Detailed information of TA system TA228355

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 1297085..1298002
• Replicon: chromosome
• Accession: NZ_CP089530
• Organism: Bacillus amyloliquefaciens strain LS1-002-014s

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: -
• Locus tag: LUA14_RS06920
• Protein ID: WP_032866111.1
• Coordinates: 1297256..1298002 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: I2HQ14
• Locus tag: LUA14_RS06915
• Protein ID: WP_003154807.1
• Coordinates: 1297085..1297255 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LUA14_RS06875 (1292297)	1292297..1293886	+	1590	WP_205164551.1	hypothetical protein	-
LUA14_RS06880 (1293899)	1293899..1294324	+	426	WP_050498185.1	hypothetical protein	-
LUA14_RS06885 (1294329)	1294329..1294526	+	198	WP_007610833.1	XkdX family protein	-
LUA14_RS06890 (1294583)	1294583..1295344	+	762	WP_020955694.1	hypothetical protein	-
LUA14_RS06895 (1295396)	1295396..1295659	+	264	WP_003154815.1	hemolysin XhlA family protein	-
LUA14_RS06900 (1295673)	1295673..1295936	+	264	WP_003154813.1	phage holin	-
LUA14_RS06905 (1295950)	1295950..1296828	+	879	WP_020955695.1	N-acetylmuramoyl-L-alanine amidase	-
LUA14_RS06915 (1297085)	1297085..1297255	-	171	WP_003154807.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
LUA14_RS06920 (1297256)	1297256..1298002	-	747	WP_032866111.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
LUA14_RS06925 (1298106)	1298106..1299104	-	999	WP_020955696.1	inorganic phosphate transporter	-
LUA14_RS06930 (1299117)	1299117..1299734	-	618	WP_003154804.1	DUF47 domain-containing protein	-
LUA14_RS06935 (1300020)	1300020..1301336	-	1317	WP_007407254.1	amino acid permease	-
LUA14_RS06940 (1301659)	1301659..1302609	+	951	WP_218791607.1	ring-cleaving dioxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1262081..1306259	44178

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-239)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29095.60 Da
• Isoelectric Point: 4.7755

>T228355 WP_032866111.1 NZ_CP089530:c1298002-1297256 [Bacillus amyloliquefaciens]
MLMFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVLGSMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYAEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQNRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Nucleotide

• Length: 747 bp

>T228355 NZ_CP123852:19379-19555 [Staphylococcus aureus]
TTGGATAGATGGTGGCTATCTGAGTATAAGGAGGTGGTGCCTATGTTGGCATTACTGAAATCTTTAGAAAGGAGATGCCT
AATGATTACAATTAGTACCATGTTGCAGTTTGGTTTATTCCTTATTGCATTGATAGGTCTAGTAATCAAGCTTATTGAAT
TAAGCAATAAAAAATAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6676.74 Da
• Isoelectric Point: 10.9273

>AT228355 WP_003154807.1 NZ_CP089530:c1297255-1297085 [Bacillus amyloliquefaciens]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Nucleotide

• Length: 171 bp

>AT228355 NZ_CP123852:c19717-19543 [Staphylococcus aureus]
ATTTGATATTTATATTATGGTGTGTTAATTTATATATAGAAAAAGGGCAACATGCGCAAACATGTTACCCTAATGAGCCC
GTTAAAAAGACGGTGGCTATTTTAGATTAAAGATTAAATTAATAACCATTTAACCATCGAAACCAGCCAAAGTTAGCGAT
GGTTATTTTTTATTG

Structures

Antitoxin

• AlphaFold DB: I2HQ14