Detailed information of TA system TA228234

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yfjZ-ypjF/CbtA-CbeA
• Location: 882764..883425
• Replicon: chromosome
• Accession: NZ_CP089437
• Organism: Citrobacter portucalensis strain GD21SC92T

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: -
• Locus tag: LT980_RS04290
• Protein ID: WP_048997879.1
• Coordinates: 883102..883425 (+)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: A0A241Q747
• Locus tag: LT980_RS04285
• Protein ID: WP_048997880.1
• Coordinates: 882764..883081 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LT980_RS04250 (LT980_04250)	878727..879437	+	711	WP_250671744.1	DeoR family transcriptional regulator	-
LT980_RS04255 (LT980_04255)	879463..879999	+	537	WP_136345363.1	DUF4339 domain-containing protein	-
LT980_RS04260 (LT980_04260)	880236..881144	+	909	WP_250671745.1	Ivy family c-type lysozyme inhibitor	-
LT980_RS04265 (LT980_04265)	881243..881479	+	237	WP_136345380.1	DUF905 family protein	-
LT980_RS04270 (LT980_04270)	881565..882023	+	459	WP_250671746.1	antirestriction protein	-
LT980_RS04275 (LT980_04275)	882039..882515	+	477	WP_250671747.1	RadC family protein	-
LT980_RS04280 (LT980_04280)	882524..882745	+	222	WP_250671748.1	DUF987 domain-containing protein	-
LT980_RS04285 (LT980_04285)	882764..883081	+	318	WP_048997880.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
LT980_RS04290 (LT980_04290)	883102..883425	+	324	WP_048997879.1	TA system toxin CbtA family protein	Toxin
LT980_RS04295 (LT980_04295)	883668..884204	-	537	WP_060683281.1	GNAT family N-acetyltransferase	-
LT980_RS04300 (LT980_04300)	885021..887483	+	2463	WP_250671749.1	poly-beta-1,6 N-acetyl-D-glucosamine export porin PgaA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-107)

Antitoxin

• Antitoxin CbeA (5-104)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 12455.32 Da
• Isoelectric Point: 6.4624

>T228234 WP_048997879.1 NZ_CP089437:883102-883425 [Citrobacter portucalensis]
MKSQPATTQRAAKPCLSPVDVWQMLLTRLLEQHYGLTLNDTPFSEERVIQEHIDAGITLADAINFLVEKYELVRIDRRGF
SWQEQTPYLTIIDIMRARRDLGLLNRN

Nucleotide

• Length: 324 bp

>T228234 NZ_CP123549:20589-20870 [Arsenophonus nasoniae]
ATGAGCTATAGTATTGAATTTGAAAAACGCGCATTAAAAGAGTGGAAAAAATTAGCTCATCCAATACGTGAGCAATTTAA
GAAAAAGTTAGTTAAAGTTCTGGAATATCCACATATAAAATCTGCACAATTACACGGACATCAATATCGGTACAAAATCA
AACTAAAATCATCTGGATATCGATTAGTTTATGAAGTAAACGATAATAAACTTTTATTGCTCGTTATTGCCGTGGGAATA
AGAGCTAATAACGAAGTGTATCAGCTAGCAAGCAAACGTTAA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11993.64 Da
• Isoelectric Point: 7.6976

>AT228234 WP_048997880.1 NZ_CP089437:882764-883081 [Citrobacter portucalensis]
MSNITWGLQRDITPRLGARLVQEGNRLHYLADRASITGQFSDAECRKLDVAFPYFIRQMESMLTTGELNPRHTHCVTLYR
NGFTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 318 bp

>AT228234 NZ_CP123549:20348-20599 [Arsenophonus nasoniae]
ATGACTTATGAAATATTAACGAGTACGGTTGCAAGCATTACCGATTTAAAACGCAATCCAATGGGTACAGTCGCTGAAGC
AGAAGGAAATGCAGTTGCCATCCTTAACCGAAATGAACCAGCTTTTTACTGCGTACCACCTGATTTATATGCTTATTTTC
AAGAATTGGCAGAAGATACCGAGCTTAATCGTATTGCTGATGAACGCATGAAAAACCCTGAGTTTGTGAGTGTCAATATT
GATGAGCTATAG

Structures

Antitoxin

• AlphaFold DB: A0A241Q747