Detailed information of TA system TA228314

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 1964980..1965570
• Replicon: chromosome
• Accession: NZ_CP089448
• Organism: Klebsiella michiganensis strain 8-1

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: LT930_RS09345
• Protein ID: WP_182157821.1
• Coordinates: 1965238..1965570 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: -
• Locus tag: LT930_RS09340
• Protein ID: WP_064405693.1
• Coordinates: 1964980..1965237 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LT930_RS09315 (LT930_09315)	1960253..1960858	-	606	WP_047723405.1	glutathione S-transferase family protein	-
LT930_RS09320 (LT930_09320)	1961038..1961964	+	927	WP_014838892.1	LysR substrate-binding domain-containing protein	-
LT930_RS09325 (LT930_09325)	1962002..1963000	-	999	WP_048260911.1	aldo/keto reductase	-
LT930_RS09330 (LT930_09330)	1963101..1964015	+	915	WP_049084134.1	LysR family transcriptional regulator	-
LT930_RS09335 (LT930_09335)	1964154..1964270	-	117	Protein_1826	molybdopterin-binding protein	-
LT930_RS09340 (LT930_09340)	1964980..1965237	+	258	WP_064405693.1	hypothetical protein	Antitoxin
LT930_RS09345 (LT930_09345)	1965238..1965570	+	333	WP_182157821.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
LT930_RS09355 (LT930_09355)	1965893..1967350	+	1458	WP_025106072.1	EmmdR/YeeO family multidrug/toxin efflux MATE transporter	-
LT930_RS09365 (LT930_09365)	1968312..1969766	-	1455	WP_014229909.1	AMP nucleosidase	-
LT930_RS09370 (LT930_09370)	1969899..1970156	-	258	WP_014229908.1	histidine kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1955290..1965570	10280

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-109)

Antitoxin

• Antitoxin PemI-like (4-84)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11908.81 Da
• Isoelectric Point: 10.8850

>T228314 WP_182157821.1 NZ_CP089448:1965238-1965570 [Klebsiella michiganensis]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPITSGGNFARTAGFTVSLEGAGTKTTGVIRCDQPRTI
DMTARNGKRLERIPDAVVNEVLARLDAILR

Nucleotide

• Length: 333 bp

>T228314 NZ_CP123751:c1347289-1347065 [Ligilactobacillus animalis]
ATGGCGAATATTACTGACTTCACCGAAAAGCAATTTGAAGATCGTTTAGAAAAGAATGTTGAACGACTAACTAAAAATAG
ACTAGCGGTTGAATCGCCAACCGCTTTTTTACTTGGTGGGCAACCAGGGTCAGGGAAAACCAGTTTGCGATCAGCAATTT
TTGAAGAAACACAAGGGAATGTTATTGTGGTTCTGTTGCAAAGTTTTAAATCTACTATCAAATAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9452.97 Da
• Isoelectric Point: 4.4607

>AT228314 WP_064405693.1 NZ_CP089448:1964980-1965237 [Klebsiella michiganensis]
MYTTRLKKVGGSIMLAVPPAVLKTLELSMDSEVGMTINNGCLIIEPQKRPHYSLEELLAQCDPHAEMSEEDREWIDAPAV
GKEIL

Nucleotide

• Length: 258 bp

>AT228314 NZ_CP123751:c1347563-1347291 [Ligilactobacillus animalis]
ATGGCAGTTACGTATGAAAAAACATTTGAAATTGAAATCATTAATGAGTTATCGGCAAGTGTTTATAATCGGGTATTAAA
TTATGTTTTGAATCATGAACTAGATACTAAAAATACTCGTTTACTAGAAGTGAATCTTTTAAATCAATTAGAAGTGGCAC
AAGAAGTTGATTTATTTCAACAACCATTTGAAGAATTACAAGCTATTCATGAGTATTGGCGGTCAATGAATCAATATTCA
AAACAAATTTTGAATAAAGAGAAAGTGGCTTAA