Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 485007..485644 Replicon chromosome
Accession NZ_CP089530
Organism Bacillus amyloliquefaciens strain LS1-002-014s

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag LUA14_RS02490 Protein ID WP_003156187.1
Coordinates 485294..485644 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag LUA14_RS02485 Protein ID WP_003156188.1
Coordinates 485007..485288 (+) Length 94 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LUA14_RS02465 (481372) 481372..481971 - 600 WP_003156193.1 rhomboid family intramembrane serine protease -
LUA14_RS02470 (482064) 482064..482429 + 366 WP_007410229.1 holo-ACP synthase -
LUA14_RS02475 (482594) 482594..483601 + 1008 WP_007410230.1 outer membrane lipoprotein carrier protein LolA -
LUA14_RS02480 (483718) 483718..484887 + 1170 WP_025284272.1 alanine racemase -
LUA14_RS02485 (485007) 485007..485288 + 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
LUA14_RS02490 (485294) 485294..485644 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
LUA14_RS02495 (485762) 485762..486583 + 822 WP_003156182.1 STAS domain-containing protein -
LUA14_RS02500 (486588) 486588..486953 + 366 WP_003156180.1 RsbT antagonist protein RsbS -
LUA14_RS02505 (486956) 486956..487357 + 402 WP_003156178.1 anti-sigma regulatory factor -
LUA14_RS02510 (487369) 487369..488376 + 1008 WP_052827076.1 PP2C family protein-serine/threonine phosphatase -
LUA14_RS02515 (488440) 488440..488769 + 330 WP_003156176.1 anti-sigma factor antagonist RsbV -
LUA14_RS02520 (488766) 488766..489248 + 483 WP_003156173.1 anti-sigma B factor RsbW -
LUA14_RS02525 (489214) 489214..490002 + 789 WP_003156171.1 RNA polymerase sigma factor SigB -
LUA14_RS02530 (490002) 490002..490604 + 603 WP_007410234.1 PP2C family serine/threonine-protein phosphatase -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(4-111)

Antitoxin

(12-45)


Sequences


Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T228354 WP_003156187.1 NZ_CP089530:485294-485644 [Bacillus amyloliquefaciens]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T228354 NZ_CP123849:361622-362119 [Agrobacterium fabacearum]
GTGACTCTCAGCGCACCAGTCCCCCTGGCCGATCATCACGAACTGGCCGAATTCAATTCTGGAGTACCTGAACTGGACGA
CTGGCTTCGGCGCCGTGCCCGGGCCAATCAAGCAGGCGGCGCGTCGCGGACCTTTGTCGTTTGCGACGAGAGCCGCGTCA
TCGCGTACTACGCCCTGGCCTCTGGCTCCGTGAAACCGCCGGAAGTGCCTGGTCGCTTTCGGCGCAATATGCCTGATCCA
ATCCCAGTTGCAGTGCTTGGCCGCCTCGCCATTGATCAATCCTGTCAAGGGCGTGGTATCGGCAGGGCATTGGTGCGCGA
CGCGGGTTTGCGCCTGCTGAATGCCGCTGAAGTGCTCGGGATCCGCGGCGTGCTGGTCCATGCGATTTCTGATGACGCAC
GAGCCTTCTACGAAGCCGTCGGCTTCCTGTCTTCTCCGTCCGATCCCATGATGCTGATGGTCGGTTTGCACGACCTCAAT
AACGCGCTGAACACCTAA

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT228354 WP_003156188.1 NZ_CP089530:485007-485288 [Bacillus amyloliquefaciens]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT228354 NZ_CP123849:361329-361625 [Agrobacterium fabacearum]
ATGCCGACCTCACACACGGGTAAGAGCCCGGACGCAAGTGTGCCGCTGAATATGCGCATCAAACCTGCTACTCGCAACTT
GATCGATCGCGCCGCGGAGTTGCTCGGCAAAACGCGCACAGATTTCATGTTGGAAGCTTCCGAGCGCCGTGCGGAGGAGG
TGCTGCTCGACCGTACAATCGTCACGGTAAGCCCCGAAATTTATGCCGAATATCTTGCCCGTCTCGATGCGCCTGCACAG
CCCAATGAGCGTCTAAAGCGCACGATGTCGACCAAAGCGCCCTGGGATGAGGCGTGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8


Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References