Detailed information of TA system TA228325

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 5211094..5211670
• Replicon: chromosome
• Accession: NZ_CP089448
• Organism: Klebsiella michiganensis strain 8-1

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: -
• Locus tag: LT930_RS24490
• Protein ID: WP_064375702.1
• Coordinates: 5211383..5211670 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: A0A0H3H591
• Locus tag: LT930_RS24485
• Protein ID: WP_014227757.1
• Coordinates: 5211094..5211396 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LT930_RS24470 (LT930_24470)	5207925..5208260	+	336	WP_064375703.1	endoribonuclease SymE	-
LT930_RS24475 (LT930_24475)	5208722..5209633	+	912	WP_064378388.1	acetamidase/formamidase family protein	-
LT930_RS24480 (LT930_24480)	5209630..5210973	+	1344	WP_049101852.1	APC family permease	-
LT930_RS24485 (LT930_24485)	5211094..5211396	-	303	WP_014227757.1	BrnA antitoxin family protein	Antitoxin
LT930_RS24490 (LT930_24490)	5211383..5211670	-	288	WP_064375702.1	BrnT family toxin	Toxin
LT930_RS24495 (LT930_24495)	5211918..5212361	-	444	WP_064375701.1	FosA family fosfomycin resistance glutathione transferase	-
LT930_RS24500 (LT930_24500)	5212355..5213263	-	909	WP_064375700.1	LysR family transcriptional regulator	-
LT930_RS24505 (LT930_24505)	5213351..5214133	+	783	WP_064375699.1	NAD(P)H-dependent oxidoreductase	-
LT930_RS24510 (LT930_24510)	5214281..5214865	+	585	WP_004098242.1	TetR/AcrR family transcriptional regulator	-
LT930_RS24515 (LT930_24515)	5215011..5215811	+	801	WP_064375698.1	helix-turn-helix domain-containing protein	-
LT930_RS24520 (LT930_24520)	5215808..5216326	+	519	WP_004098240.1	FidL-like protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (38-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11210.69 Da
• Isoelectric Point: 7.4687

>T228325 WP_064375702.1 NZ_CP089448:c5211670-5211383 [Klebsiella michiganensis]
MPMEFEWDANKAISNLRKHGIRFEEAVLVFDDPRHLSRQDRYENGEYRWQTLGLVHGIIVIMVAHSVRFESGTEVIRIIS
ARKADSKERNRYEHG

Nucleotide

• Length: 288 bp

>T228325 NZ_CP123767:40166-40453 [Acinetobacter pittii]
ATGGAACAGTATTTTGAATGGGATGAGGCTAAGAATCGAAAGAATCAGAAAAAGCATGATATCTCTTTCGAAACTGCAAG
CCTTGTTTTTGAAGATCCATTACGGATCTCAATCCAAGACAGACATACCGATGGTGAAGAACGTTGGCAAACCATTGGAC
GAGTTAAAGGCGTACTAATGTTATTGGTGGCTCACACCATTTTTGATGAAGATGACTGTGAAATCATACGAATCATTAGT
GCAAGGCAAGTGACTAAAGCGGAGCGAAACAAATATGAGCATGGTTAG

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11607.18 Da
• Isoelectric Point: 9.4429

>AT228325 WP_014227757.1 NZ_CP089448:c5211396-5211094 [Klebsiella michiganensis]
MNMVKHKRDNPDTLTAKRDDELKALANKTDDEIDYSDIPATDDAQWSDAVRGKFYRPLKTQASVRIDADIMEWLKRPGKG
YQTRLNAILREAMIRDLNKK

Nucleotide

• Length: 303 bp

>AT228325 NZ_CP123767:40440-40754 [Acinetobacter pittii]
ATGAGCATGGTTAGATACTCACGCAAAGAGCTGAATGAGAAATTTGGTGAGAAGCAAGATGCTGAAATTCAACGCTTGCT
TGCTAAAGGCACGGTTCCTGATGATCAGCTAGATCTATCAGATATTCCTGAAATTACCGATTGGAGCAGTGCTGTACGCC
AAAATCAATTTTACCGCCCAGTGAAGCAACAAACTTCAATTCGGTTAGATGCTGATGTACTTGCTTGGTTCAAAGCACAA
GGCAAAGGTTATCAAACACGAATGAATGAAATTTTACGGGATGCTATGCTGAAAGAATTAAAAAATCATCAATAA

Structures

Antitoxin

• AlphaFold DB: A0A0H3H591