Detailed information of TA system TA022820

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pezAT/zeta-couple_hipB
• Location: 1000606..1001843
• Replicon: chromosome
• Accession: NC_010582
• Organism: Streptococcus pneumoniae CGSP14

Toxin (Protein)

• Gene name: pezT
• Uniprot ID: C1CE39
• Locus tag: SPCG_RS05345
• Protein ID: WP_000405356.1
• Coordinates: 1001082..1001843 (+)
• Length: 254 a.a.

Antitoxin (Protein)

• Gene name: pezA
• Uniprot ID: -
• Locus tag: SPCG_RS05340
• Protein ID: WP_000579605.1
• Coordinates: 1000606..1001082 (+)
• Length: 159 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SPCG_RS05315	995679..996533	+	855	WP_000900020.1	ADP-ribosylglycohydrolase family protein	-
SPCG_RS05320	996645..997529	+	885	WP_000710115.1	diacylglycerol kinase family lipid kinase	-
SPCG_RS05325	997692..999431	+	1740	WP_000423450.1	glycoside hydrolase family 13 protein	-
SPCG_RS05330	999517..999834	+	318	WP_000995820.1	hypothetical protein	-
SPCG_RS05335	999880..1000161	+	282	WP_000266233.1	hypothetical protein	-
SPCG_RS05340	1000606..1001082	+	477	WP_000579605.1	helix-turn-helix domain-containing protein	Antitoxin
SPCG_RS05345	1001082..1001843	+	762	WP_000405356.1	zeta toxin family protein	Toxin
SPCG_RS05350	1001871..1003027	+	1157	Protein_999	hypothetical protein	-
SPCG_RS05360	1003859..1004218	+	360	WP_000529543.1	hypothetical protein	-
SPCG_RS05365	1004228..1004593	+	366	WP_000263038.1	MobC family plasmid mobilization relaxosome protein	-
SPCG_RS05370	1004580..1006430	+	1851	WP_000261804.1	relaxase/mobilization nuclease domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	982140..1008118	25978

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (25-215)

Antitoxin

• Cro/C1-type helix-turn-helix domain (3-60)

Sequences

Toxin

Protein

• Length: 254 a.a.
• Molecular weight: 29078.11 Da
• Isoelectric Point: 7.1307

>T22820 WP_000405356.1 NC_010582:1001082-1001843 [Streptococcus pneumoniae CGSP14]
MEIQDYTDSEFKHALARNLRSLTRGKKSSKQPIAILLGGQSGAGKTTIHRIKQKEFQGNIVIIDGDSFRSQHPHYLELQQ
EYGKDSVEYTKDFAGKMVESLVTKLSSLGYNLLIEGTLRTIDVPKKTAQLLKNKGYEVQLALIATKPELSYLSTLIRYEE
LYIINPNQARATPKEHHDFIVNHLVDNTRKLEELAIFERIQIYQRDRSCVYDSKENTTSAADVLQELFFGEWSQVEKEML
QVGEKRLNELLEK

Nucleotide

• Length: 762 bp

>T22820 NC_010582:1001082-1001843 [Streptococcus pneumoniae CGSP14]
ATGGAAATCCAAGATTATACTGATAGTGAATTCAAACATGCTTTAGCAAGGAATCTTCGTTCACTGACAAGAGGAAAAAA
GTCCAGTAAGCAACCTATAGCGATTTTGCTTGGAGGTCAAAGTGGTGCCGGTAAGACTACAATTCATCGTATTAAACAGA
AAGAATTTCAAGGAAATATTGTTATCATAGATGGTGATAGTTTTCGTTCTCAGCATCCACACTATTTAGAACTGCAGCAA
GAATATGGCAAAGACAGTGTAGAATATACCAAAGATTTTGCAGGAAAAATGGTAGAGTCTTTAGTAACAAAATTGAGTAG
TTTGGGATACAATCTTTTGATCGAGGGAACTTTACGAACAATTGATGTTCCAAAGAAAACGGCACAACTCTTGAAAAATA
AGGGATATGAAGTACAATTGGCCTTAATTGCGACAAAGCCTGAATTGTCGTATCTAAGTACTCTTATCCGTTATGAAGAA
CTGTACATTATCAATCCAAATCAAGCACGCGCAACTCCAAAAGAACATCATGATTTCATTGTAAATCATCTAGTTGATAA
CACACGAAAATTGGAAGAACTAGCTATCTTTGAAAGAATTCAAATTTACCAACGAGATAGAAGTTGTGTATATGATTCAA
AAGAAAATACAACTTCAGCAGCAGATGTTCTTCAAGAGTTATTCTTTGGGGAGTGGAGTCAGGTAGAGAAGGAGATGTTG
CAGGTGGGGGAAAAGAGACTTAATGAATTACTTGAAAAATAA

Antitoxin

Protein

• Length: 159 a.a.
• Molecular weight: 18270.70 Da
• Isoelectric Point: 4.5673

>AT22820 WP_000579605.1 NC_010582:1000606-1001082 [Streptococcus pneumoniae CGSP14]
MIGKNIKSLRKTHDLTQHEFARIVGISRNSLSRYENGTSSVSTELIDIICQKFNVSYVDIVGENKMLNPVEDYELTLKIE
IVKERGANLLSRLYRYQDSQGISIDDESNPWILMSDDLSDLIHTNIYLVETFDEIERYSGYLDGIERMLEISEKRMVA

Nucleotide

• Length: 477 bp

>AT22820 NC_010582:1000606-1001082 [Streptococcus pneumoniae CGSP14]
ATGATTGGAAAGAACATAAAATCCTTACGTAAAACACATGACTTAACACAACACGAATTTGCACGGATTGTAGGTATTTC
ACGAAATAGTCTGAGTCGTTATGAAAATGGAACGAGTTCAGTCTCTACCGAATTAATAGACATCATTTGTCAGAAGTTTA
ATGTATCTTATGTCGATATCGTAGGAGAAAATAAAATGCTCAATCCTGTTGAAGATTATGAATTGACTTTAAAAATTGAA
ATTGTGAAAGAAAGAGGTGCTAATCTATTATCTCGACTCTATCGCTATCAAGATAGTCAGGGAATTAGCATTGATGATGA
ATCTAATCCTTGGATTTTAATGAGTGATGATCTTTCTGATTTGATTCATACGAATATCTATTTAGTAGAAACTTTTGATG
AAATAGAGAGATATAGTGGCTATTTGGATGGAATTGAACGTATGTTAGAGATATCTGAAAAGCGGATGGTAGCTTAA

Structures

Toxin

• AlphaFold DB: A0A4J1TI62