Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4581795..4582605 Replicon chromosome
Accession NZ_CP089509
Organism Klebsiella pneumoniae strain GD21SC417

Toxin (Protein)


Gene name KacT Uniprot ID A0A483G813
Locus tag LT986_RS22525 Protein ID WP_023279404.1
Coordinates 4581795..4582328 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag LT986_RS22530 Protein ID WP_002887278.1
Coordinates 4582339..4582605 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LT986_RS22520 (4580626) 4580626..4581747 + 1122 WP_002887282.1 cupin domain-containing protein -
LT986_RS22525 (4581795) 4581795..4582328 - 534 WP_023279404.1 type II toxin-antitoxin system toxin KacT Toxin
LT986_RS22530 (4582339) 4582339..4582605 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
LT986_RS22535 (4582708) 4582708..4584141 - 1434 WP_004192214.1 Cu(+)/Ag(+) sensor histidine kinase -
LT986_RS22540 (4584131) 4584131..4584814 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
LT986_RS22545 (4584986) 4584986..4586371 + 1386 WP_004192218.1 efflux transporter outer membrane subunit -
LT986_RS22550 (4586389) 4586389..4586733 + 345 WP_004192220.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19809.71 Da        Isoelectric Point: 6.2369

>T228341 WP_023279404.1 NZ_CP089509:c4582328-4581795 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDKNSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T228341 NZ_CP123840:166652-166945 [Agrobacterium fabacearum]
ATGCCATTTAGCCTATCTGTCCAAGCGGAAGAGGATATTATTTCCATTGCAGAAGAATGCATCCGCATCTTCGGAGCACT
AGTCGCCAGACAATATCACGGTGAGCTTTTTGCCTTGCTCGAACTTATCGCCACCAACCCAAGAATGGCACGCGAACGCC
ATGAGATTTCTCCTCCTGTCAGGATCCATCCTTTCAAGGCTCACCTTGTCGTGTACCGCATCATTGAAGACGGGAGCGTA
TTCGTCATCCGCATACGGCACGGCCATGAACACTGGGCGGGAGATTCTTTCTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT228341 WP_002887278.1 NZ_CP089509:c4582605-4582339 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT228341 NZ_CP123840:166398-166652 [Agrobacterium fabacearum]
ATGGCGACGATGAATGTATCCCTGCCAGACCCCATGAAAGATTGGGTTGAAACACAGGCCAAGACTGGTCGATACTCGAA
TGCGAGCGATTATGTCCGGGACCTGATCCGCAGAGACCAAGCGCGTACGGACAAGATTGCCGAGATGCAGCGATTTGTCG
ACGACGGGATCAGAAGCGGAGTTGGGAGCCGTTCAACGGAAGAGTTGTTTGCTTTAGCCACTTCTCGAGTTGACATCAGC
CGCGGACGCGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A483G813

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References