Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4524108..4524727 Replicon chromosome
Accession NZ_CP089448
Organism Klebsiella michiganensis strain 8-1

Toxin (Protein)


Gene name Hha Uniprot ID H3N9D8
Locus tag LT930_RS21335 Protein ID WP_004099646.1
Coordinates 4524509..4524727 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID A0A0J2I7Z1
Locus tag LT930_RS21330 Protein ID WP_025107145.1
Coordinates 4524108..4524482 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LT930_RS21320 (LT930_21320) 4519264..4520457 + 1194 WP_004136054.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
LT930_RS21325 (LT930_21325) 4520480..4523626 + 3147 WP_014228207.1 multidrug efflux RND transporter permease subunit AcrB -
LT930_RS21330 (LT930_21330) 4524108..4524482 + 375 WP_025107145.1 Hha toxicity modulator TomB Antitoxin
LT930_RS21335 (LT930_21335) 4524509..4524727 + 219 WP_004099646.1 HHA domain-containing protein Toxin
LT930_RS21340 (LT930_21340) 4524890..4525456 + 567 WP_032748289.1 maltose O-acetyltransferase -
LT930_RS21345 (LT930_21345) 4525428..4525562 - 135 WP_223226764.1 hypothetical protein -
LT930_RS21350 (LT930_21350) 4525583..4526053 + 471 WP_064357998.1 YlaC family protein -
LT930_RS21355 (LT930_21355) 4526028..4527482 - 1455 WP_064358105.1 PLP-dependent aminotransferase family protein -
LT930_RS21360 (LT930_21360) 4527584..4528282 + 699 WP_064374550.1 GNAT family protein -
LT930_RS21365 (LT930_21365) 4528279..4528419 - 141 WP_003859006.1 type B 50S ribosomal protein L36 -
LT930_RS21370 (LT930_21370) 4528419..4528682 - 264 WP_004848323.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-115)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8640.05 Da        Isoelectric Point: 8.9008

>T228323 WP_004099646.1 NZ_CP089448:4524509-4524727 [Klebsiella michiganensis]
MSDKTLTKIDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPPSVWKFIR

Download         Length: 219 bp

>T228323 NZ_CP123767:19929-20285 [Acinetobacter pittii]
ATGTGGATAGTCATTACGACAGATCTATTTAATGAATGGCTTGAACAGCAAGACGAATCAACACAAGAGAAGGTTCTGGC
TGCCCTAGTTGTTCTACAGCAGCAAGGACCAAGTTTAGGCCGCCCTTTAGTAGATACTGTCTATGACTCTAAATTTACCA
ACATGAAAGAGCTTCGTGTTCAACATCGAGGTAAACCACTGAGAGCTTTCTTCGCATTTGATCCTTTACGACAGGCTATT
GTTCTATGTATTGCTGATAAAGGCAGTAAAAAGCGTTTTTATAAAGACATGCTGGATATTGCAGATGAACAATACCAACT
TCATCTCACCACCCTAGGAGATAAATCTAATGGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14366.15 Da        Isoelectric Point: 4.8989

>AT228323 WP_025107145.1 NZ_CP089448:4524108-4524482 [Klebsiella michiganensis]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIAAFALNYKIKYAEDNKLITQIDEYL
DDTFVLFSNYGINSADLQKWRKSGNRLFRCFVNACRENPASLSC

Download         Length: 375 bp

>AT228323 NZ_CP123767:20278-20580 [Acinetobacter pittii]
ATGGCTAAGACTCTGCAAGAATTGTTAGCTAGCCGCTCTCCAGAGAGCCAAGCCCGTATTCAAAAAATGGCAGATGAATT
ACTGCTAGAAAATCAGCTTCATCTTATTCGTGAAGAACTTGAAATCTCTCAAAAGGAACTTGCTGAAACTTTGGGAATAA
AACAGCCATCGCTTTCAGCAATAGAAAATCGTGGCAATGATCTTAAGATTTCAACCATGAAAAAGTATGTTGAGGCAATG
GGCGGGAAGCTCCGTATTGATGTAGAGCTTCCAACAGGAAAACATATAGGATTCAATGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3H713

Antitoxin

Source ID Structure
AlphaFold DB A0A0J2I7Z1

References