Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/ElaA-DUF1778
Location 70441..71180 Replicon plasmid pHNLW22-1
Accession NZ_CP089442
Organism Klebsiella quasipneumoniae strain GLW9C22

Toxin (Protein)


Gene name tacT Uniprot ID A0A5E1AVR5
Locus tag LT990_RS25015 Protein ID WP_013087172.1
Coordinates 70441..70926 (-) Length 162 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID A0A5E1AWX8
Locus tag LT990_RS25020 Protein ID WP_012540086.1
Coordinates 70914..71180 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LT990_RS25000 (LT990_24995) 65920..66468 + 549 WP_023320124.1 GNAT family N-acetyltransferase -
LT990_RS25005 (LT990_25000) 66660..68396 + 1737 WP_064175054.1 LA2681 family HEPN domain-containing protein -
LT990_RS25010 (LT990_25005) 68503..69516 - 1014 WP_227545128.1 HNH endonuclease signature motif containing protein -
LT990_RS25015 (LT990_25010) 70441..70926 - 486 WP_013087172.1 GNAT family N-acetyltransferase Toxin
LT990_RS25020 (LT990_25015) 70914..71180 - 267 WP_012540086.1 DUF1778 domain-containing protein Antitoxin
LT990_RS25025 (LT990_25020) 71480..71803 + 324 WP_012540250.1 metalloregulator ArsR/SmtB family transcription factor -
LT990_RS25030 (LT990_25025) 71805..72518 + 714 WP_012540184.1 arsenical resistance protein ArsH -
LT990_RS25035 (LT990_25030) 72527..73072 + 546 WP_064175019.1 sigma-70 family RNA polymerase sigma factor -
LT990_RS25040 (LT990_25035) 73148..73509 + 362 Protein_71 arsenic metallochaperone ArsD family protein -
LT990_RS25045 (LT990_25040) 73530..75287 + 1758 WP_135720568.1 arsenical pump-driving ATPase -
LT990_RS25050 (LT990_25045) 75412..75948 + 537 WP_012540238.1 GNAT family N-acetyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid floR / sul2 / aph(4)-Ia / aac(3)-IVa / aph(3')-Ia / aadA2 / dfrA12 / tet(A) pla 1..259761 259761
- flank IS/Tn - - 65017..65520 503


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(27-152)

Antitoxin

Vibrio phage ICP1, Orf50

(2-87)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17810.62 Da        Isoelectric Point: 9.8560

>T228270 WP_013087172.1 NZ_CP089442:c70926-70441 [Klebsiella quasipneumoniae]
VGRVTVPEPLSSFHQVAEFVCGETVLDDWLKQKGLKNQALGSARTFVVCKKDTKQVAGFYSLATGSVNHTEATGNLRRNM
PDPIPVIILARLAVDLSFRGKGLGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKNFYIHHGFKPSQTQQRTLFLKLP
Q

Download         Length: 486 bp

>T228270 NZ_CP123599:44032-45357 [Enterobacter cloacae]
ATGAGCCGGAAACAGCAGCGTCTGGCAATCTGGATGAACGGAATCAAAGTCGGATACTGGGAAAAAAGTAAAGGGGTAGA
CAGCTTACAATACCTTCCTGACTGGAGTGCAGACGAACAGGGAAGGCCGCTGTCATTGTCCCTTCCTTTTACTCCCGGGA
ACCAGGTCTGGCGTGGAAATGTGGTACGTGACTATTTTGATAATTTACTCCCGGACAGCGAAGGCATACGCAGACGTCTG
GCAATGCGTTACAAGGCCGATAGCCTGGAGCCTTTTGATCTGCTGACGGAGCTGGGAAAAGACTGCGTGGGCGCGATACA
GCTGCTTCATGACGGAGACGAACCCACAGATTTATATTCCGTGAAGTATCACCCTCTTACTGAATCAGAAATTGCGGCAA
CTTTGCGTAATACCACGGAGACATTGCTACCGGGCAGGCCAGAAGATAACGACGACTTACGTTTATCTATTGCCGGTGCT
CAGGAGAAAACAGCCCTACTGTGGCATGATGATCGGTGGTGCATGCCGGAGGGTAATACACCAACGACGCATATATTCAA
GCTACCGCTCGGACTTGTGGGGAACATGAAAGCCGACATGAGCTCGTCGGTTGAAAATGAATGGCTCTGCTCGGTACTTC
TCGACCAGTACGGGCTCCCCGTGGCAAGAACGCAGATTGCTCACTTTGAAGATCAGAAAGCGCTGGTGGTAGAACGTTTT
GACCGAAAATGGTCAGGAGACGGACAATGGATCATTCGTTTACCCCAGGAGGATATGTGTCAGGCCCTGGGCGTTTCGCC
ATTACGAAAATATCAGGCGGACGGCGGGCCAGGTATATCGGAAATTATGGAGGTTCTGAGTAATTCAGACCGTGCTGAGC
GTGACAAAGCGCAGTTTTTTATGACGCAGATAATTTTCTGGATGATGGCCGCAACAGATGGCCACGCTAAAAATTTCAGT
ATTTCTATTGGGCCACAGGGACGTTATCACCTTACACCAAACTATGATGTGTTGTCGGCATGGCCGGTGATCGGTCATGG
GAACAATCAGATATCCTGGCAGAAGTGCAAACTGGCGATGGCCGTACGTGGCAGTAGCAACTATTACCAGATATATCGAA
TACAACGACGCCACTGGATCAGGCATGGTGAGATAACCGGCCTGAGTAAACAGCAGACAGAGGAGATGATAGAGGAAATC
ATCGCCAGAACCCCGGGTGTCATTGAACGTGTTAGCGGGCTTCTTCCGGACCAGTTCCCCCAGCAGCTTGCGGAAAGTAT
TTTTGATGGAATGAGGCAGCAATGCAGGCGCCTGGCTGAGAAATGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10076.47 Da        Isoelectric Point: 4.3721

>AT228270 WP_012540086.1 NZ_CP089442:c71180-70914 [Klebsiella quasipneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACQAAENVILDRRVFNFNDEQYAEFIDMLDAPVADDPAIDKLL
ARKPQWDV

Download         Length: 267 bp

>AT228270 NZ_CP123599:43734-44027 [Enterobacter cloacae]
ATCAACAATGATTACCCGTTAAACACGCTCAACCAGTTGCGTCCCTTGCTCATTGGTTTTCGCAAGGCGAATGGCCTGAC
GCAAAAAGACCTGTCCGAAAGGTTAGGGGTCACTCAACAGACATACTCACGTCTGGAAGCCAACCCTGCCAGTGCGAGCA
TTGAACGGCTATTCAAGGTGTTTTCCATCCTGGGTGTAAAAATCAGCTTCTCCTCGACAACCACCTCTTCAGAGAGGAAG
CAGACGGAAGAAATGCTTAAATCAAACTCACCTGCACGACAGGAGAAATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5E1AVR5

Antitoxin

Source ID Structure
AlphaFold DB A0A5E1AWX8

References