Detailed information of TA system
Bioinformatically predictedOverview
TA module
Type | II | Classification (family/domain) | shpAB/BrnT-BrnA |
Location | 692985..693561 | Replicon | chromosome |
Accession | NZ_CP089445 | ||
Organism | Escherichia coli strain 5-1 |
Toxin (Protein)
Gene name | shpA | Uniprot ID | A0A061KXE4 |
Locus tag | LT922_RS03340 | Protein ID | WP_001295743.1 |
Coordinates | 692985..693272 (+) | Length | 96 a.a. |
Antitoxin (Protein)
Gene name | shpB | Uniprot ID | A0A066STF6 |
Locus tag | LT922_RS03345 | Protein ID | WP_000063148.1 |
Coordinates | 693259..693561 (+) | Length | 101 a.a. |
Genomic Context
Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
---|---|---|---|---|---|---|
LT922_RS03325 (688645) | 688645..689949 | - | 1305 | WP_000535012.1 | restriction endonuclease subunit S | - |
LT922_RS03330 (689939) | 689939..691558 | - | 1620 | WP_001029745.1 | class I SAM-dependent DNA methyltransferase | - |
LT922_RS03335 (691622) | 691622..692788 | - | 1167 | WP_000800831.1 | restriction endonuclease | - |
LT922_RS03340 (692985) | 692985..693272 | + | 288 | WP_001295743.1 | BrnT family toxin | Toxin |
LT922_RS03345 (693259) | 693259..693561 | + | 303 | WP_000063148.1 | BrnA antitoxin family protein | Antitoxin |
LT922_RS03350 (693595) | 693595..694551 | - | 957 | WP_001295745.1 | GTPase | - |
LT922_RS03355 (694562) | 694562..694765 | - | 204 | WP_000467859.1 | YbdD/YjiX family protein | - |
LT922_RS03360 (694815) | 694815..696965 | - | 2151 | WP_001295524.1 | pyruvate/proton symporter BtsT | - |
Associated MGEs
MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 96 a.a. Molecular weight: 11156.62 Da Isoelectric Point: 7.4697
>T228277 WP_001295743.1 NZ_CP089445:692985-693272 [Escherichia coli]
MPMEFEWDANKAKSNRVKHGIRFEDAVLVFDDPQHLSQQDRIENGEYRWQTIGLVHGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERSRYEHG
MPMEFEWDANKAKSNRVKHGIRFEDAVLVFDDPQHLSQQDRIENGEYRWQTIGLVHGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERSRYEHG
Download Length: 288 bp
>T228277 NZ_CP123607:c2883995-2883899 [Serratia marcescens]
AACAAGCCCTGCATTAAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTAGACCGAATATAGGAATC
GTATTCGGTCTTTTTTT
AACAAGCCCTGCATTAAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTAGACCGAATATAGGAATC
GTATTCGGTCTTTTTTT
Antitoxin
Download Length: 101 a.a. Molecular weight: 11592.12 Da Isoelectric Point: 9.4646
>AT228277 WP_000063148.1 NZ_CP089445:693259-693561 [Escherichia coli]
MSMVKHKRGTPSTLDVQHEAELKALANKFDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK
MSMVKHKRGTPSTLDVQHEAELKALANKFDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK
Download Length: 303 bp
>AT228277 NZ_CP123607:2883899-2884039 [Serratia marcescens]
AAAAAAAGACCGAATACGATTCCTATATTCGGTCTAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCAT
TTAATGCAGGGCTTGTTCAGCCGTGCACTTTAAGAGTAGCCTACCGCGCCAGTTTTGCCAG
AAAAAAAGACCGAATACGATTCCTATATTCGGTCTAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCAT
TTAATGCAGGGCTTGTTCAGCCGTGCACTTTAAGAGTAGCCTACCGCGCCAGTTTTGCCAG
Similar Proteins
Only experimentally validated proteins are listed.
Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
---|
Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
---|
Structures
Toxin
Source | ID | Structure |
---|---|---|
AlphaFold DB | A0A061KXE4 |
Antitoxin
Source | ID | Structure |
---|---|---|
AlphaFold DB | A0A066STF6 |