Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 119327..120063 Replicon plasmid pKOX101_1
Accession NZ_CP089408
Organism Klebsiella michiganensis strain Kox101

Toxin (Protein)


Gene name tacT Uniprot ID -
Locus tag LUW94_RS29670 Protein ID WP_049083183.1
Coordinates 119581..120063 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID -
Locus tag LUW94_RS29665 Protein ID WP_049083184.1
Coordinates 119327..119593 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LUW94_RS29645 (LUW94_29645) 114684..115328 - 645 Protein_121 IS3 family transposase -
LUW94_RS29650 (LUW94_29650) 115492..116001 + 510 WP_049082931.1 SMI1/KNR4 family protein -
LUW94_RS29655 (LUW94_29655) 116024..117691 + 1668 WP_049082932.1 hypothetical protein -
LUW94_RS29660 (LUW94_29660) 117705..118904 + 1200 WP_053086565.1 hypothetical protein -
LUW94_RS29665 (LUW94_29665) 119327..119593 + 267 WP_049083184.1 DUF1778 domain-containing protein Antitoxin
LUW94_RS29670 (LUW94_29670) 119581..120063 + 483 WP_049083183.1 GNAT family N-acetyltransferase Toxin
LUW94_RS29675 (LUW94_29675) 120270..121613 + 1344 WP_077259692.1 ISNCY family transposase -
LUW94_RS29680 (LUW94_29680) 121684..122502 - 819 WP_023307220.1 abortive infection family protein -
LUW94_RS29685 (LUW94_29685) 122511..123083 - 573 WP_032676095.1 recombinase family protein -
LUW94_RS29690 (LUW94_29690) 123371..123790 - 420 WP_224250481.1 hypothetical protein -
LUW94_RS29695 (LUW94_29695) 124032..125000 - 969 WP_232391991.1 IS5 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - pla 1..220391 220391
- inside IScluster/Tn - - 110040..125000 14960


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(2-87)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17326.90 Da        Isoelectric Point: 9.1188

>T228200 WP_049083183.1 NZ_CP089408:119581-120063 [Klebsiella michiganensis]
VGRITAPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYTHHGFTASQTHERTLFLKLP

Download         Length: 483 bp

>T228200 NZ_CP123498:c1527895-1527515 [Arsenophonus nasoniae]
ATGCAAACTTTACCGTTCCCCGAGCCACGGGCGGCTGAGCCATGCCCGTCGCCCGTGCAGGTCTGGCAGCTGCTACTCGC
ACATCTGCTGGATAAACACTATGGCCTCACGCTCAACGACACGCCGTTTGGGGACAACAGCGTGATTCAGGCGCACATCA
ACGCCGGTATCTCCCTGAGTGATGCTGTCAATTTCATTGTGGAAAGATATGAACTTGTTCGCATCGACCACAGCCGTTTC
AGCCTCACCGAACGCTCTTCACTGATTGGTGCCATCGACATTCTGCGTGCCCGTCGTGCAACTGGGCTGATAGTGAGCAG
CAGCTACAGCACCATAACCCGCATCACAACGGGCCGGTATCGGGAGGACAAAACACAGTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10117.61 Da        Isoelectric Point: 4.6437

>AT228200 WP_049083184.1 NZ_CP089408:119327-119593 [Klebsiella michiganensis]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDVQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT228200 NZ_CP123498:c1528252-1527926 [Arsenophonus nasoniae]
ATGCATTCTCAACAATGGGGGCTGACGTGTAGTAAAACGCCGTGCTTCGGTGCCAGGCTGGTGCAGGAAGGCAACCGACT
GCACTTTCTGGCTGACCGGGCAGGATTTACCGGCGACTTCAGCGACGATGAAGCACTTCGCCTTGACCAGGCTTTTCCGC
TGATAATGAAACAGCTGGAGCAGATGATGTCAGCCGGTGAGCTGAATCCCCTCCAGCAGCAACGCGTTACCCTGCATCAC
AACGGGCTGACCTGCCAGGCCGATACCCTCGGTTCACACGGTTATGTTTACATCGCGATTTATTCCTCTTCGACTGCAAC
CGGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References