGTTACCTTTGCACCACCGAGATCCTTTTGACAGAATGTTGATTGCACAGGCTCAGAT
AGAAGAAATGACGCTTGTGAGTGCCGATTCAATGTTTAATAAGTACGACATTTCCCTACTTTGGGCAGCCAAATCTTAA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 8918.14 Da
• Isoelectric Point: 4.7661

>AT22837 WP_012407179.1 NC_010628:473167-473412 [Nostoc punctiforme PCC 73102]
METVNIHQAKTNLSKLLSRVELGEEIIISNRGVPIAKLVPFRTSSNRLNSLGQDKGRFVVPDDFNAPLPEEILAAFEGGE
E

Nucleotide

• Length: 246 bp

>AT22837 NC_010628:473167-473412 [Nostoc punctiforme PCC 73102]
ATGGAAACTGTAAATATTCATCAAGCTAAAACGAATCTCTCAAAGCTGTTGTCACGCGTAGAACTTGGAGAAGAAATCAT
TATTTCCAACCGAGGCGTTCCGATCGCCAAGTTGGTTCCGTTTCGCACCTCATCAAATCGACTCAATAGTTTAGGGCAAG
ATAAAGGGCGTTTTGTAGTGCCAGATGACTTCAACGCGCCTTTACCAGAAGAAATTTTGGCAGCATTTGAGGGCGGTGAG
GAGTGA

Structures

Toxin

• AlphaFold DB: B2J5Y8

Antitoxin

• AlphaFold DB: B2J5Y7