228310

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 56803..57350 Replicon chromosome
Accession NZ_CP089448
Organism Klebsiella michiganensis strain 8-1

Toxin (Protein)


Gene name ccdB Uniprot ID A0A0H3H5T3
Locus tag LT930_RS00285 Protein ID WP_014227299.1
Coordinates 56803..57111 (-) Length 103 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID A0A0H3H0P7
Locus tag LT930_RS00290 Protein ID WP_014227298.1
Coordinates 57114..57350 (-) Length 79 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LT930_RS00260 (LT930_00260) 52707..53018 - 312 WP_014227304.1 PTS sugar transporter subunit IIB -
LT930_RS00265 (LT930_00265) 53313..54254 + 942 WP_025107585.1 LacI family DNA-binding transcriptional regulator -
LT930_RS00270 (LT930_00270) 54278..54571 - 294 WP_064357598.1 YicS family protein -
LT930_RS00275 (LT930_00275) 54781..55746 + 966 WP_064357600.1 Rpn family recombination-promoting nuclease/putative transposase -
LT930_RS00280 (LT930_00280) 55750..56652 - 903 WP_014227300.1 EamA family transporter -
LT930_RS00285 (LT930_00285) 56803..57111 - 309 WP_014227299.1 CcdB family protein Toxin
LT930_RS00290 (LT930_00290) 57114..57350 - 237 WP_014227298.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
LT930_RS00295 (LT930_00295) 57456..58889 - 1434 WP_048262922.1 PTS N-acetylmuramic acid transporter subunit IIBC -
LT930_RS00300 (LT930_00300) 58914..59816 - 903 WP_009654080.1 N-acetylmuramic acid 6-phosphate etherase -
LT930_RS00305 (LT930_00305) 59977..61143 - 1167 WP_025107588.1 multidrug effflux MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-101)

Antitoxin

Post-segregation antitoxin CcdA

(8-78)


Sequences

Toxin        


Download         Length: 103 a.a.        Molecular weight: 11725.57 Da        Isoelectric Point: 7.2729

>T228310 WP_014227299.1 NZ_CP089448:c57111-56803 [Klebsiella michiganensis]
MQYYVYKNTGRIAAYPYLLDVQSDIIGKRNTRVVIPLFPLKNYKGPRADRLTPLVTVEGEEYVVMTHELASIPHRVLGEE
VCNLNHQREVVKASMDFLFDGI

Download         Length: 309 bp

>T228310 NZ_CP123661:98993-99271 [Salmonella enterica subsp. enterica serovar Kentucky]
ATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGATGGC
CGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCCTGGCCGGGCAGCCGTATAAAGGCCGCAACGGTCGTG
TGGAAGGTACCCGGGAACTGGTTATTCACCCGCATTTTGTTCTGGTTTATGAGGTCGACAGCCAGTGGGGAAAAGTGTAT
ATCCTGCGTGTATTGCATACTGCACAGAAGTGGCCATAG

Antitoxin


Download         Length: 79 a.a.        Molecular weight: 8783.92 Da        Isoelectric Point: 5.7928

>AT228310 WP_014227298.1 NZ_CP089448:c57350-57114 [Klebsiella michiganensis]
MPAAKRNKQNVSVTLDPGLVKQAREVGINLSLTLTNALKSEIHSLEIARWQKENAQAIDYLNNLTEENGLLSDEYRTF

Download         Length: 237 bp

>AT228310 NZ_CP123661:98724-98993 [Salmonella enterica subsp. enterica serovar Kentucky]
ATGGCACAGGTTAATATGAGTTTAAGAATTGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATCCGGGATTTTATGCGCCAGACCGTGGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
AGGCAGGACGTCAGCAACTCGAGCGCGGCGATGTGCTTCCCCATGACATGGTCGAATCTTCCGCTGCTGCATGGCGGGAT
GAAATGAGCAGGAAAGTCGCCGGTAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3H5T3

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3H0P7

References