Detailed information of TA system TA228346

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 99715..100451
• Replicon: plasmid pHNGS471-1
• Accession: NZ_CP089510
• Organism: Klebsiella pneumoniae strain GD21SC417

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: A0A8T5ZF70
• Locus tag: LT986_RS26225
• Protein ID: WP_004187044.1
• Coordinates: 99969..100451 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: LT986_RS26220
• Protein ID: WP_003026799.1
• Coordinates: 99715..99981 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LT986_RS26205 (LT986_26195)	95187..96581	+	1395	WP_032439652.1	cytosine permease	-
LT986_RS26210 (LT986_26200)	96593..97801	+	1209	WP_032448288.1	imidazolonepropionase	-
LT986_RS26215 (LT986_26205)	97794..98603	+	810	WP_023329017.1	N-formylglutamate deformylase	-
LT986_RS26220 (LT986_26210)	99715..99981	+	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
LT986_RS26225 (LT986_26215)	99969..100451	+	483	WP_004187044.1	GNAT family N-acetyltransferase	Toxin
LT986_RS26230 (LT986_26220)	100662..102008	+	1347	WP_077251107.1	ISNCY family transposase	-
LT986_RS26235 (LT986_26225)	102168..102872	+	705	WP_040167821.1	toll/interleukin-1 receptor domain-containing protein	-
LT986_RS26240 (LT986_26230)	103149..104495	+	1347	WP_077254728.1	ISNCY family transposase	-
LT986_RS26245 (LT986_26235)	104544..104942	+	399	WP_032422684.1	helix-turn-helix domain-containing protein	-
LT986_RS26250 (LT986_26240)	105090..105200	-	111	Protein_104	IS3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(A) / aph(3')-Ia / dfrA12 / aadA2 / cmlA1 / ant(3'')-Ia / sul3 / mef(B)	iutA / iucD / iucC / iucB / iucA	1..239834	239834

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (46-148)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17280.93 Da
• Isoelectric Point: 8.7197

>T228346 WP_004187044.1 NZ_CP089510:99969-100451 [Klebsiella pneumoniae]
VGCITAPEPLSSAHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTYERTLFLKLP

Nucleotide

• Length: 483 bp

>T228346 NZ_CP123843:c1964418-1964149 [Agrobacterium fabrum]
ATGGATTTCGAATTTGATCCTGCCAAAAGTGAAAGTAACAAAGACAAGCACGGAATAGATTTTGTCGAGGCAAGAGGGCT
TTGGCTGGACGAGAAAAGACTTGTCGTCCCTCTCGAAACCACGCTGGAAGAGCGGTATATTATGATTGCTCAGTTGCGTG
GGAAGTGCTGGAGCGCCGTTTACACCTATAGACATGACCGCGTGCGGATCATCTCTGTCAGGCGTTCCAGAGACAAGGAG
AAGCAGCGTTATGAAGACGATCAGCGCTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT228346 WP_003026799.1 NZ_CP089510:99715-99981 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT228346 NZ_CP123843:c1964168-1963926 [Agrobacterium fabrum]
ATGAAGACGATCAGCGCTGAAGAGTTCGACAGGAAATTCGACGAAGGCGAAGACATCGACGATTATCTCGATTGGTCGAA
GGCTAGCCGTCCCGGGCTGGAAATGGTGCATGTCGATGTCGATCTTCCTGAAAAGGTTTTGCGCAGAGTGGATGCGGAGG
CAGCACGCCTGGGCATGACGCGGCAGTCCCTTATGGCTAAATGGATTGCCGAAAGCCTTGAGGCTGGCAGGGTCGGAAAA
TAA

Structures

Antitoxin

• AlphaFold DB: A0A1Q8YL66