Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 2348417..2350005 Replicon chromosome
Accession NZ_CP089445
Organism Escherichia coli strain 5-1

Toxin (Protein)


Gene name hipA Uniprot ID A0A061YM43
Locus tag LT922_RS11500 Protein ID WP_001125411.1
Coordinates 2348417..2349739 (-) Length 441 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A061YNW4
Locus tag LT922_RS11505 Protein ID WP_001296076.1
Coordinates 2349739..2350005 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LT922_RS11480 (2343698) 2343698..2344201 - 504 WP_000825466.1 fimbrial protein -
LT922_RS11485 (2344214) 2344214..2344744 - 531 WP_000876789.1 fimbrial protein -
LT922_RS11490 (2344758) 2344758..2347409 - 2652 WP_001531687.1 fimbria/pilus outer membrane usher protein -
LT922_RS11495 (2347451) 2347451..2347882 - 432 Protein_2245 fimbria/pilus periplasmic chaperone -
LT922_RS11500 (2348417) 2348417..2349739 - 1323 WP_001125411.1 type II toxin-antitoxin system serine/threonine protein kinase toxin HipA Toxin
LT922_RS11505 (2349739) 2349739..2350005 - 267 WP_001296076.1 type II toxin-antitoxin system antitoxin HipB Antitoxin
LT922_RS11510 (2350313) 2350313..2351071 + 759 WP_001286537.1 trans-aconitate 2-methyltransferase -
LT922_RS11515 (2351075) 2351075..2351989 - 915 WP_001296077.1 bestrophin family protein -
LT922_RS11520 (2352186) 2352186..2353637 - 1452 WP_000854638.1 tagaturonate reductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 2334609..2350005 15396


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(7-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(18-69)


Sequences

Toxin        


Download         Length: 441 a.a.        Molecular weight: 49329.96 Da        Isoelectric Point: 7.7906

>T228286 WP_001125411.1 NZ_CP089445:c2349739-2348417 [Escherichia coli]
MPKLVTWMNNQRVGELTKLANGAHTFKYAAEWLASRYARPLSLSLPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLIPEDETVTCPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGRVRALAVERFDRRW
NTERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIAQIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLKFPEVQMHEILSDFARMIP
AALDNVKTSLPTDFPENVVTAVETNVLRLHDRLSREYGIK

Download         Length: 1323 bp

>T228286 NZ_CP123642:638060-638162 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9987.34 Da        Isoelectric Point: 4.9632

>AT228286 WP_001296076.1 NZ_CP089445:c2350005-2349739 [Escherichia coli]
MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTSLTTFFKILQSLELSMTLCDAKNASPEA
TEQQDLEW

Download         Length: 267 bp

>AT228286 NZ_CP123642:c638169-638024 [Klebsiella pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A061YM43

Antitoxin

Source ID Structure
AlphaFold DB A0A061YNW4

References