Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1253841..1254459 Replicon chromosome
Accession NZ_CP089445
Organism Escherichia coli strain 5-1

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag LT922_RS06030 Protein ID WP_001291435.1
Coordinates 1253841..1254059 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag LT922_RS06035 Protein ID WP_000344800.1
Coordinates 1254085..1254459 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LT922_RS05995 (1249128) 1249128..1249700 + 573 WP_000779831.1 YbaY family lipoprotein -
LT922_RS06000 (1249731) 1249731..1250042 - 312 WP_000409908.1 MGMT family protein -
LT922_RS06010 (1250421) 1250421..1250774 + 354 WP_000878135.1 DUF1428 family protein -
LT922_RS06015 (1250816) 1250816..1252366 - 1551 WP_001385227.1 cyclic-guanylate-specific phosphodiesterase PdeB -
LT922_RS06020 (1252530) 1252530..1253000 - 471 WP_000136192.1 YlaC family protein -
LT922_RS06025 (1253116) 1253116..1253667 - 552 WP_000102539.1 maltose O-acetyltransferase -
LT922_RS06030 (1253841) 1253841..1254059 - 219 WP_001291435.1 HHA domain-containing protein Toxin
LT922_RS06035 (1254085) 1254085..1254459 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
LT922_RS06040 (1255005) 1255005..1258154 - 3150 WP_001132478.1 efflux RND transporter permease AcrB -
LT922_RS06045 (1258177) 1258177..1259370 - 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T228280 WP_001291435.1 NZ_CP089445:c1254059-1253841 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T228280 NZ_CP089445:c1254059-1253841 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT228280 WP_000344800.1 NZ_CP089445:c1254459-1254085 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT228280 NZ_CP089445:c1254459-1254085 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References