Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 838742..839399 Replicon chromosome
Accession NZ_CP089448
Organism Klebsiella michiganensis strain 8-1

Toxin (Protein)


Gene name cptA Uniprot ID J5U333
Locus tag LT930_RS04040 Protein ID WP_004854060.1
Coordinates 838989..839399 (+) Length 137 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID H3N295
Locus tag LT930_RS04035 Protein ID WP_004124953.1
Coordinates 838742..839008 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LT930_RS04020 (LT930_04020) 833976..834401 - 426 WP_004854067.1 PTS sugar transporter subunit IIA -
LT930_RS04025 (LT930_04025) 834522..837320 - 2799 WP_049079129.1 transcriptional regulator DagR -
LT930_RS04030 (LT930_04030) 837514..838497 - 984 WP_014226793.1 tRNA-modifying protein YgfZ -
LT930_RS04035 (LT930_04035) 838742..839008 + 267 WP_004124953.1 FAD assembly factor SdhE Antitoxin
LT930_RS04040 (LT930_04040) 838989..839399 + 411 WP_004854060.1 protein YgfX Toxin
LT930_RS04045 (LT930_04045) 839408..839929 - 522 WP_014226792.1 flavodoxin FldB -
LT930_RS04050 (LT930_04050) 840051..840947 + 897 WP_004105555.1 site-specific tyrosine recombinase XerD -
LT930_RS04055 (LT930_04055) 840970..841683 + 714 WP_004854054.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
LT930_RS04060 (LT930_04060) 841689..843422 + 1734 WP_014226791.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 137 a.a.        Molecular weight: 16106.97 Da        Isoelectric Point: 10.9455

>T228312 WP_004854060.1 NZ_CP089448:838989-839399 [Klebsiella michiganensis]
VVLWQSDLRISWRAQWFSLLMHGVVAALVLLMPWPLSYTPLWLILLSLVVFDCVRSQRRIHARQGEIKLLIDSRLRWQKA
EWDIIGTPWVINSGMLLRLRNTENQRTQHLWVAADSMDAGEWRDLRRLVLLKPTQE

Download         Length: 411 bp

>T228312 NZ_CP123735:c1827477-1827214 [Lactobacillus kefiranofaciens]
ATGCTAAGTTGGACTGATGATGGCTGTGACGACTACTTGAATTGGCAAAAGCAAAGGCAGAAAAAGAAAATAAAACGTAT
CAACAAATTAATTACAGACATTAAACGTCATCCCTTTGAAGGAATGGGCAAACCGGAAGGCTTAAAGAATAATCTAACAG
GCTTATGGTCTCGTAAAATTGATGCTAAAAACAGAATTATTTATGAGTATACAAATGAGAACGTTATAATCTATTCCTGC
AAAGATCATTATGATGACCATTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10411.88 Da        Isoelectric Point: 5.1296

>AT228312 WP_004124953.1 NZ_CP089448:838742-839008 [Klebsiella michiganensis]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDTLSDGDKALFIRLLENDDPDLFNWLMNHGEPADAELQRMVKLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT228312 NZ_CP123735:c1827748-1827461 [Lactobacillus kefiranofaciens]
ATGGATGCTGTAAACTATAGTAATTTTAGAAAAGATTTAAAAACCTACTTTAAGCAAGTAAACGATGATAGTGAGCCTTT
GATTGTAACTAATAAAAATCCAGAAGATAATGTCGTTGTCTTAAGCAAAGATGAATATGACGCTTTAATAGAAACCTTGG
CAATTCAATCTAATAAATATCTAATGGATAAAATTAATCGTGGACGCAAGCAAGTAGCTAAAGCACAAACTAATCAACAT
GAACTAATTGATCCAGATGGCGAGGTATCAGATGCTAAGTTGGACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYA5

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3H3L9

References