Detailed information of TA system TA228197

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 98413..99323
• Replicon: plasmid pKOX101_1
• Accession: NZ_CP089408
• Organism: Klebsiella michiganensis strain Kox101

Toxin (Protein)

• Gene name: -
• Uniprot ID: A0A2A5MBW7
• Locus tag: LUW94_RS29540
• Protein ID: WP_023329005.1
• Coordinates: 98853..99323 (+)
• Length: 157 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A9J6S5L1
• Locus tag: LUW94_RS29535
• Protein ID: WP_049084156.1
• Coordinates: 98413..98856 (+)
• Length: 148 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LUW94_RS29510 (LUW94_29510)	94305..94535	-	231	Protein_94	hypothetical protein	-
LUW94_RS29515 (LUW94_29515)	94734..95455	+	722	Protein_95	DUF969 domain-containing protein	-
LUW94_RS29520 (LUW94_29520)	95452..96438	+	987	Protein_96	DUF979 domain-containing protein	-
LUW94_RS29525 (LUW94_29525)	96448..97443	+	996	WP_049084154.1	DUF2891 domain-containing protein	-
LUW94_RS29530 (LUW94_29530)	97625..98291	+	667	Protein_98	DUF4113 domain-containing protein	-
LUW94_RS29535 (LUW94_29535)	98413..98856	+	444	WP_049084156.1	DUF2384 domain-containing protein	Antitoxin
LUW94_RS29540 (LUW94_29540)	98853..99323	+	471	WP_023329005.1	RES family NAD+ phosphorylase	Toxin
LUW94_RS29545 (LUW94_29545)	99505..99693	-	189	WP_023329006.1	hypothetical protein	-
LUW94_RS29550 (LUW94_29550)	100388..101763	+	1376	WP_094955560.1	IS3 family transposase	-
LUW94_RS29555 (LUW94_29555)	102012..102587	-	576	WP_032731970.1	cytochrome b/b6 domain-containing protein	-
LUW94_RS29560 (LUW94_29560)	102735..103040	+	306	WP_023328906.1	metalloregulator ArsR/SmtB family transcription factor	-
LUW94_RS29565 (LUW94_29565)	103042..103575	+	534	WP_035897527.1	rhodanese-like domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	pla	1..220391	220391
	-	flank	IS/Tn	-	-	101005..101763	758

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-148)

Antitoxin

• Antitoxin Xre (16-147)

Sequences

Toxin

Protein

• Length: 157 a.a.
• Molecular weight: 17459.70 Da
• Isoelectric Point: 4.4829

>T228197 WP_023329005.1 NZ_CP089408:98853-99323 [Klebsiella michiganensis]
VILYRLTKTKNLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPANWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHSDFYGIVQMAQQIPFRFDSRLKPDRK

Nucleotide

• Length: 471 bp

>T228197 NZ_CP123498:c1302298-1301969 [Arsenophonus nasoniae]
ATGAAATATACGATTGAGTATTACAGTGAAGAAGTTAGAACCGAAATCGAACAACTGCCTACCAGTATGCGAGTTAGATA
TACCCACCTGGTAGAACGCATGAAGGTATACGGAGGAAATCTCGGAGAACCTCATACTAGTCCTTTCGGGGATGGACTTT
TTGAGCTTCGTATCAAAAGCAGTAATGGTATTGGTCGTGTTTTCTACTGCACACTGATAGGAAAGTGCATTGTAATACTT
CACAGTTTTGTGAAGAAAACTCAGAAAACACCGGTGGGTGATCTCAAAAAAGCAAAAACCAGAATGAAAGAGGTAAAACA
TGACTGGTAA

Antitoxin

Protein

• Length: 148 a.a.
• Molecular weight: 16447.80 Da
• Isoelectric Point: 10.2498

>AT228197 WP_049084156.1 NZ_CP089408:98413-98856 [Klebsiella michiganensis]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVANRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDKEAALKWLNEPNRALSWKVPADLIASETGAYEVIKLITRLEHGVYS

Nucleotide

• Length: 444 bp

>AT228197 NZ_CP123498:c1301979-1301671 [Arsenophonus nasoniae]
ATGACTGGTAAACGTATTTCCCCAAAAATGACCCATGATGAAATGGTCGCTAAATGGATGAAAGATCCAGCCTTTAAAGC
TGAGTATGATGCTATTGAAGAAGAGTTTGCTCTGTTAGACGAAATTCTATTAGCCCGAAAAAAAGCAGGATTAACCCAGG
CGCAGCTTGCTGAGCGGATGGGCACTAAAGCCACCGCAATAACCCGTATGGAAAGTAACCTTGCTTCTGGTGTTAGTGGA
CCTTCATTTTCTACCCTAAAAAAATTAGCAAAAGCAACCGGCAAAAAATTACAAATCCGCTTTGTATAA

Structures

Toxin

• AlphaFold DB: A0A2A5MBW7