Detailed information of TA system TA022828

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): chpIK/PemK(toxin)
• Location: 3177800..3178379
• Replicon: chromosome
• Accession: NC_010602
• Organism: Leptospira biflexa serovar Patoc strain Patoc 1 (Paris)

Toxin (Protein)

• Gene name: chpK
• Uniprot ID: B0SPM6
• Locus tag: LEPBI_RS15010
• Protein ID: WP_012389990.1
• Coordinates: 3177800..3178144 (-)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: chpI
• Uniprot ID: B0SPM7
• Locus tag: LEPBI_RS15015
• Protein ID: WP_012389991.1
• Coordinates: 3178131..3178379 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LEPBI_RS14990	3173480..3174664	+	1185	WP_012389985.1	hypothetical protein	-
LEPBI_RS14995	3175118..3175816	+	699	WP_012389987.1	class I SAM-dependent methyltransferase	-
LEPBI_RS15000	3176156..3176725	-	570	WP_012389988.1	hypothetical protein	-
LEPBI_RS15005	3176796..3177728	-	933	WP_012389989.1	hypothetical protein	-
LEPBI_RS15010	3177800..3178144	-	345	WP_012389990.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
LEPBI_RS15015	3178131..3178379	-	249	WP_012389991.1	hypothetical protein	Antitoxin
LEPBI_RS15020	3178571..3179458	-	888	WP_012389992.1	ankyrin repeat domain-containing protein	-
LEPBI_RS15025	3179841..3180662	-	822	WP_012389993.1	ankyrin repeat domain-containing protein	-
LEPBI_RS15030	3180787..3181590	-	804	WP_012389994.1	hypothetical protein	-
LEPBI_RS19160	3181607..3181783	-	177	WP_167530803.1	hypothetical protein	-
LEPBI_RS15040	3182149..3182787	+	639	WP_012389996.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-112)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 12853.94 Da
• Isoelectric Point: 9.8443

>T22828 WP_012389990.1 NC_010602:c3178144-3177800 [Leptospira biflexa serovar Patoc strain 'Patoc 1 (Paris)']
MTRGEIWWVDLGIPFGSEPGFQRPVLIVQNNAFNHSNINTIIVVPLTTNLHLATAPGNSMLKKEDTNLSKDSIVNVSQIV
TIDRERFIKKVTEIKNKHMKKVEEGMKLVLSLES

Nucleotide

• Length: 345 bp

>T22828 NC_010602:c3178144-3177800 [Leptospira biflexa serovar Patoc strain 'Patoc 1 (Paris)']
ATGACACGTGGTGAAATCTGGTGGGTTGACCTTGGGATTCCATTTGGAAGTGAACCTGGATTCCAAAGACCAGTTTTAAT
CGTTCAAAATAATGCCTTTAATCATAGTAATATTAACACCATCATCGTTGTTCCATTGACCACGAACTTGCATTTAGCAA
CGGCACCAGGCAATTCAATGTTAAAAAAGGAAGATACAAATCTTTCCAAAGATTCAATTGTCAATGTCTCACAAATTGTA
ACCATTGATCGAGAACGCTTCATCAAAAAGGTAACAGAAATTAAAAACAAACATATGAAAAAAGTGGAAGAAGGGATGAA
ATTAGTTCTTTCTTTGGAATCTTGA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9116.35 Da
• Isoelectric Point: 8.6015

>AT22828 WP_012389991.1 NC_010602:c3178379-3178131 [Leptospira biflexa serovar Patoc strain 'Patoc 1 (Paris)']
MKTAVSIPDDLFVSAEKTAKKLGIPRSQLFALALEEFIQNHSNESITDSLNKVYTKSTKETETLASSLSVATLRRSLKND
TW

Nucleotide

• Length: 249 bp

>AT22828 NC_010602:c3178379-3178131 [Leptospira biflexa serovar Patoc strain 'Patoc 1 (Paris)']
ATGAAAACTGCGGTATCAATTCCAGACGATCTTTTTGTATCTGCGGAAAAAACAGCAAAAAAATTAGGTATTCCCAGAAG
TCAGTTGTTTGCACTCGCGCTTGAAGAATTCATTCAAAACCATTCCAATGAAAGCATAACAGATAGTTTAAACAAAGTGT
ATACAAAATCGACAAAAGAAACAGAAACTTTGGCATCGAGTCTCTCTGTCGCCACCTTACGAAGAAGTCTAAAAAATGAC
ACGTGGTGA

Structures

Toxin

• AlphaFold DB: B0SPM6

Antitoxin

• AlphaFold DB: B0SPM7