Detailed information of TA system TA228313

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1280479..1281262
• Replicon: chromosome
• Accession: NZ_CP089448
• Organism: Klebsiella michiganensis strain 8-1

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: LT930_RS06170
• Protein ID: WP_039077579.1
• Coordinates: 1280479..1280853 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: LT930_RS06175
• Protein ID: WP_039077578.1
• Coordinates: 1280903..1281262 (-)
• Length: 120 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LT930_RS06145 (LT930_06145)	1276161..1276394	-	234	WP_004132711.1	hypothetical protein	-
LT930_RS06150 (LT930_06150)	1276794..1277066	+	273	WP_016247220.1	hypothetical protein	-
LT930_RS06155 (LT930_06155)	1277269..1278177	+	909	WP_032938037.1	kdo(2)-lipid IV(A) palmitoleoyltransferase	-
LT930_RS06160 (LT930_06160)	1278951..1279940	+	990	WP_039077580.1	glycosyltransferase	-
LT930_RS06165 (LT930_06165)	1279944..1280402	+	459	WP_224232182.1	hypothetical protein	-
LT930_RS06170 (LT930_06170)	1280479..1280853	-	375	WP_039077579.1	TA system toxin CbtA family protein	Toxin
LT930_RS06175 (LT930_06175)	1280903..1281262	-	360	WP_039077578.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
LT930_RS06180 (LT930_06180)	1281285..1281506	-	222	WP_032938028.1	DUF987 domain-containing protein	-
LT930_RS06185 (LT930_06185)	1281520..1282002	-	483	WP_039077577.1	DNA repair protein RadC	-
LT930_RS06190 (LT930_06190)	1282243..1282722	-	480	WP_082027055.1	antirestriction protein	-
LT930_RS06195 (LT930_06195)	1282802..1283623	-	822	WP_039077576.1	DUF932 domain-containing protein	-
LT930_RS06200 (LT930_06200)	1283734..1283946	-	213	WP_032938024.1	hypothetical protein	-
LT930_RS06205 (LT930_06205)	1284056..1284529	-	474	WP_032938022.1	hypothetical protein	-
LT930_RS06210 (LT930_06210)	1284603..1285232	-	630	WP_039077575.1	hypothetical protein	-
LT930_RS06215 (LT930_06215)	1285330..1286007	-	678	WP_039077574.1	WYL domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1276897..1303975	27078

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (9-112)

Antitoxin

• Antitoxin CbeA (15-114)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13875.91 Da
• Isoelectric Point: 10.3681

>T228313 WP_039077579.1 NZ_CP089448:c1280853-1280479 [Klebsiella michiganensis]
MQKQSLSPHRAASSRLSSVEIWRKLLKYLLEQHYGLTLNDTPFGNDSVIQKHIDAGISLCYAVNFIVEKYDLVRTDRHGF
SVKEQSPFIGSIDMLRARKATGLMTRKGYKTVTDISGGRSSGGK

Nucleotide

• Length: 375 bp

>T228313 NZ_CP123735:c2132915-2132577 [Lactobacillus kefiranofaciens]
ATGGTAATTAAACAAGGATCCGTTTTATTGATGGATTTAAGTCCTAAGCAGGGACATGAACAATCCGGCAAGCGTCCATA
TTTATATTTAAGTTATCAAGGTGTTGAAAAATATGCCCATATAGCGATTTTTGCTCCAATTTCAACTACGAAACGGCGAT
ATCCACTATATAGAAATCTACCAGATTCTTGTAAAACTAAAGGCGTAGTTATGTTAGATCAACTTGTAACTGTGGATTAT
CATAATCGCAAACATCAATTTTTAGAGCAATTACCTGATGACTATATGGAAAAGTTGCTAAAGATTGTAAAGGTAGTATT
TCAAAAAGATCCTTCTTAA

Antitoxin

Protein

• Length: 120 a.a.
• Molecular weight: 13317.39 Da
• Isoelectric Point: 7.4144

>AT228313 WP_039077578.1 NZ_CP089448:c1281262-1280903 [Klebsiella michiganensis]
MQKATRVINHNIAEPWWGLRRNITPCFGARLVHEGNHLHYLADRASIAGTFNDADLRHLDQAFPVLMKQMELMLTSSELT
PHIQRCITLHAKGLICEADTLGSCGYLYIVIYPASATTA

Nucleotide

• Length: 360 bp

>AT228313 NZ_CP123735:c2133160-2132909 [Lactobacillus kefiranofaciens]
ATGATAACTGCTACAATTTCAAAATGGGGTAATTCTAAAGGTATTAGACTTAATCAGGCCGTTCTACAAGCATTAAATGC
AAAAACTGGTGATCATTTAAATTTTAAAATTGAGGATAATAGGGTAATTATCACTAAGAAGGAACCAAAACATGAACTTA
CTTTTGAAGAACTTTTTAAGGATTATAAAGGTGGTTCATTTGATACAAAGATTTCATTACCAGATGAAAGAGTGGGCAAT
GAAAAATGGTAA