Detailed information of TA system TA228228

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 137237..138147
• Replicon: plasmid p1194_1
• Accession: NZ_CP089428
• Organism: Klebsiella pneumoniae strain 1194

Toxin (Protein)

• Gene name: -
• Uniprot ID: A0A2N4Z2R0
• Locus tag: A9X66_RS26565
• Protein ID: WP_004197882.1
• Coordinates: 137677..138147 (+)
• Length: 157 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A2J4RLY9
• Locus tag: A9X66_RS26560
• Protein ID: WP_004181895.1
• Coordinates: 137237..137680 (+)
• Length: 148 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A9X66_RS26535 (A9X66_026535)	133028..133450	+	423	WP_004197884.1	translesion error-prone DNA polymerase V autoproteolytic subunit	-
A9X66_RS26540 (A9X66_026540)	133450..133953	+	504	Protein_134	DNA polymerase V subunit UmuC	-
A9X66_RS26545 (A9X66_026545)	134030..134794	-	765	WP_004184534.1	IS21-like element helper ATPase IstB	-
A9X66_RS26550 (A9X66_026550)	134791..136290	-	1500	WP_004184532.1	IS21-like element ISPlge2 family transposase	-
A9X66_RS26555 (A9X66_026555)	136387..137136	+	750	Protein_137	DUF4113 domain-containing protein	-
A9X66_RS26560 (A9X66_026560)	137237..137680	+	444	WP_004181895.1	DUF2384 domain-containing protein	Antitoxin
A9X66_RS26565 (A9X66_026565)	137677..138147	+	471	WP_004197882.1	RES family NAD+ phosphorylase	Toxin
A9X66_RS26570 (A9X66_026570)	138257..138517	-	261	WP_004026352.1	hypothetical protein	-
A9X66_RS26575 (A9X66_026575)	139207..140222	+	1016	Protein_141	IS3-like element ISKpn8 family transposase	-
A9X66_RS26585 (A9X66_026585)	140991..141113	+	123	Protein_143	IS3 family transposase	-
A9X66_RS26590 (A9X66_026590)	141291..142631	+	1341	WP_004196353.1	IS1202-like element ISEsa1 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaTEM-1A / blaOXA-9 / ant(3'')-Ia / aac(6')-Ib / sul1 / qacE / aadA2 / dfrA15 / ARR-3 / dfrA8 / rmtG / sul2 / catA1 / formA	-	1..266289	266289
	-	inside	IScluster/Tn	-	-	129054..140615	11561

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-148)

Antitoxin

• Antitoxin Xre-like, helix-turn-helix domain (30-89)

Sequences

Toxin

Protein

• Length: 157 a.a.
• Molecular weight: 17633.92 Da
• Isoelectric Point: 4.5152

>T228228 WP_004197882.1 NZ_CP089428:137677-138147 [Klebsiella pneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDFFTLLSIDVPDELIQSANMD
ELPDNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Nucleotide

• Length: 471 bp

>T228228 NZ_CP123529:c12806-12525 [Arsenophonus nasoniae]
ATGAGCTATAGTATTGAATTTGAAAAACGCGCATTAAAAGAGTGGAAAAAATTAGCTCATCCAATACGTGAGCAATTTAA
GAAAAAGTTAGTTAAAGTTCTGGAATATCCACATATAAAATCTGCACAATTACACGGACATCAATATCGGTACAAAATCA
AACTAAAATCATCTGGATATCGATTAGTTTATGAAGTAAACGATAATAAACTTTTATTGCTCGTTATTGCCGTGGGAATA
AGAGCTAATAACGAAGTGTATCAGCTAGCAAGCAAACGTTAA

Antitoxin

Protein

• Length: 148 a.a.
• Molecular weight: 16536.87 Da
• Isoelectric Point: 10.3013

>AT228228 WP_004181895.1 NZ_CP089428:137237-137680 [Klebsiella pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVVNRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDREAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Nucleotide

• Length: 444 bp

>AT228228 NZ_CP123529:c13047-12796 [Arsenophonus nasoniae]
ATGACTTATGAAATATTAACGAGTACGGTTGCAAGCATTACCGATTTAAAACGCAATCCAATGGGTACAGTCGCTGAAGC
AGAAGGAAATGCAGTTGCCATCCTTAACCGAAATGAACCAGCTTTTTACTGCGTACCACCTGATTTATATGCTTATTTTC
AAGAATTGGCAGAAGATACCGAGCTTAATCGTATTGCTGATGAACGCATGAAAAACCCTGAGTTTGTGAGTGTCAATATT
GATGAGCTATAG

Structures

Toxin

• AlphaFold DB: A0A2N4Z2R0

Antitoxin

• AlphaFold DB: A0A2J4RLY9