Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 245768..246410 Replicon chromosome
Accession NZ_CP089518
Organism Bacillus cereus strain PT1

Toxin (Protein)


Gene name pemK Uniprot ID R8I8B8
Locus tag LUS72_RS01380 Protein ID WP_000635965.1
Coordinates 246060..246410 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag LUS72_RS01375 Protein ID WP_000004570.1
Coordinates 245768..246055 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LUS72_RS01350 241080..242042 + 963 WP_097831059.1 UV DNA damage repair endonuclease UvsE -
LUS72_RS01355 242035..242607 - 573 WP_097831058.1 rhomboid family intramembrane serine protease -
LUS72_RS01360 242700..243059 + 360 WP_097831057.1 holo-ACP synthase -
LUS72_RS01365 243216..244166 + 951 WP_141533555.1 outer membrane lipoprotein carrier protein LolA -
LUS72_RS01370 244285..245454 + 1170 WP_097831055.1 alanine racemase -
LUS72_RS01375 245768..246055 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
LUS72_RS01380 246060..246410 + 351 WP_000635965.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
LUS72_RS01385 246479..248647 + 2169 WP_141533554.1 Tex family protein -
LUS72_RS01390 248705..248821 - 117 WP_001143642.1 cortex morphogenetic protein CmpA -
LUS72_RS01395 249030..249491 + 462 WP_097831053.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12948.04 Da        Isoelectric Point: 5.7168

>T228349 WP_000635965.1 NZ_CP089518:246060-246410 [Bacillus cereus]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMSRVDEALQISLGLIDF

Download         Length: 351 bp

>T228349 NZ_CP123848:c7938-6697 [Agrobacterium fabacearum]
ATGACAACCATCTACTACGAGACCTTGCCTGTCGCCCATCTGACTTTTGAAGGCGAATGGAGGTTGGACTATGATCCGGG
CTGGGAAGCACGCCGCCCGGCCTTTCCGGTGTCGCTTACCATGCCGCTGCGTTCCGGATCCGTAGGTGCAGCGAAGCTGC
TTCCCTGGCTTGCCAATCTGCTTCCGGAAACCCATCTTGCCGAGATCGGTCAGCGCTTCAAGGTTTCCCCGCAAGATATT
GTCGGTCTACTTGCTCATATCGGACGTGACACGGCGGGGGCACTGTCGATCGGGGAGCCGCGGAAAGCTGGCGTCAACCT
CCGGCCGGTGCCGGACGAGCAGGCGCTGGAGCGTATTCTCAATGAACTTCCTGCCAAACCCTTTCTGGTCGGAGAGCGCG
GTGTTTCCATGTCGCTTGCTGGCGTCCAGGAGAAACTGCCGGTGTTTGTTGACGGGAACGGCCGTATTTCAGTTCCGGTT
GATGGGACGCCATCCACGCATATTCTCAAGCCGGATACGAAACGCCTCGCGGGGAGCGTGGAGAACGAAGCCTTTTGCCT
GTCGCTTGCACGTGCCTGCGGTCTTGAGGCTGCCGAGGTGACGATCGGAGTAGCTGGCAAGCGACGTTACCTTCTCGTGA
AACGCTGTGATCGCTTTACCGACGCTCAAGGCGAGATCCGCAGGCTGCATCAGGAGGATCTTTGCCAGTTAACGGGTTAC
TTCCCCTCGCAGAAATACGAGCGGTCCTCGACCGGCGGCGGTGTGACCATGAAGATGATGTTTGATGCCGTCAGCGATCT
TGTTTCGCCTGGCGAGCGTTTGAAACTTCTCGATGCGGTGATCTTCAATGTCCTGATCTGCAATTCGGATTCGCATGCCA
AAAATTACTCTATCCTGATTGGTGCCGGTGGGTCCGCGAAGATGGCTCCACTCTATGACCTGATGTGCGCTGCAGTTTAT
CGCCAGGTCGACCAGAGCCTGCCGCAGGGGATTTCCGGTCGGTTCCATGCGCCTGACCTGCGGCGTGCCGACTGGCAGGC
ACTGGCTGATGACATTGGATTGAGCGGCGCATCGACACTGAAACGGGTGGAGGAGCTTGCAAAACTGGTCAGCGTCGCAT
GCGAAGAGGTGGCACCACATATCGTCGCCCTCTCCCGCCATCCGACGAGGATTCTTGAGCGAATTACTCACAGCGTTCAA
AAGCGGTGCCGGCGAATTCAGGAGCAGATGCATGATCGATGA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT228349 WP_000004570.1 NZ_CP089518:245768-246055 [Bacillus cereus]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT228349 NZ_CP123848:c8207-7935 [Agrobacterium fabacearum]
ATGTTTAGGTCCGCGCGTGATTTCGGCGCTGCTATCAAGGAGAGACGCAAAGCACTTGGCTGGACGCAAACCGGTCTTGC
TGCCCGTTCCGGAACGGGGGAACGCTTCATCGTCGAGCTTGAATCGGGCAAGCCGAGTTGCCAGCTCGAAAAAGCCCTGA
TCGTCGCACGCGCAGTTGGAATCGATCTTGGCGATCTCAAGACGGTTCAGGCTGTGCAAGTCGCGTCGGACGATGACCTC
GGCTTTTTGCCGATGTTTGGTGCTGATCAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A366G118

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References