Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 4802264..4802811 Replicon chromosome
Accession NZ_CP089407
Organism Klebsiella michiganensis strain Kox101

Toxin (Protein)


Gene name ccdB Uniprot ID A0A0H3H5T3
Locus tag LUW94_RS22735 Protein ID WP_014227299.1
Coordinates 4802264..4802572 (-) Length 103 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID A0A0H3H0P7
Locus tag LUW94_RS22740 Protein ID WP_014227298.1
Coordinates 4802575..4802811 (-) Length 79 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LUW94_RS22710 (LUW94_22710) 4798168..4798479 - 312 WP_014227304.1 PTS sugar transporter subunit IIB -
LUW94_RS22715 (LUW94_22715) 4798774..4799715 + 942 WP_014227303.1 LacI family DNA-binding transcriptional regulator -
LUW94_RS22720 (LUW94_22720) 4799739..4800032 - 294 WP_202019229.1 YicS family protein -
LUW94_RS22725 (LUW94_22725) 4800242..4801207 + 966 WP_064375675.1 Rpn family recombination-promoting nuclease/putative transposase -
LUW94_RS22730 (LUW94_22730) 4801211..4802113 - 903 WP_009654083.1 DMT family transporter -
LUW94_RS22735 (LUW94_22735) 4802264..4802572 - 309 WP_014227299.1 CcdB family protein Toxin
LUW94_RS22740 (LUW94_22740) 4802575..4802811 - 237 WP_014227298.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
LUW94_RS22745 (LUW94_22745) 4802917..4804350 - 1434 WP_048262922.1 PTS N-acetylmuramic acid transporter subunit IIBC -
LUW94_RS22750 (LUW94_22750) 4804375..4805277 - 903 WP_032752002.1 N-acetylmuramic acid 6-phosphate etherase -
LUW94_RS22755 (LUW94_22755) 4805438..4806604 - 1167 WP_064406020.1 multidrug effflux MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-101)

Antitoxin

Post-segregation antitoxin CcdA

(8-78)


Sequences

Toxin        


Download         Length: 103 a.a.        Molecular weight: 11725.57 Da        Isoelectric Point: 7.2729

>T228194 WP_014227299.1 NZ_CP089407:c4802572-4802264 [Klebsiella michiganensis]
MQYYVYKNTGRIAAYPYLLDVQSDIIGKRNTRVVIPLFPLKNYKGPRADRLTPLVTVEGEEYVVMTHELASIPHRVLGEE
VCNLNHQREVVKASMDFLFDGI

Download         Length: 309 bp

>T228194 NZ_CP123498:556339-556668 [Arsenophonus nasoniae]
ATGAAATATACGATTGAGTATTACAGCGAAGAAGTTGAAAAAGACATCCTCTCCCTCCCTGACACCATTCAGGCAAGATA
CATTCGTTATACTGAAAAAATGCGTATTTATGGTGCTAACCTGGGAGAACCTCACACGAGTTCTTTTGGCGATGGCCTTT
TCGAACTCAGGCTTAAAGGCTGTGAAGGAATAGGTAGAGTCTTTTATTGCACATTGATAGGAAAACGGATTGTTATGTTG
CATAGTTTCATAAAAAAGACCAACAAGACACCTATCAAAGAACGCCAAAAAGCAGAAAAAAGACTGAAAGAGGTTAAATA
TGGCAAATAA

Antitoxin


Download         Length: 79 a.a.        Molecular weight: 8783.92 Da        Isoelectric Point: 5.7928

>AT228194 WP_014227298.1 NZ_CP089407:c4802811-4802575 [Klebsiella michiganensis]
MPAAKRNKQNVSVTLDPGLVKQAREVGINLSLTLTNALKSEIHSLEIARWQKENAQAIDYLNNLTEENGLLSDEYRTF

Download         Length: 237 bp

>AT228194 NZ_CP123498:556658-556960 [Arsenophonus nasoniae]
ATGGCAAATAAACGCACCATGATGACACATGATGAATTAGTCGAAAAAATGTTATCTAATCCAAAGGTAAAAGCAGAATA
CGATGCTATCGCTGAAGAATTTGCTCTGCTAGATGAGATTTTAGCTGCTCGCAAAAATGCAGGGTTAACACAAGAACAAC
TTGCTAAACGAATGGGCACTAAAGCGACCGCAATAACACGTATGGAAAGTAACCTTGCTTCTGGTGTTAGTGGACCTTCG
TTTTCTACCCTAAAAAAATTAGCAAAAGCAACCGGCAAAAAATTACAAATCCGCTTCGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3H5T3

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3H0P7

References