Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3982813..3983432 Replicon chromosome
Accession NZ_CP089509
Organism Klebsiella pneumoniae strain GD21SC417

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag LT986_RS19655 Protein ID WP_002892050.1
Coordinates 3983214..3983432 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag LT986_RS19650 Protein ID WP_002892066.1
Coordinates 3982813..3983187 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LT986_RS19640 (3977965) 3977965..3979158 + 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
LT986_RS19645 (3979181) 3979181..3982327 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
LT986_RS19650 (3982813) 3982813..3983187 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
LT986_RS19655 (3983214) 3983214..3983432 + 219 WP_002892050.1 HHA domain-containing protein Toxin
LT986_RS19660 (3983591) 3983591..3984157 + 567 WP_002892030.1 maltose O-acetyltransferase -
LT986_RS19665 (3984129) 3984129..3984269 - 141 WP_004147370.1 hypothetical protein -
LT986_RS19670 (3984290) 3984290..3984760 + 471 WP_002892026.1 YlaC family protein -
LT986_RS19675 (3984735) 3984735..3986186 - 1452 WP_021313732.1 PLP-dependent aminotransferase family protein -
LT986_RS19680 (3986287) 3986287..3986985 + 699 WP_002892021.1 GNAT family protein -
LT986_RS19685 (3986982) 3986982..3987122 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
LT986_RS19690 (3987122) 3987122..3987385 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T228340 WP_002892050.1 NZ_CP089509:3983214-3983432 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T228340 NZ_CP123784:c1343349-1343050 [Minwuia sp. IMCC4030]
ATGCCGGATAGCGATCCGGGCTACCGGCTTTCTCCGCTCGCGAGAACCGATCTGGAAGACATCTGGCGCTACACCTTCGA
GAACTGGTCGCTTGAACAGGCGGATCGCTACCATGGCACGATCATGACCGCGATCCTGGCGTTGGCTGATGGCTCAGTAT
CGGGTAGCCGCGCCGACGTCCGTGAGGGCTATTTCAAGCTAAGAGCCGGTTCCCATGTCCTGTTCTACCGAAAGGCCGAC
ACCACTCTGGAGATCATGCGCATCCTGCATCAGCGCATGGACGTGGATCGACACCTCTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT228340 WP_002892066.1 NZ_CP089509:3982813-3983187 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT228340 NZ_CP123784:c1343584-1343339 [Minwuia sp. IMCC4030]
ATGGCACGCAATACCTCGGTCTCGCTTGGTGATCATTTCACCGGCTTCATTGACAGTCAGGTTCGGGACGGTCGCTACGG
CTCCGCCAGCGATGTGGTCAGGGCTGGTCTGCGGCTGCTGGAGGAACATGAAACCAGGGTCCGAGCCTTGCAGAACGCCC
TGATCGCCGCAGAGGAATCCGGTCCCCCAGAAGCTTTCGACAGCGATGCCTTCCTGGAGGAAATGCGGAAGAAGCATGCC
GGATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References