Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 1964980..1965570 Replicon chromosome
Accession NZ_CP089448
Organism Klebsiella michiganensis strain 8-1

Toxin (Protein)


Gene name pemK Uniprot ID -
Locus tag LT930_RS09345 Protein ID WP_182157821.1
Coordinates 1965238..1965570 (+) Length 111 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID -
Locus tag LT930_RS09340 Protein ID WP_064405693.1
Coordinates 1964980..1965237 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LT930_RS09315 (LT930_09315) 1960253..1960858 - 606 WP_047723405.1 glutathione S-transferase family protein -
LT930_RS09320 (LT930_09320) 1961038..1961964 + 927 WP_014838892.1 LysR substrate-binding domain-containing protein -
LT930_RS09325 (LT930_09325) 1962002..1963000 - 999 WP_048260911.1 aldo/keto reductase -
LT930_RS09330 (LT930_09330) 1963101..1964015 + 915 WP_049084134.1 LysR family transcriptional regulator -
LT930_RS09335 (LT930_09335) 1964154..1964270 - 117 Protein_1826 molybdopterin-binding protein -
LT930_RS09340 (LT930_09340) 1964980..1965237 + 258 WP_064405693.1 hypothetical protein Antitoxin
LT930_RS09345 (LT930_09345) 1965238..1965570 + 333 WP_182157821.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
LT930_RS09355 (LT930_09355) 1965893..1967350 + 1458 WP_025106072.1 EmmdR/YeeO family multidrug/toxin efflux MATE transporter -
LT930_RS09365 (LT930_09365) 1968312..1969766 - 1455 WP_014229909.1 AMP nucleosidase -
LT930_RS09370 (LT930_09370) 1969899..1970156 - 258 WP_014229908.1 histidine kinase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 1955290..1965570 10280


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-109)

Antitoxin

Antitoxin PemI-like

(4-84)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 11908.81 Da        Isoelectric Point: 10.8850

>T228314 WP_182157821.1 NZ_CP089448:1965238-1965570 [Klebsiella michiganensis]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPITSGGNFARTAGFTVSLEGAGTKTTGVIRCDQPRTI
DMTARNGKRLERIPDAVVNEVLARLDAILR

Download         Length: 333 bp

>T228314 NZ_CP123751:c1347289-1347065 [Ligilactobacillus animalis]
ATGGCGAATATTACTGACTTCACCGAAAAGCAATTTGAAGATCGTTTAGAAAAGAATGTTGAACGACTAACTAAAAATAG
ACTAGCGGTTGAATCGCCAACCGCTTTTTTACTTGGTGGGCAACCAGGGTCAGGGAAAACCAGTTTGCGATCAGCAATTT
TTGAAGAAACACAAGGGAATGTTATTGTGGTTCTGTTGCAAAGTTTTAAATCTACTATCAAATAA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9452.97 Da        Isoelectric Point: 4.4607

>AT228314 WP_064405693.1 NZ_CP089448:1964980-1965237 [Klebsiella michiganensis]
MYTTRLKKVGGSIMLAVPPAVLKTLELSMDSEVGMTINNGCLIIEPQKRPHYSLEELLAQCDPHAEMSEEDREWIDAPAV
GKEIL

Download         Length: 258 bp

>AT228314 NZ_CP123751:c1347563-1347291 [Ligilactobacillus animalis]
ATGGCAGTTACGTATGAAAAAACATTTGAAATTGAAATCATTAATGAGTTATCGGCAAGTGTTTATAATCGGGTATTAAA
TTATGTTTTGAATCATGAACTAGATACTAAAAATACTCGTTTACTAGAAGTGAATCTTTTAAATCAATTAGAAGTGGCAC
AAGAAGTTGATTTATTTCAACAACCATTTGAAGAATTACAAGCTATTCATGAGTATTGGCGGTCAATGAATCAATATTCA
AAACAAATTTTGAATAAAGAGAAAGTGGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References