Detailed information of TA system TA228200

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 119327..120063
• Replicon: plasmid pKOX101_1
• Accession: NZ_CP089408
• Organism: Klebsiella michiganensis strain Kox101

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: LUW94_RS29670
• Protein ID: WP_049083183.1
• Coordinates: 119581..120063 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: -
• Locus tag: LUW94_RS29665
• Protein ID: WP_049083184.1
• Coordinates: 119327..119593 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LUW94_RS29645 (LUW94_29645)	114684..115328	-	645	Protein_121	IS3 family transposase	-
LUW94_RS29650 (LUW94_29650)	115492..116001	+	510	WP_049082931.1	SMI1/KNR4 family protein	-
LUW94_RS29655 (LUW94_29655)	116024..117691	+	1668	WP_049082932.1	hypothetical protein	-
LUW94_RS29660 (LUW94_29660)	117705..118904	+	1200	WP_053086565.1	hypothetical protein	-
LUW94_RS29665 (LUW94_29665)	119327..119593	+	267	WP_049083184.1	DUF1778 domain-containing protein	Antitoxin
LUW94_RS29670 (LUW94_29670)	119581..120063	+	483	WP_049083183.1	GNAT family N-acetyltransferase	Toxin
LUW94_RS29675 (LUW94_29675)	120270..121613	+	1344	WP_077259692.1	ISNCY family transposase	-
LUW94_RS29680 (LUW94_29680)	121684..122502	-	819	WP_023307220.1	abortive infection family protein	-
LUW94_RS29685 (LUW94_29685)	122511..123083	-	573	WP_032676095.1	recombinase family protein	-
LUW94_RS29690 (LUW94_29690)	123371..123790	-	420	WP_224250481.1	hypothetical protein	-
LUW94_RS29695 (LUW94_29695)	124032..125000	-	969	WP_232391991.1	IS5 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	pla	1..220391	220391
	-	inside	IScluster/Tn	-	-	110040..125000	14960

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (2-87)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17326.90 Da
• Isoelectric Point: 9.1188

>T228200 WP_049083183.1 NZ_CP089408:119581-120063 [Klebsiella michiganensis]
VGRITAPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYTHHGFTASQTHERTLFLKLP

Nucleotide

• Length: 483 bp

>T228200 NZ_CP123498:c1527895-1527515 [Arsenophonus nasoniae]
ATGCAAACTTTACCGTTCCCCGAGCCACGGGCGGCTGAGCCATGCCCGTCGCCCGTGCAGGTCTGGCAGCTGCTACTCGC
ACATCTGCTGGATAAACACTATGGCCTCACGCTCAACGACACGCCGTTTGGGGACAACAGCGTGATTCAGGCGCACATCA
ACGCCGGTATCTCCCTGAGTGATGCTGTCAATTTCATTGTGGAAAGATATGAACTTGTTCGCATCGACCACAGCCGTTTC
AGCCTCACCGAACGCTCTTCACTGATTGGTGCCATCGACATTCTGCGTGCCCGTCGTGCAACTGGGCTGATAGTGAGCAG
CAGCTACAGCACCATAACCCGCATCACAACGGGCCGGTATCGGGAGGACAAAACACAGTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10117.61 Da
• Isoelectric Point: 4.6437

>AT228200 WP_049083184.1 NZ_CP089408:119327-119593 [Klebsiella michiganensis]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDVQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT228200 NZ_CP123498:c1528252-1527926 [Arsenophonus nasoniae]
ATGCATTCTCAACAATGGGGGCTGACGTGTAGTAAAACGCCGTGCTTCGGTGCCAGGCTGGTGCAGGAAGGCAACCGACT
GCACTTTCTGGCTGACCGGGCAGGATTTACCGGCGACTTCAGCGACGATGAAGCACTTCGCCTTGACCAGGCTTTTCCGC
TGATAATGAAACAGCTGGAGCAGATGATGTCAGCCGGTGAGCTGAATCCCCTCCAGCAGCAACGCGTTACCCTGCATCAC
AACGGGCTGACCTGCCAGGCCGATACCCTCGGTTCACACGGTTATGTTTACATCGCGATTTATTCCTCTTCGACTGCAAC
CGGCTGA