228191

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3337115..3337734 Replicon chromosome
Accession NZ_CP089407
Organism Klebsiella michiganensis strain Kox101

Toxin (Protein)


Gene name Hha Uniprot ID H3N9D8
Locus tag LUW94_RS15940 Protein ID WP_004099646.1
Coordinates 3337516..3337734 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID A0A0J2I7Z1
Locus tag LUW94_RS15935 Protein ID WP_025107145.1
Coordinates 3337115..3337489 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LUW94_RS15925 (LUW94_15925) 3332271..3333464 + 1194 WP_004136054.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
LUW94_RS15930 (LUW94_15930) 3333487..3336633 + 3147 WP_014228207.1 multidrug efflux RND transporter permease subunit AcrB -
LUW94_RS15935 (LUW94_15935) 3337115..3337489 + 375 WP_025107145.1 Hha toxicity modulator TomB Antitoxin
LUW94_RS15940 (LUW94_15940) 3337516..3337734 + 219 WP_004099646.1 HHA domain-containing protein Toxin
LUW94_RS15945 (LUW94_15945) 3337897..3338463 + 567 WP_032748289.1 maltose O-acetyltransferase -
LUW94_RS15950 (LUW94_15950) 3338435..3338569 - 135 WP_223226764.1 hypothetical protein -
LUW94_RS15955 (LUW94_15955) 3338590..3339060 + 471 WP_064357998.1 YlaC family protein -
LUW94_RS15960 (LUW94_15960) 3339035..3340489 - 1455 WP_064358105.1 PLP-dependent aminotransferase family protein -
LUW94_RS15965 (LUW94_15965) 3340591..3341289 + 699 WP_064374550.1 GNAT family protein -
LUW94_RS15970 (LUW94_15970) 3341286..3341426 - 141 WP_003859006.1 type B 50S ribosomal protein L36 -
LUW94_RS15975 (LUW94_15975) 3341426..3341689 - 264 WP_004848323.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-115)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8640.05 Da        Isoelectric Point: 8.9008

>T228191 WP_004099646.1 NZ_CP089407:3337516-3337734 [Klebsiella michiganensis]
MSDKTLTKIDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPPSVWKFIR

Download         Length: 219 bp

>T228191 NZ_CP123492:51510-51686 [Arsenophonus nasoniae]
GTGAAACAAAGCGAGTTCAGGCGGTGGCTTGAGGCGCAGGGGGTTGAAATTTCAAACGGGACAAATCATTTGAAATTGAG
GTTTAACGGCAAGCGAAGCGTGATGCCCCGACACCCCAGCGCAGAAATCAAAGAGCCAACGCGAAAAGCCATACTTAAAC
AGTTAGGCTTAAAATAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14366.15 Da        Isoelectric Point: 4.8989

>AT228191 WP_025107145.1 NZ_CP089407:3337115-3337489 [Klebsiella michiganensis]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIAAFALNYKIKYAEDNKLITQIDEYL
DDTFVLFSNYGINSADLQKWRKSGNRLFRCFVNACRENPASLSC

Download         Length: 375 bp

>AT228191 NZ_CP123492:51736-52146 [Arsenophonus nasoniae]
ATGAGATATCCGGTAAATTTAACAGCGTGTAAAGAGGGCGGGTTTTCGGTGACTTTCCCTGATATACCCGAAGCTATCAC
ACAAGGGGAAAACCGCGAAGAAGCCCTTAAAATGGCTCTGGATGCGCTCGTCACCGCGTTTGAGTTCTACTTTGAGGATA
ACGAGGCTATCCCCGCCCCCAGTCCTGTGAGAGCGGATTTTGTGGAAGTTCCAGCGGCAGTGGTTGCCAAAGTGCTACTA
CTCAATCAGTTTGCGAAGTCTGAAATGTCTCAAAGTGAATTAGCAAGGCGTATGCACTGCCCACGGCAGGAAGTGACACG
GTTGTTTGACCTGAAACACAACACAAAAATCAACACTGTTCAAAAAGCACTGGCAGTATTAGGGCAGCGACTTGAGCTAT
CCGTTGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3H713

Antitoxin

Source ID Structure
AlphaFold DB A0A0J2I7Z1

References