Detailed information of TA system TA228269

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 30849..31492
• Replicon: plasmid pHNLW22-1
• Accession: NZ_CP089442
• Organism: Klebsiella quasipneumoniae strain GLW9C22

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: LT990_RS24845
• Protein ID: WP_016946796.1
• Coordinates: 30849..31265 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q84A07
• Locus tag: LT990_RS24850
• Protein ID: WP_001261276.1
• Coordinates: 31262..31492 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LT990_RS24835 (LT990_24830)	26946..28043	+	1098	WP_049015454.1	RepB family plasmid replication initiator protein	-
LT990_RS24840 (LT990_24835)	29283..30403	+	1121	WP_088765928.1	IS3 family transposase	-
LT990_RS24845 (LT990_24840)	30849..31265	-	417	WP_016946796.1	type II toxin-antitoxin system VapC family toxin	Toxin
LT990_RS24850 (LT990_24845)	31262..31492	-	231	WP_001261276.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
LT990_RS24855 (LT990_24850)	31811..32971	+	1161	WP_064171855.1	IS30-like element ISCfr4 family transposase	-
LT990_RS24860 (LT990_24855)	33291..35486	-	2196	WP_250681192.1	DEAD/DEAH box helicase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	floR / sul2 / aph(4)-Ia / aac(3)-IVa / aph(3')-Ia / aadA2 / dfrA12 / tet(A)	pla	1..259761	259761
	-	inside	IScluster/Tn	-	-	19180..32971	13791

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15064.56 Da
• Isoelectric Point: 8.5403

>T228269 WP_016946796.1 NZ_CP089442:c31265-30849 [Klebsiella quasipneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVK

Nucleotide

• Length: 417 bp

>T228269 NZ_CP123598:c4671925-4671440 [Enterobacter cloacae]
GTGGGACGTGTAACAGCACCAGAACCATTATCCAGCTCCCATCAACTAGCAGAGTTCGTCAGTGGTGAGGCAGTACTTGA
TGACTGGCTTAAACAGAGGGGATTAAAAAACCAAGCTCTTGGTGCAGCACGAACGTTCGTCGTTTGTAAAAGAGACACTA
AACAGGTTGTCGGTTTTTACTCTCTGGCCACCGGCAGCGTTAACCACGTGGAAGCAATGGGAAGCCTCAGGCGCAATATG
CCAGATCCAATTCCCGTAATCATACTTGCTCGCCTGGCAGTTGATGTTTCATACCGTGGCCAAGGGCTCGGGGCCGATTT
ACTGCATGATGCAGTTCTGCGCTGTTACCGTGTAGCTGAGAATATTGGAGTCCGTGCGATTATGGTTCATGCACTGACTG
AAGAAGCCAAAAACTTTTATCTTCACCATGGCTTTAAGGCATCACAAACTCAGGAACGGACATTGTTTTTAAAGTTACCA
CAGTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8721.74 Da
• Isoelectric Point: 4.3533

>AT228269 WP_001261276.1 NZ_CP089442:c31492-31262 [Klebsiella quasipneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT228269 NZ_CP123598:c4672179-4671913 [Enterobacter cloacae]
ATGAAATCAGATGTTCAGCTCAACCTTAGAGCTAAAGAATCTCAAAGAGCACTTATTGATGCTGCAGCAGAGATTCTCCA
TAAATCACGCACGGACTTCATCCTGGAAATGGCATGTCAGGCTGCGGAGAATGTGATCCTCGATCGTCGCGTATTTAATT
TTAACGATGAGCAGTATGCAGAGTTTATCGATATGCTCGATGCACCGGTTTCGGATGAGGCGGCTATTGATAAACTTCTG
GCAAGGAAACCTCAGTGGGACGTGTAA

Structures

Antitoxin

• AlphaFold DB: A0A387K023