Detailed information of TA system TA228356

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 208304..209343
• Replicon: chromosome
• Accession: NZ_CP089532
• Organism: Pseudomonas veronii strain G2

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: A0A2N7YN67
• Locus tag: LUW10_RS00935
• Protein ID: WP_046488818.1
• Coordinates: 208304..208879 (-)
• Length: 192 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: -
• Locus tag: LUW10_RS00940
• Protein ID: WP_126585954.1
• Coordinates: 208876..209343 (-)
• Length: 156 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LUW10_RS00915 (LUW10_00915)	203589..205211	+	1623	WP_232591094.1	VWA domain-containing protein	-
LUW10_RS00920 (LUW10_00920)	205250..205822	+	573	WP_017848620.1	RNA polymerase sigma factor	-
LUW10_RS00925 (LUW10_00925)	205819..206406	+	588	WP_198865665.1	hypothetical protein	-
LUW10_RS00930 (LUW10_00930)	206824..207888	+	1065	WP_226926362.1	2,3-butanediol dehydrogenase	-
LUW10_RS00935 (LUW10_00935)	208304..208879	-	576	WP_046488818.1	PIN domain-containing protein	Toxin
LUW10_RS00940 (LUW10_00940)	208876..209343	-	468	WP_126585954.1	helix-turn-helix domain-containing protein	Antitoxin
LUW10_RS00950 (LUW10_00950)	210025..211467	+	1443	WP_198717691.1	NADP-dependent succinate-semialdehyde dehydrogenase	-
LUW10_RS00955 (LUW10_00955)	211649..212926	+	1278	WP_017848615.1	4-aminobutyrate--2-oxoglutarate transaminase	-
LUW10_RS00960 (LUW10_00960)	213066..214274	+	1209	WP_046488795.1	HDOD domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (9-115)

Antitoxin

• SinI-like, DNA-binding domain (84-131)

Sequences

Toxin

Protein

• Length: 192 a.a.
• Molecular weight: 21807.98 Da
• Isoelectric Point: 5.0798

>T228356 WP_046488818.1 NZ_CP089532:c208879-208304 [Pseudomonas veronii]
MRHSPFTAFYDANVLYPAPLRDYLMHLALTGVYRARWSALIHEEWKRNLLINRPELTREQLDRTSSLMDKAVPDGIVTEY
EPLIEGLTLPDADDRHVLAAAIKCNASVIVTFNLKDFPKSALDAFDIEPLHPDDFIADLWDLDQAAVLEAAQRQRASLKN
PPHSAREYLDKLLQQKLPETVKLLSGFEFLI

Nucleotide

• Length: 576 bp

>T228356 NZ_CP123852:123739-123891 [Staphylococcus aureus]
ATGTCTAAAGACAAAGATCCAAAATTAAATTATCATGAAGAAGAAAACAGTATGGTAACTGATTTTGAAGATTTAAAAGA
ATTAGGTAAAGAAATGGAACAAATTTCTGATCAAAATGATCAAGAAAAGAATTCTGAAGAAGACAGTCAGTAA

Antitoxin

Protein

• Length: 156 a.a.
• Molecular weight: 17216.75 Da
• Isoelectric Point: 6.0048

>AT228356 WP_126585954.1 NZ_CP089532:c209343-208876 [Pseudomonas veronii]
MTTANLARTILPDEKEIAAAVASSRQLAAFLTTKFETQRIELVDETQHREVVELPTFALRLLGEILSELALGNAVKVVPI
HAELTTQEGADMLNVSRPHLVKLLDNGALPHTKTGRHRRIKFADLMTYKEQRDLASRNAMDELAAQAQELGMGYE

Nucleotide

• Length: 468 bp

>AT228356 NZ_CP123852:123115-123714 [Staphylococcus aureus]
ATGGCAATGAACTTTAAAGTCTTTGACAATAGTCAACTTGTAGCAGAATATGCTGCTGATATTATTAGAAAGCAATTTAA
CAATAATCCTACTACAATTGCAGGTTTTCATTTAGACACAGATCAAGCGCCAGTTCTAGATGAATTAAAGAAAAATGTTG
AAAAACATGCTGTTGATTTTAGCCAAATAAATATTTTAGATTATGACGATAAAAAATCATATTTCGAAGCGTTAGGTGTA
CCAGCAGGTCAAGTTTATCCAATTGCTTATGAAAAAGATGCAATCGAATTAATCGCTGATAAGATTAAAACTAAAGAAAA
TAAAGGGAAATTAACATTACAAGTTGTTTCTATCGATGAGCAAGGTAAGTTAAATGTTAGTATTCGTCAAGGACTAATGG
AAGCAAGAGAAATTTTCTTAGTAGTGACAGGTGCTAATAAACGAGATGTAGTTGAAAAATTATATCAAGAAAATGGTAAA
ACAAGCTTCGAACCAGCCGATTTAAAAGCACATAGAATGGTAAATGTTATTCTTGATAAAGAAGCGGCTGCAGGTTTACC
TGAAGATGTTAAAGCTTACTTTACGTCACGCTTTGCTTAA

Structures

Toxin

• AlphaFold DB: A0A2N7YN67