Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tad-ata/COG5606-COG4679
Location 22219..22912 Replicon plasmid pPVG21
Accession NZ_CP089533
Organism Pseudomonas veronii strain G2

Toxin (Protein)


Gene name tad Uniprot ID N2IHR9
Locus tag LUW10_RS31450 Protein ID WP_003151133.1
Coordinates 22219..22596 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name ata Uniprot ID N2IIN5
Locus tag LUW10_RS31455 Protein ID WP_001172026.1
Coordinates 22577..22912 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LUW10_RS31435 (LUW10_31435) 17537..18136 - 600 WP_232591925.1 type IV secretion system protein VirB10 -
LUW10_RS31440 (LUW10_31440) 18412..21441 - 3030 WP_010799689.1 Tn3 family transposase -
LUW10_RS31445 (LUW10_31445) 21425..22027 - 603 WP_010465829.1 recombinase family protein -
LUW10_RS31450 (LUW10_31450) 22219..22596 + 378 WP_003151133.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
LUW10_RS31455 (LUW10_31455) 22577..22912 + 336 WP_001172026.1 helix-turn-helix transcriptional regulator Antitoxin
LUW10_RS31460 (LUW10_31460) 22927..23262 + 336 WP_000741275.1 hypothetical protein -
LUW10_RS31465 (LUW10_31465) 23286..23612 + 327 WP_000091614.1 hypothetical protein -
LUW10_RS31470 (LUW10_31470) 23609..23980 + 372 WP_023294894.1 hypothetical protein -
LUW10_RS31475 (LUW10_31475) 23949..24353 - 405 WP_232591928.1 hypothetical protein -
LUW10_RS31480 (LUW10_31480) 24388..24924 - 537 WP_016969807.1 hypothetical protein -
LUW10_RS31485 (LUW10_31485) 24937..25524 - 588 WP_011154390.1 hypothetical protein -
LUW10_RS31490 (LUW10_31490) 25748..26638 + 891 WP_016969805.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..155798 155798


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(16-109)

Antitoxin

Cro/C1-type helix-turn-helix domain

(40-93)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 13705.78 Da        Isoelectric Point: 9.4693

>T228361 WP_003151133.1 NZ_CP089533:22219-22596 [Pseudomonas veronii]
MTNKEKPLEWIASSHKDLMALPSDVRRRFGYALSLAQIGDQDDAAKVLKGFGGAGVLEVVEDDAGGTYRAVYTVKFAEAV
FVLHCFQKKSKSGIATPKADMDIIRARLKVAEVLAQELRNAKTNH

Download         Length: 378 bp

>T228361 NZ_CP123852:2362752-2363018 [Staphylococcus aureus]
ATGGCTAGGTTAAATATTACGTTTTCGCCTCAAGCCTTTGAAGATTATAAGTATTTTCAGCAGAACGATAAAAAAATGGT
GAAGAAGATTAATGAGTTACTTAAAAGTATTGACAGAAATGGTGCATTGGAAGGTATAGGTAAGCCTGAAAAGTTAAAAT
CGAATCTGACTGGGTATTATAGTAGACGTATCAATCACGAACATAGATTGGTTTATACAGTAGATGACAATCATATAAAA
ATAGCATCATGTAAATACCATTATTAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12201.39 Da        Isoelectric Point: 10.6058

>AT228361 WP_001172026.1 NZ_CP089533:22577-22912 [Pseudomonas veronii]
MQKRIIEGVEVQRSSGNVFADLGLPDAEKLKIKTGLVVEIRRAMRALGLTQQAAAKRMGIPQPKVSGMMRGDFTNLSERK
LMDCLNRLGYDIEIKVRPAAEPIGHLTLATA

Download         Length: 336 bp

>AT228361 NZ_CP123852:2362501-2362752 [Staphylococcus aureus]
ATGATTATTAAAAATTATTCATACGCTCGACAGAATTTAAAGGCACTTATGACAAAAGTAAATGATGATAGTGATATGGT
AACTGTAACATCTACTGATGATAAAAACGTAGTAATCATGTCAGAATCAGATTATAACTCCATGATGGAAACACTTTACC
TCCAACAGAACCCAAATAATGCTGAACACTTAGCTCAATCAATTGCAGATCTAGAACGTGGGAAAACTATAACGAAAGAT
ATAGATGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A024ELN5

Antitoxin

Source ID Structure
AlphaFold DB A0A024EKI7

References