Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 4438265..4439035 Replicon chromosome
Accession NZ_CP089437
Organism Citrobacter portucalensis strain GD21SC92T

Toxin (Protein)


Gene name hipA Uniprot ID -
Locus tag LT980_RS21545 Protein ID WP_250671438.1
Coordinates 4438265..4438648 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID -
Locus tag LT980_RS21550 Protein ID WP_186919298.1
Coordinates 4438784..4439035 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LT980_RS21515 (LT980_21505) 4433537..4433827 + 291 WP_014908067.1 hypothetical protein -
LT980_RS21520 (LT980_21510) 4433892..4434797 + 906 WP_250671436.1 zincin-like metallopeptidase domain-containing protein -
LT980_RS21525 (LT980_21515) 4434989..4435360 + 372 WP_134769819.1 hypothetical protein -
LT980_RS21530 (LT980_21520) 4435520..4437109 + 1590 WP_250671437.1 MobH family relaxase -
LT980_RS21535 (LT980_21525) 4437165..4437818 - 654 WP_079775607.1 HNH endonuclease -
LT980_RS21540 (LT980_21530) 4437930..4438196 - 267 WP_186919296.1 hypothetical protein -
LT980_RS21545 (LT980_21535) 4438265..4438648 - 384 WP_250671438.1 HipA N-terminal domain-containing protein Toxin
LT980_RS21550 (LT980_21540) 4438784..4439035 - 252 WP_186919298.1 helix-turn-helix domain-containing protein Antitoxin
LT980_RS21555 (LT980_21545) 4439708..4440607 + 900 WP_015959329.1 SMEK domain-containing protein -
LT980_RS21560 (LT980_21550) 4440911..4441576 - 666 WP_015959328.1 class I SAM-dependent methyltransferase -
LT980_RS21565 (LT980_21555) 4441577..4442272 - 696 WP_250671439.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - - 4379064..4444595 65531


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(1-59)

Antitoxin

Cro/C1-type helix-turn-helix domain

(10-64)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14799.85 Da        Isoelectric Point: 5.6855

>T228246 WP_250671438.1 NZ_CP089437:c4438648-4438265 [Citrobacter portucalensis]
MPLREERYRGKIVYNFFDNLLPDSEQIRSRIQVRFHVPTKQPFDLLARIGMDCVGAIQLWPYETNIPSVQREDAEPLTEN
EIEHLLQRYKDAPLGMQAEVDDFRISLAGAQEKTALQRVFRSHLVSS

Download         Length: 384 bp

>T228246 NZ_CP123578:2502568-2502918 [Priestia megaterium]
TTGGTAGTCAAACGTGGCGACGTTTATTTTGCTGACCTATCTCCTGTTGTTGGCTCAGAACAAGGAGGCGTTCGCCCTGT
ACTTGTCATTCAAAATGATATAGGCAATCGTTTTAGCCCAACTGTTATTGTAGCTGCGATCACTGCTCAAATTCAAAAGG
CAAAGTTACCTACGCATGTTGAAATTGATGCAAAGCGCTATGGATTTGAGCGAGATTCAGTTATTTTACTAGAACAAATT
CGTACTATTGATAAACAGAGGCTAACAGACAAGATTACCCATTTAGATGATGAAATGATGGACAGAGTGGATGAAGCCTT
GCAAATCAGTGTAGGACTTATCGACTTTTAG

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9520.88 Da        Isoelectric Point: 10.2470

>AT228246 WP_186919298.1 NZ_CP089437:c4439035-4438784 [Citrobacter portucalensis]
MNVTSPKMLAQAVREARKQRKLTQREAAESMSMKQSTVSEFENHPEGTRLDTLFKLLAALDLELQVVSRKGKKKSSEGWD
QEW

Download         Length: 252 bp

>AT228246 NZ_CP123578:2502282-2502563 [Priestia megaterium]
GTGTCTGAAGCGAGCGCAACTACAGAAATCCTAGTAAGATTACCGAAAAATTTAGTATCGGAGCTCGATCTTATGGTTAA
ACAAGAAAATGGCAACCGAAGTGATTTTATTCACCAAGCAACAAAATTGTACCTTCGTGAGCGAAAAAAACGCCATATTC
GCGAATCAATGAGACGTGGATATATGGAGATGGCAAAGATAAACTTAAATATTGCTTCCGAGGCTTTTCTAGCAGAGTAT
GAAGCTGATCATACTGTGGATCGTTTAGTAAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References