Detailed information of TA system TA022822

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): zeta-pezA/zeta-couple_hipB
• Location: 1282531..1283765
• Replicon: chromosome
• Accession: NC_010582
• Organism: Streptococcus pneumoniae CGSP14

Toxin (Protein)

• Gene name: zeta
• Uniprot ID: B2IQC7
• Locus tag: SPCG_RS06695
• Protein ID: WP_000512672.1
• Coordinates: 1282531..1283289 (-)
• Length: 253 a.a.

Antitoxin (Protein)

• Gene name: pezA
• Uniprot ID: -
• Locus tag: SPCG_RS06700
• Protein ID: WP_000579607.1
• Coordinates: 1283289..1283765 (-)
• Length: 159 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SPCG_RS06675	1278171..1279094	+	924	WP_000417606.1	protein rep	-
SPCG_RS06680	1279890..1280609	-	720	Protein_1251	type A chloramphenicol O-acetyltransferase	-
SPCG_RS06685	1280780..1281028	-	249	WP_001814254.1	hypothetical protein	-
SPCG_RS06690	1281267..1282055	-	789	WP_000214003.1	hypothetical protein	-
SPCG_RS06695	1282531..1283289	-	759	WP_000512672.1	zeta toxin family protein	Toxin
SPCG_RS06700	1283289..1283765	-	477	WP_000579607.1	helix-turn-helix domain-containing protein	Antitoxin
SPCG_RS06705	1283835..1284107	-	273	WP_000495438.1	hypothetical protein	-
SPCG_RS06710	1284145..1284528	-	384	WP_000159212.1	hypothetical protein	-
SPCG_RS06715	1284525..1284758	-	234	WP_000152419.1	hypothetical protein	-
SPCG_RS06720	1284809..1285894	-	1086	WP_000401903.1	toprim domain-containing protein	-
SPCG_RS06725	1285904..1286545	-	642	WP_000933083.1	hypothetical protein	-
SPCG_RS06730	1286705..1286902	+	198	WP_001841477.1	hypothetical protein	-
SPCG_RS06735	1286916..1287212	-	297	WP_000998886.1	hypothetical protein	-
SPCG_RS06740	1287226..1287510	-	285	WP_000969638.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	cat(pC194) / erm(B) / aph(3')-III	-	1259712..1322879	63167

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (24-214)

Antitoxin

• Cro/C1-type helix-turn-helix domain (3-60)

Sequences

Toxin

Protein

• Length: 253 a.a.
• Molecular weight: 28861.84 Da
• Isoelectric Point: 8.6386

>T22822 WP_000512672.1 NC_010582:c1283289-1282531 [Streptococcus pneumoniae CGSP14]
MGIQDYTDSQFKQALARNIRSLTRGKKSSKQPIAILLGGQSGAGKTTIHRIKQKEFQGNIVIIDGDSFRSQHPHYLELQQ
EYGKDSVEYTKYFAGKMVESLVRELSHLGYNLLIEGTLRTIDVPKKTAQLLKSRGYEIQLALIATKPKLSYLSTLIRYEE
LYAINPNQARATPKEHHDFIVNHLVDNTRQLEELAIFERIQIYQRDRGCVYDAKENTTSAADVLQELLFGEWSQVEKDML
KSGEERLKDLTN

Nucleotide

• Length: 759 bp

>T22822 NC_010582:c1283289-1282531 [Streptococcus pneumoniae CGSP14]
ATGGGAATCCAAGATTATACCGATAGTCAATTTAAACAGGCTTTAGCAAGGAACATTCGCTCACTGACAAGAGGAAAAAA
GTCCAGTAAGCAACCTATAGCGATTTTGCTCGGAGGGCAAAGTGGTGCCGGTAAGACTACAATTCATCGTATTAAACAGA
AAGAATTTCAAGGAAATATTGTTATCATAGATGGAGATAGTTTTCGTTCTCAGCATCCACACTATTTAGAACTTCAGCAA
GAATATGGCAAAGACAGTGTTGAATACACCAAATATTTTGCAGGTAAAATGGTAGAGTCTTTAGTAAGAGAATTGAGTCA
TTTGGGATACAATCTTTTGATAGAGGGAACTTTACGAACTATTGATGTTCCAAAGAAAACAGCACAACTCTTGAAAAGTA
GGGGATATGAAATACAATTAGCCTTGATTGCGACAAAGCCTAAGCTGTCCTATCTGAGCACCCTTATCCGATACGAAGAA
CTGTACGCTATTAACCCAAATCAAGCACGCGCAACTCCAAAAGAACATCATGATTTCATTGTAAATCATCTAGTTGATAA
TACACGACAATTGGAAGAACTAGCTATCTTTGAAAGAATTCAAATTTACCAACGAGATAGAGGTTGTGTATATGATGCAA
AAGAAAATACAACTTCAGCGGCAGATGTTCTTCAAGAGTTACTGTTTGGGGAGTGGAGTCAAGTAGAGAAGGATATGCTT
AAATCTGGAGAAGAAAGATTGAAAGATTTAACTAATTGA

Antitoxin

Protein

• Length: 159 a.a.
• Molecular weight: 18279.74 Da
• Isoelectric Point: 4.4188

>AT22822 WP_000579607.1 NC_010582:c1283765-1283289 [Streptococcus pneumoniae CGSP14]
MIGKNIKSLRKTHDLTQLEFARIVGISRNSLSRYENGTSSVSTELIDIICQKFNVSYVDIVGEDKMLNPVEDYELTLKIE
IVKERGANLLSRLYRYQDSQGISIDDESNPWILMSDDLSDLIHTNIYLVETFDEIERYSGYLDGIERMLEIFEKRMAA

Nucleotide

• Length: 477 bp

>AT22822 NC_010582:c1283765-1283289 [Streptococcus pneumoniae CGSP14]
ATGATTGGAAAGAACATAAAATCCTTGCGTAAAACACATGACTTAACACAACTCGAATTTGCACGGATTGTAGGTATTTC
ACGAAATAGTCTGAGTCGTTATGAAAATGGAACGAGTTCAGTCTCTACCGAATTAATAGACATCATTTGTCAGAAGTTTA
ATGTATCTTATGTCGATATTGTAGGAGAAGATAAAATGCTCAATCCTGTTGAAGATTATGAATTGACTTTAAAAATTGAA
ATTGTGAAAGAAAGAGGTGCTAATCTATTATCTCGACTCTATCGTTATCAAGATAGTCAGGGAATTAGCATTGATGATGA
ATCTAATCCTTGGATTTTAATGAGTGATGATCTATCTGACTTGATTCATACGAATATCTATTTAGTAGAAACTTTTGATG
AAATAGAGAGATATAGTGGCTATTTGGATGGAATTGAACGTATGTTAGAGATATTTGAAAAACGGATGGCAGCCTAA

Structures

Toxin

• AlphaFold DB: A0A4J1Y0E8