Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 692985..693561 Replicon chromosome
Accession NZ_CP089445
Organism Escherichia coli strain 5-1

Toxin (Protein)


Gene name shpA Uniprot ID A0A061KXE4
Locus tag LT922_RS03340 Protein ID WP_001295743.1
Coordinates 692985..693272 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A066STF6
Locus tag LT922_RS03345 Protein ID WP_000063148.1
Coordinates 693259..693561 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LT922_RS03325 (688645) 688645..689949 - 1305 WP_000535012.1 restriction endonuclease subunit S -
LT922_RS03330 (689939) 689939..691558 - 1620 WP_001029745.1 class I SAM-dependent DNA methyltransferase -
LT922_RS03335 (691622) 691622..692788 - 1167 WP_000800831.1 restriction endonuclease -
LT922_RS03340 (692985) 692985..693272 + 288 WP_001295743.1 BrnT family toxin Toxin
LT922_RS03345 (693259) 693259..693561 + 303 WP_000063148.1 BrnA antitoxin family protein Antitoxin
LT922_RS03350 (693595) 693595..694551 - 957 WP_001295745.1 GTPase -
LT922_RS03355 (694562) 694562..694765 - 204 WP_000467859.1 YbdD/YjiX family protein -
LT922_RS03360 (694815) 694815..696965 - 2151 WP_001295524.1 pyruvate/proton symporter BtsT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11156.62 Da        Isoelectric Point: 7.4697

>T228277 WP_001295743.1 NZ_CP089445:692985-693272 [Escherichia coli]
MPMEFEWDANKAKSNRVKHGIRFEDAVLVFDDPQHLSQQDRIENGEYRWQTIGLVHGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERSRYEHG

Download         Length: 288 bp

>T228277 NZ_CP123607:c2883995-2883899 [Serratia marcescens]
AACAAGCCCTGCATTAAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTAGACCGAATATAGGAATC
GTATTCGGTCTTTTTTT

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11592.12 Da        Isoelectric Point: 9.4646

>AT228277 WP_000063148.1 NZ_CP089445:693259-693561 [Escherichia coli]
MSMVKHKRGTPSTLDVQHEAELKALANKFDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT228277 NZ_CP123607:2883899-2884039 [Serratia marcescens]
AAAAAAAGACCGAATACGATTCCTATATTCGGTCTAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCAT
TTAATGCAGGGCTTGTTCAGCCGTGCACTTTAAGAGTAGCCTACCGCGCCAGTTTTGCCAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A061KXE4

Antitoxin

Source ID Structure
AlphaFold DB A0A066STF6

References