Detailed information of TA system TA228268

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 5967..6877
• Replicon: plasmid pHNLW22-1
• Accession: NZ_CP089442
• Organism: Klebsiella quasipneumoniae strain GLW9C22

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: LT990_RS24730
• Protein ID: WP_152135257.1
• Coordinates: 6407..6877 (+)
• Length: 157 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A7H4PRG4
• Locus tag: LT990_RS24725
• Protein ID: WP_032413443.1
• Coordinates: 5967..6410 (+)
• Length: 148 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LT990_RS24690 (LT990_24685)	1833..2572	-	740	Protein_1	tyrosine-type recombinase/integrase	-
LT990_RS24695 (LT990_24690)	2675..2815	-	141	Protein_2	hypothetical protein	-
LT990_RS24700 (LT990_24695)	2922..3195	+	274	Protein_3	IS3 family transposase	-
LT990_RS24705 (LT990_24700)	3333..3869	-	537	WP_023288364.1	hypothetical protein	-
LT990_RS24710 (LT990_24705)	4080..5048	-	969	WP_250681187.1	IS5-like element IS903B family transposase	-
LT990_RS24715 (LT990_24710)	5068..5253	+	186	WP_104158587.1	DUF2188 domain-containing protein	-
LT990_RS24720 (LT990_24715)	5355..5867	+	513	Protein_7	DUF4113 domain-containing protein	-
LT990_RS24725 (LT990_24720)	5967..6410	+	444	WP_032413443.1	DUF2384 domain-containing protein	Antitoxin
LT990_RS24730 (LT990_24725)	6407..6877	+	471	WP_152135257.1	RES family NAD+ phosphorylase	Toxin
LT990_RS24735 (LT990_24730)	6987..7247	-	261	WP_017901409.1	hypothetical protein	-
LT990_RS24740 (LT990_24735)	7947..9316	+	1370	WP_085956500.1	IS3-like element ISKpn8 family transposase	-
LT990_RS24745 (LT990_24740)	9335..9643	-	309	Protein_12	integrase core domain-containing protein	-
LT990_RS24750 (LT990_24745)	9750..10718	+	969	WP_250681188.1	IS5 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	floR / sul2 / aph(4)-Ia / aac(3)-IVa / aph(3')-Ia / aadA2 / dfrA12 / tet(A)	pla	1..259761	259761
	-	inside	IScluster/Tn	-	-	4080..10718	6638

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-148)

Antitoxin

• Antitoxin Xre (16-147)

Sequences

Toxin

Protein

• Length: 157 a.a.
• Molecular weight: 17619.91 Da
• Isoelectric Point: 4.5152

>T228268 WP_152135257.1 NZ_CP089442:6407-6877 [Klebsiella quasipneumoniae]
VILYRLTKTKYLSTAWTGYCAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPDNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Nucleotide

• Length: 471 bp

>T228268 NZ_CP123598:c4152959-4152856 [Enterobacter cloacae]
GGCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTATTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 148 a.a.
• Molecular weight: 16508.82 Da
• Isoelectric Point: 10.3013

>AT228268 WP_032413443.1 NZ_CP089442:5967-6410 [Klebsiella quasipneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVANRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDREAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Nucleotide

• Length: 444 bp

>AT228268 NZ_CP123598:4152843-4152994 [Enterobacter cloacae]
CTTTCAATCAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGC
TAAAAGTTGGCATTAATGCAGGCTAAGTCGCCTTGCCTTTTAAGAATAGATGACGACGCCAGGTTTTCCAGT

Structures

Antitoxin

• AlphaFold DB: A0A7H4PRG4