Detailed information of TA system TA228348

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2305546..2306182
• Replicon: chromosome
• Accession: NZ_CP089514
• Organism: Bacillus subtilis strain SR-7

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: LUM37_RS12100
• Protein ID: WP_003156187.1
• Coordinates: 2305832..2306182 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G4NU32
• Locus tag: LUM37_RS12095
• Protein ID: WP_003225183.1
• Coordinates: 2305546..2305827 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LUM37_RS12065 (LUM37_12065)	2301271..2302443	+	1173	WP_014479474.1	cystathionine beta-lyase	-
LUM37_RS12070 (LUM37_12070)	2302476..2303021	-	546	WP_161985924.1	GNAT family protein	-
LUM37_RS12075 (LUM37_12075)	2303091..2304282	-	1192	Protein_2387	DUF819 family protein	-
LUM37_RS12085 (LUM37_12085)	2304735..2304965	-	231	WP_232566814.1	hypothetical protein	-
LUM37_RS12090 (LUM37_12090)	2305368..2305484	-	117	Protein_2389	hypothetical protein	-
LUM37_RS12095 (LUM37_12095)	2305546..2305827	+	282	WP_003225183.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
LUM37_RS12100 (LUM37_12100)	2305832..2306182	+	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
LUM37_RS12105 (LUM37_12105)	2306298..2307122	+	825	WP_009966610.1	RsbT co-antagonist protein RsbRA	-
LUM37_RS12110 (LUM37_12110)	2307127..2307492	+	366	WP_003225190.1	RsbT antagonist protein RsbS	-
LUM37_RS12115 (LUM37_12115)	2307496..2307897	+	402	WP_003225192.1	serine/threonine-protein kinase RsbT	-
LUM37_RS12120 (LUM37_12120)	2307909..2308916	+	1008	WP_134991884.1	phosphoserine phosphatase RsbU	-
LUM37_RS12125 (LUM37_12125)	2308985..2309314	+	330	WP_014475877.1	anti-sigma factor antagonist RsbV	-
LUM37_RS12130 (LUM37_12130)	2309311..2309793	+	483	WP_077671275.1	anti-sigma B factor RsbW	-
LUM37_RS12135 (LUM37_12135)	2309759..2310547	+	789	WP_003246715.1	RNA polymerase sigma factor SigB	-
LUM37_RS12140 (LUM37_12140)	2310547..2311146	+	600	WP_095252677.1	phosphoserine phosphatase RsbX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T228348 WP_003156187.1 NZ_CP089514:2305832-2306182 [Bacillus subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T228348 NZ_CP123847:c344503-344252 [Agrobacterium fabacearum]
GTGATCTGGACAATTGAATACGATGTTCTTGTCCAGAAAGAGATGCGGAAAATCGATCCGCAGACGCGTCAGCGTATCCG
AGTAGCGGCGCTCGACAATCCCCGCCAGACAGGTGCCGCCCTGCAAGGCTCAGAACTCGGCAGCTTCTGGCGTTACCGGG
TAGGCGATTACCGTATCATCTGCGATATACAGGACCACAAGCTTGTCGTGCTGGTGGTGGAAATTGGCCATCGCCGCGAA
ATTTACCGTTAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10546.92 Da
• Isoelectric Point: 6.4708

>AT228348 WP_003225183.1 NZ_CP089514:2305546-2305827 [Bacillus subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT228348 NZ_CP123847:c344714-344487 [Agrobacterium fabacearum]
ATGAGCAAACAGACTGCCATCCGCCTGCCGGACGAGACCTATGAACGGCTGAAAGCGCTTTCCGAGCGCACGGGCCGTAC
GTCCGCATACTATATCCGCGAGGCGATTGAGAAACATATTGAGGATATGGAAGATCTCTATCTAGCCGAAGAGGCGACGC
GGCGCATCCAGCGCGGTGAATCAAAGGTGGTAAGCGCCGAGGAGTTCTGGCGTGATCTGGACAATTGA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• PDB: 4ME7
• AlphaFold DB: G4NU32