Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | spoIISABC/SpoIISA-SpoIISB |
| Location | 1721229..1722145 | Replicon | chromosome |
| Accession | NZ_CP089514 | ||
| Organism | Bacillus subtilis strain SR-7 | ||
Toxin (Protein)
| Gene name | spoIISA | Uniprot ID | O34853 |
| Locus tag | LUM37_RS08945 | Protein ID | WP_003244695.1 |
| Coordinates | 1721399..1722145 (-) | Length | 249 a.a. |
Antitoxin (Protein)
| Gene name | spoIISB | Uniprot ID | G4EXD8 |
| Locus tag | LUM37_RS08940 | Protein ID | WP_003232646.1 |
| Coordinates | 1721229..1721399 (-) | Length | 57 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| LUM37_RS08895 (LUM37_08895) | 1716881..1717024 | - | 144 | Protein_1757 | DNA cytosine methyltransferase | - |
| LUM37_RS08900 (LUM37_08900) | 1717264..1718073 | + | 810 | Protein_1758 | hypothetical protein | - |
| LUM37_RS08905 (LUM37_08905) | 1718091..1718417 | + | 327 | WP_172861714.1 | XkdW family protein | - |
| LUM37_RS08910 (LUM37_08910) | 1718414..1718578 | + | 165 | WP_014479563.1 | XkdX family protein | - |
| LUM37_RS08915 (LUM37_08915) | 1718625..1719464 | + | 840 | WP_172861716.1 | phage-like element PBSX protein XepA | - |
| LUM37_RS08920 (LUM37_08920) | 1719517..1719786 | + | 270 | WP_014479565.1 | hemolysin XhlA family protein | - |
| LUM37_RS08925 (LUM37_08925) | 1719799..1720062 | + | 264 | WP_014479566.1 | phage holin | - |
| LUM37_RS08930 (LUM37_08930) | 1720075..1720968 | + | 894 | WP_015715742.1 | N-acetylmuramoyl-L-alanine amidase | - |
| LUM37_RS08935 (LUM37_08935) | 1721006..1721143 | - | 138 | WP_003232648.1 | three component toxin-antitoxin-antitoxin system antitoxin SpoIISC | - |
| LUM37_RS08940 (LUM37_08940) | 1721229..1721399 | - | 171 | WP_003232646.1 | three component toxin-antitoxin-antitoxin system antitoxin SpoIISB | Antitoxin |
| LUM37_RS08945 (LUM37_08945) | 1721399..1722145 | - | 747 | WP_003244695.1 | toxin-antitoxin-antitoxin system toxin SpoIISA | Toxin |
| LUM37_RS08950 (LUM37_08950) | 1722255..1723256 | - | 1002 | WP_070548855.1 | inorganic phosphate transporter | - |
| LUM37_RS08955 (LUM37_08955) | 1723269..1723886 | - | 618 | WP_003218470.1 | DUF47 domain-containing protein | - |
| LUM37_RS08960 (LUM37_08960) | 1724162..1725478 | - | 1317 | WP_015252262.1 | serine/threonine exchanger | - |
| LUM37_RS08965 (LUM37_08965) | 1725867..1726817 | + | 951 | WP_015252261.1 | ring-cleaving dioxygenase | - |
| LUM37_RS08970 (LUM37_08970) | 1726926..1727027 | + | 102 | Protein_1772 | hypothetical protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Prophage | - | - | 1707531..1730545 | 23014 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 249 a.a. Molecular weight: 29061.50 Da Isoelectric Point: 4.6191
>T228347 WP_003244695.1 NZ_CP089514:c1722145-1721399 [Bacillus subtilis]
MVLFFQIMVWCIVAGLGLYVYATWRFEAKVKEKMSAIRKTWYLLFVLGAMVYWTYEPTSLFTHWERYLIVAVSFALIDAF
IFLSAYVKKLAGSELETDTREILEENNEMLHMYLNRLKTYQYLLKNEPIHVYYGSIDAYAEGIDKLLKTYADKMNLTASL
CHYSTQADKDRLTEHMDDPADVQTRLDRKDVYYDQYGKVVLIPFTIETQNYVIKLTSDSIVTEFDYLLFTSLTSIYDLVL
PIEEEGEG
MVLFFQIMVWCIVAGLGLYVYATWRFEAKVKEKMSAIRKTWYLLFVLGAMVYWTYEPTSLFTHWERYLIVAVSFALIDAF
IFLSAYVKKLAGSELETDTREILEENNEMLHMYLNRLKTYQYLLKNEPIHVYYGSIDAYAEGIDKLLKTYADKMNLTASL
CHYSTQADKDRLTEHMDDPADVQTRLDRKDVYYDQYGKVVLIPFTIETQNYVIKLTSDSIVTEFDYLLFTSLTSIYDLVL
PIEEEGEG
Download Length: 747 bp
>T228347 NZ_CP123843:c2025574-2025290 [Agrobacterium fabrum]
ATGCAGCAAAAGGGTACGATTGCTCTCGGCTGGAGCCACGAAGGTTGGGAAGATTATCTTCACTGGCAGAAATATGATCG
CAAGATGCTTGAACGCATCAACGAACTCATAAAGGACTGTCGGCGACATCCCTTCGAGGGCATCGGGAAACCAGAGCCTC
TGCGTTTCCAGTTGAAGGGCCTGTGGTCCCGCCGGATTTCGCAGGAGCATCGGCTTGTCTATGAGCTTCGCGGCGACGGT
GAAAAGGCGGTGCTGATTATCCACCAATGCCGCTTCCACTACTGA
ATGCAGCAAAAGGGTACGATTGCTCTCGGCTGGAGCCACGAAGGTTGGGAAGATTATCTTCACTGGCAGAAATATGATCG
CAAGATGCTTGAACGCATCAACGAACTCATAAAGGACTGTCGGCGACATCCCTTCGAGGGCATCGGGAAACCAGAGCCTC
TGCGTTTCCAGTTGAAGGGCCTGTGGTCCCGCCGGATTTCGCAGGAGCATCGGCTTGTCTATGAGCTTCGCGGCGACGGT
GAAAAGGCGGTGCTGATTATCCACCAATGCCGCTTCCACTACTGA
Antitoxin
Download Length: 57 a.a. Molecular weight: 6654.72 Da Isoelectric Point: 11.0203
>AT228347 WP_003232646.1 NZ_CP089514:c1721399-1721229 [Bacillus subtilis]
MERAFQNRCEPRAAKPFKILKKRSTTSVASYQVSPHTARIFKENERLIDEYKRKKA
MERAFQNRCEPRAAKPFKILKKRSTTSVASYQVSPHTARIFKENERLIDEYKRKKA
Download Length: 171 bp
>AT228347 NZ_CP123843:c2025877-2025626 [Agrobacterium fabrum]
ATGCGTACTGTTATTTTCTCGAAAGCACGCGAATCCCTTGCATCGCTTCTGGATGAAGTGTCGCAGGATCGGGTGGCTGT
GGAAATTGTACGGCGTGACAAGCCGTCCGCCGTTCTTATCGATAAGGACGAATACGAAGGCTTGCTGGAAACCTTGCATC
TATGCTCGTCACCAGAGAATTTTGCCAGACTTAGAAAGTCGATGTCTGACGTGGATACTGGTGTTTTCGAAAGCCATGAT
CTCATCGACTGA
ATGCGTACTGTTATTTTCTCGAAAGCACGCGAATCCCTTGCATCGCTTCTGGATGAAGTGTCGCAGGATCGGGTGGCTGT
GGAAATTGTACGGCGTGACAAGCCGTCCGCCGTTCTTATCGATAAGGACGAATACGAAGGCTTGCTGGAAACCTTGCATC
TATGCTCGTCACCAGAGAATTTTGCCAGACTTAGAAAGTCGATGTCTGACGTGGATACTGGTGTTTTCGAAAGCCATGAT
CTCATCGACTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|