Detailed information of TA system TA228199

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 113942..114467
• Replicon: plasmid pKOX101_1
• Accession: NZ_CP089408
• Organism: Klebsiella michiganensis strain Kox101

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: J5VBT8
• Locus tag: LUW94_RS29635
• Protein ID: WP_004197633.1
• Coordinates: 113942..114247 (-)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: A0A9E1GDP8
• Locus tag: LUW94_RS29640
• Protein ID: WP_004197642.1
• Coordinates: 114249..114467 (-)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LUW94_RS29600 (LUW94_29600)	108967..109215	-	249	WP_004187025.1	plasmid stabilization protein	-
LUW94_RS29605 (LUW94_29605)	109593..109991	-	399	WP_049084174.1	helix-turn-helix domain-containing protein	-
LUW94_RS29610 (LUW94_29610)	110040..111386	-	1347	WP_077259693.1	ISNCY family transposase	-
LUW94_RS29615 (LUW94_29615)	111515..112285	+	771	WP_182205113.1	hypothetical protein	-
LUW94_RS29625 (LUW94_29625)	113031..113345	-	315	WP_074181342.1	hypothetical protein	-
LUW94_RS29630 (LUW94_29630)	113372..113773	-	402	WP_177343058.1	hypothetical protein	-
LUW94_RS29635 (LUW94_29635)	113942..114247	-	306	WP_004197633.1	type II toxin-antitoxin system toxin CcdB	Toxin
LUW94_RS29640 (LUW94_29640)	114249..114467	-	219	WP_004197642.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
LUW94_RS29645 (LUW94_29645)	114684..115328	-	645	Protein_121	IS3 family transposase	-
LUW94_RS29650 (LUW94_29650)	115492..116001	+	510	WP_049082931.1	SMI1/KNR4 family protein	-
LUW94_RS29655 (LUW94_29655)	116024..117691	+	1668	WP_049082932.1	hypothetical protein	-
LUW94_RS29660 (LUW94_29660)	117705..118904	+	1200	WP_053086565.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	pla	1..220391	220391
	-	inside	IScluster/Tn	-	-	110040..125000	14960

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11585.27 Da
• Isoelectric Point: 6.4661

>T228199 WP_004197633.1 NZ_CP089408:c114247-113942 [Klebsiella michiganensis]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDDSYRLMTTDMASVTASVTGEEV
ADLSHRENDIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T228199 NZ_CP123498:1458848-1459135 [Arsenophonus nasoniae]
ATGATGATTAAAAAATTAACAACTTATGATCCGGCTGAGGCGTTGGTCAATGATGAAGAAATTGCCTTTTTTATGGCCGA
TGCGCTGGAAACAGGCGATGCAAGTTATATCGCTAAAGCTATCGGTGTAGTGGCTCGGGCAAAGGGCATGACACAAATAG
CGGCACAAACCGGGCTATCCCGTGAACAGCTCTATCGCTCATTTAGTGACGAAGGTAACCCAACCTTAAAAACCACTTTG
GCAGTAATGAAAGCATTAGGCGTTGATTTAACCGTTAAACGCCATTAA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8376.34 Da
• Isoelectric Point: 4.7405

>AT228199 WP_004197642.1 NZ_CP089408:c114467-114249 [Klebsiella michiganensis]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT228199 NZ_CP123498:1458541-1458858 [Arsenophonus nasoniae]
ATGAAAGAACGTAAACTAAAACAAACCGCCGTTTTTCATGCCTGGGAATCCAAGTTAAGGGATCGTCGGGCGAGAACCTT
GATTGCCACGCGTTTGTTTCGCCTGGCGAATGGATTAGCGGGCGATGTTATGCCGGTCGGGGAAGGGATCAGCGAACTGC
GTATTCATTATGGCCCGGGCTATCGGATTTATTTTCAACAGCGTGGTGACACGATTATTGTTTTGTTATGCGGTGGTGAT
AAAAGCACACAGTCCAACGATATTTTATTGGCTAAGAAATTAGCGAAAGAGTGGATTGATGAGGTGAATGATGATTAA

Structures

Toxin

• AlphaFold DB: A0A2A5MCG5