Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-RelB
Location 642258..642850 Replicon chromosome
Accession NZ_CP089448
Organism Klebsiella michiganensis strain 8-1

Toxin (Protein)


Gene name doc Uniprot ID A0A7H4PHG5
Locus tag LT930_RS03120 Protein ID WP_004105955.1
Coordinates 642476..642850 (+) Length 125 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID A0A2J4YM08
Locus tag LT930_RS03115 Protein ID WP_004105957.1
Coordinates 642258..642479 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LT930_RS03095 (LT930_03095) 637815..638369 + 555 WP_064375019.1 YgjV family protein -
LT930_RS03100 (LT930_03100) 638388..639635 - 1248 WP_004105963.1 serine/threonine transporter SstT -
LT930_RS03105 (LT930_03105) 639890..640858 - 969 WP_048260487.1 TerC family protein -
LT930_RS03110 (LT930_03110) 641109..642098 - 990 WP_032693240.1 Gfo/Idh/MocA family oxidoreductase -
LT930_RS03115 (LT930_03115) 642258..642479 + 222 WP_004105957.1 type II toxin-antitoxin system prevent-host-death family antitoxin Antitoxin
LT930_RS03120 (LT930_03120) 642476..642850 + 375 WP_004105955.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
LT930_RS03125 (LT930_03125) 642830..643333 - 504 WP_025108311.1 M48 family metallopeptidase -
LT930_RS03130 (LT930_03130) 643412..645055 - 1644 WP_064375018.1 glycoside hydrolase family 43 protein -
LT930_RS03135 (LT930_03135) 645184..646050 - 867 WP_064375017.1 AraC family transcriptional regulator -
LT930_RS03140 (LT930_03140) 646158..647501 + 1344 WP_064375016.1 glycoside-pentoside-hexuronide (GPH):cation symporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(5-119)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-52)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13895.93 Da        Isoelectric Point: 6.0769

>T228311 WP_004105955.1 NZ_CP089448:642476-642850 [Klebsiella michiganensis]
MKWVSASEVIAFHDRILQHLPGVKGMSDPGRAEAIIYRVQNRFHYEGVNDIFELAATYWVAIARGHIFNDGNKRTAFFIT
MTFLARNGYLIADEDTRLEELTVLAATGEATVVVLADALRQLAL

Download         Length: 375 bp

>T228311 NZ_CP123735:495913-496182 [Lactobacillus kefiranofaciens]
ATGATTTTAAAATATCATGGCAGGATTAAAAACTCAGCTAAGCCTGATTTAAAGAAGATTAAGCGAAGTAATCTGAAAGA
TAATTTTCAAGATATTATAGATCAACTCAAAAAAGATCCTTATAAAAAGAATCAAGGATTTGAAAAATTAGAACCACCAA
TTTTAGGAAAATATTCTCGAAGAATCAATATAAAACATCGAGTTGTTTATACTGTTGATGATGTTAATAAGATAGTAAGT
ATTTACTCGGCCTGGTCACATTATGAATGA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8185.19 Da        Isoelectric Point: 5.7996

>AT228311 WP_004105957.1 NZ_CP089448:642258-642479 [Klebsiella michiganensis]
MRSYSTSEARQNIAAVMDAAASGEPIEITRRDGTSNVLISKAEYETFMNAKLDAEFDFIMQRHGRTVKALTDR

Download         Length: 222 bp

>AT228311 NZ_CP123735:495650-495916 [Lactobacillus kefiranofaciens]
ATGGATGCAACAATTTATACTCCCACAGGAGCAAGACGTAATTTATTCAATCTTATCAAGCAAGTTAATAAAAACCATAC
TCCAGTTAATATTGAATCTACAAAAGATAAAGCAGAAAGTGCTGTGTTAATAAGTAAATCCGACTGGGACGCTATTCAGG
AGACTCTTTATCTTGAGTCCAATGGTGTAGGAGAAGTTGTGAGAAAACGTGAAAAGGACAATTCAGGTTTTACTGATATT
GATGATATTAACTGGGATGAGCTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7H4PHG5

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4YM08

References