Detailed information of TA system TA022829

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 3522044..3522675
• Replicon: chromosome
• Accession: NC_010602
• Organism: Leptospira biflexa serovar Patoc strain Patoc 1 (Paris)

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: B0SRH1
• Locus tag: LEPBI_RS16660
• Protein ID: WP_012390319.1
• Coordinates: 3522044..3522448 (-)
• Length: 135 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: B0SRH2
• Locus tag: LEPBI_RS16665
• Protein ID: WP_012390320.1
• Coordinates: 3522445..3522675 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LEPBI_RS16630	3517834..3518109	-	276	WP_012390313.1	thioesterase	-
LEPBI_RS16635	3518169..3518636	-	468	WP_012390314.1	hypothetical protein	-
LEPBI_RS16640	3518654..3518989	-	336	WP_012476462.1	OB-fold domain-containing protein	-
LEPBI_RS16645	3519057..3519437	-	381	WP_012390316.1	nuclear transport factor 2 family protein	-
LEPBI_RS16650	3519487..3520686	-	1200	WP_012390317.1	SH3 domain-containing protein	-
LEPBI_RS16655	3520870..3521850	-	981	WP_041770094.1	BtrH N-terminal domain-containing protein	-
LEPBI_RS16660	3522044..3522448	-	405	WP_012390319.1	type II toxin-antitoxin system VapC family toxin	Toxin
LEPBI_RS16665	3522445..3522675	-	231	WP_012390320.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
LEPBI_RS16670	3523058..3524272	-	1215	WP_012390321.1	acetyl-CoA C-acetyltransferase	-
LEPBI_RS16675	3524344..3525417	-	1074	WP_012390322.1	hypothetical protein	-
LEPBI_RS16680	3525465..3525992	-	528	WP_012390323.1	NADH-quinone oxidoreductase subunit I	-
LEPBI_RS16685	3525979..3527427	-	1449	WP_012390324.1	(2Fe-2S)-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-124)

Antitoxin

• SpoVT-AbrB domain (7-48)

Sequences

Toxin

Protein

• Length: 135 a.a.
• Molecular weight: 15507.87 Da
• Isoelectric Point: 8.7364

>T22829 WP_012390319.1 NC_010602:c3522448-3522044 [Leptospira biflexa serovar Patoc strain 'Patoc 1 (Paris)']
MNHYLLDTNICIYIINKKPENVYQKFKKISLDNIFISSITEFELRYGVQKSQKADKNQKTLNEFLGYLNVINFDSESASV
AGTIRTKLEKKGEIIGPYDLLIASQAIASDIILVTNNEKEYKRIKELKIENWIN

Nucleotide

• Length: 405 bp

>T22829 NC_010602:c3522448-3522044 [Leptospira biflexa serovar Patoc strain 'Patoc 1 (Paris)']
ATGAATCATTATTTATTAGATACAAATATTTGTATTTATATCATTAATAAAAAACCTGAAAATGTTTACCAAAAATTCAA
AAAAATTAGTTTAGATAACATCTTCATTTCTAGCATCACTGAATTTGAATTAAGATATGGTGTTCAAAAAAGTCAAAAAG
CTGATAAAAATCAAAAAACTCTAAATGAATTTCTGGGTTACCTAAATGTCATAAACTTTGACTCAGAATCAGCATCGGTA
GCAGGAACTATCAGAACTAAACTAGAGAAAAAGGGAGAAATAATTGGACCGTATGATTTACTCATCGCTTCTCAGGCTAT
TGCTAGTGACATTATTTTAGTTACAAATAATGAAAAAGAGTATAAAAGAATTAAAGAACTTAAAATAGAAAACTGGATTA
ATTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8935.20 Da
• Isoelectric Point: 10.1006

>AT22829 WP_012390320.1 NC_010602:c3522675-3522445 [Leptospira biflexa serovar Patoc strain 'Patoc 1 (Paris)']
MNRAKLFKNGDSQAVRLPKEFRFKGKEVYIRKDGNCVIISPIDDAVDRLWKSLNDFSDDFLIERNQPKSFDKRNSI

Nucleotide

• Length: 231 bp

>AT22829 NC_010602:c3522675-3522445 [Leptospira biflexa serovar Patoc strain 'Patoc 1 (Paris)']
ATGAATCGTGCGAAATTATTTAAAAATGGTGATAGTCAAGCGGTTAGACTCCCTAAAGAATTTAGATTTAAAGGAAAAGA
AGTCTACATTAGAAAAGATGGAAATTGCGTTATTATATCACCTATCGACGATGCCGTTGACAGATTATGGAAGTCTCTAA
ATGATTTTTCGGATGATTTTCTCATTGAGAGAAATCAACCTAAGTCTTTCGACAAACGAAATTCTATATGA

Structures

Toxin

• AlphaFold DB: B0SRH1

Antitoxin

• AlphaFold DB: B0SRH2