Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 187913..188439 Replicon plasmid pKOX101_1
Accession NZ_CP089408
Organism Klebsiella michiganensis strain Kox101

Toxin (Protein)


Gene name relE Uniprot ID A0A7H4N9C7
Locus tag LUW94_RS30030 Protein ID WP_004118126.1
Coordinates 187913..188200 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag LUW94_RS30035 Protein ID WP_000534858.1
Coordinates 188200..188439 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LUW94_RS30010 (LUW94_30010) 183106..183954 - 849 WP_004118114.1 oxidoreductase -
LUW94_RS30015 (LUW94_30015) 184430..185575 + 1146 WP_223175646.1 MFS transporter -
LUW94_RS30020 (LUW94_30020) 185788..187053 + 1266 WP_154923646.1 hypothetical protein -
LUW94_RS30025 (LUW94_30025) 187680..187841 - 162 WP_004118124.1 type I toxin-antitoxin system Hok family toxin -
LUW94_RS30030 (LUW94_30030) 187913..188200 - 288 WP_004118126.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
LUW94_RS30035 (LUW94_30035) 188200..188439 - 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
LUW94_RS30040 (LUW94_30040) 188464..188560 + 97 Protein_200 hypothetical protein -
LUW94_RS30045 (LUW94_30045) 188569..189730 - 1162 Protein_201 IS30-like element ISCfr4 family transposase -
LUW94_RS30050 (LUW94_30050) 189985..190800 + 816 WP_004118130.1 SDR family oxidoreductase -
LUW94_RS30055 (LUW94_30055) 190830..191591 + 762 WP_004118132.1 SDR family NAD(P)-dependent oxidoreductase -
LUW94_RS30060 (LUW94_30060) 191631..192641 + 1011 WP_224244032.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - pla 1..220391 220391


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(6-83)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11223.16 Da        Isoelectric Point: 10.1967

>T228201 WP_004118126.1 NZ_CP089408:c188200-187913 [Klebsiella michiganensis]
MAYFLDFDERALKEWRKLGSTVREQLKKKLAEVLESPRIDANKLRGMHDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T228201 NZ_CP123498:c1802302-1800986 [Arsenophonus nasoniae]
ATGGTAATGGAAGTTATCAATGTCAACTATAAGCAACATGAAGTGGGTGCAGTAAGTTTTGATACTAGCAAAGGTTTAGG
TTTTTTTGAATACAATTCTCGCTTTATCAAACAAGGAATCGAGTTATCGCCATTGAAAATGCCATTAGCAACGCGAATTT
ATAGTTTTCCCGAATTGGATTTTAATACCTTTAAAGGATTACCTGGTTTAATTGCTGACTCGTTACCCGATGACTTTGGT
AATGCAGTACTTAATGCTTGGGTTGCGACGATGGGTAAATCTCCTGACACCATTACACCATTACAGCGACTACAATATAC
CGGTAAAAGAGGGATGGGCGCGTTGGAATACGTGCCAGCAACTAGGATCAAAAGTCTCAATGCCTCACAACAGGTTGAGA
TCAGTTCTCTGGTTGCTATTACTCAAGAAATTCTTGATTCAAGACAAAAATTTACCGTTGAGCTACATAAAAATGGTCAG
GAAGATCGCGAAGCAATGATGGCTCTGCTTTCAGTTGGCATGAGTGCTGGTGGCGCGAGACCAAAAGCCGTTCTAGCTTT
TAATCAAGATTTCACCCAGGTTCGTTCGGGACAAACTAATGTTCCGGACGGTTTTACCCATTATTTAATAAAGTTTGATG
GTGTTAGCGAACATAATAAAAATCAACAAACCTTTGGTGATCCAGTTGGTTTTGGTGCAATGGAATATGTTTATTATTTA
TTGGCAACCCGCTGTGGCATAGAAATGATGCCTTGTCGGCTGCTTGCTGAAGGTAATAGACGCCATTTTATTACTCAGCG
CTTTGATCGTTTTGGTAATAAAAAACGCCATGTACAAACCTTAAATGGCCTTGCTCACGTTGATTACAAAAAACCCGGCT
CTTTCTCCTACGCCGAATTATTCGGTGTTGCTAGACAATTAAAACTTTCAGCAAAAGAAGCAGAACAACTACTAAAAAGA
ATGACCTTTAATATTGTGGCACGCAATCATGATGATCACGCTAAAAATTTTTCTTTTATGCTAAAAGGCGATAAGTGGAT
GTTAGCCCCCGCCTATGATTTAGCTTATAGCTACAAGCCCGGTAGTAAATGGGTAAACAGTCATTGGATGAGCTTAAATG
GGAAACGTGACAATTTTTCTCGCCAAGATTTTTACTCATTAGAAAAGTTAAGTCCCCTGTTTACTCGAGAAATGATCAAC
GGAATTATTGATGAAACAATAGAAAAAGTTTCAGAGTGGCAAAAACTCGCTGTTGAGTTTGCAGTACCTAATTTGTTAGC
GCAGGAAATTAACGCAAATTTATGTTTAAATATCTAG

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT228201 WP_000534858.1 NZ_CP089408:c188439-188200 [Klebsiella michiganensis]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT228201 NZ_CP123498:c1802625-1802296 [Arsenophonus nasoniae]
ATGAGCATATTTGAAGAATCAGCTTCAGCGATCGCTGCGGAATTGGGCGAACGTTTGAAACAAGCTCGATTAAATCATGA
TTTAACGCAAGCTGAAGTAGCTGAACTGTCCGGTATCAGCCGAAAAACGGTCCTAAATGCAGAAAAAGGTAACGTCCAAT
TAGAGAACTTAATCGCTATTATGGCAGCATTGAATTTGCTTGAACAATTAGATCTATTTTTGCCTAAACAGGATATCTCA
CCGATTCAACTTGCTAAGTTACAAGGTAAAAAAAGACAACGCGCATCAGGGCATAGAAAAAATAATACAGAGGATTTAGC
AGAATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7H4N9C7

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References