Detailed information of TA system TA228182

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 643596..644186
• Replicon: chromosome
• Accession: NZ_CP089407
• Organism: Klebsiella michiganensis strain Kox101

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: LUW94_RS02915
• Protein ID: WP_148841996.1
• Coordinates: 643854..644186 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: J6I3K7
• Locus tag: LUW94_RS02910
• Protein ID: WP_004852307.1
• Coordinates: 643596..643853 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LUW94_RS02895 (LUW94_02895)	640912..642117	-	1206	WP_064375861.1	hypothetical protein	-
LUW94_RS02900 (LUW94_02900)	642593..643054	+	462	WP_064375862.1	hypothetical protein	-
LUW94_RS02905 (LUW94_02905)	643051..643244	+	194	Protein_567	helix-turn-helix domain-containing protein	-
LUW94_RS02910 (LUW94_02910)	643596..643853	+	258	WP_004852307.1	antitoxin	Antitoxin
LUW94_RS02915 (LUW94_02915)	643854..644186	+	333	WP_148841996.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
LUW94_RS02925 (LUW94_02925)	644509..645966	+	1458	WP_025106072.1	EmmdR/YeeO family multidrug/toxin efflux MATE transporter	-
LUW94_RS02935 (LUW94_02935)	646930..648384	-	1455	WP_014229909.1	AMP nucleosidase	-
LUW94_RS02940 (LUW94_02940)	648517..648774	-	258	WP_014229908.1	histidine kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	627150..644186	17036

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-109)

Antitoxin

• Antitoxin PemI-like (4-84)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11878.79 Da
• Isoelectric Point: 10.8850

>T228182 WP_148841996.1 NZ_CP089407:643854-644186 [Klebsiella michiganensis]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPITSGGNFARTAGFTVSLEGAGTKTTGVIRCDQPRTI
DMAARNGKRLERIPDAVVNEVLARLDAILR

Nucleotide

• Length: 333 bp

>T228182 NZ_CP123431:c132290-131979 [Klebsiella pneumoniae]
ATGCACGTAATTTCAAAAGAGCCTTTTGAGGAAGCGGCAAAGCGATATCCCAACGATTCGTTAGCCATTCGGGCGTTGTA
CCGCTTAGTGCGCGAGACGGACTTCTCTTCTCCAGCTGAGATGCTAACTCTGATACCGAGCCTGGATAATTTTAAGTACA
GGAATAAGTGGTGGGTTTTGGATGTAGGGGGCAACAACTTGAGGGTTATCGCCTATATCAATTTCGTAAATAAGCGCTTC
TACGTGAAGCACATCGCAACCCACGCTGATTACGACAAGTTGACCCGCTATTACAGGGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9422.88 Da
• Isoelectric Point: 4.4607

>AT228182 WP_004852307.1 NZ_CP089407:643596-643853 [Klebsiella michiganensis]
MYTTRLKKVGGSIMLAVPPAVLKTLELSTDSEVGMTINNGCLIIEPQKRPHYSLEELLAQCDPHAEMSEEDREWIDAPAV
GKEIL

Nucleotide

• Length: 258 bp

>AT228182 NZ_CP123431:c131982-131563 [Klebsiella pneumoniae]
ATGATTACCGACACTGCAAAAGCCATTGAAGCAACCAAACAACTCGTAGCTGCCGTCCCCTTCCTAGGGGGCAGTTCTTC
CGAGAGCGATTACCGCGAAGCGATGGAGCTGGTGGATTATCTGATCGAGAACGACGACGAGAACCCACTTATTGACTTCC
TGGCGAGTAAGATTGCCGACTATGAAGATAACAGCCCTCGTTTCGCTGAGTTCAACAAAGCTATTGCTGAAATACCAGTC
GGCGTTGCACTTCTGCGAACCCTGATTGACCAGCACAAGCTGTCTTATTCAGACCTTAAGGATGAGATCGGATCTAAATC
ATTGGTCAGCCAAATCCTGTCAGGGCAGCGCTCTTTGACCATCACTCATATAAAAGCTCTTTCCGCGCGCTTCGGTGTAA
AACCTGAGTGGTTCCTATAA

Structures

Antitoxin

• AlphaFold DB: A0A0H3HDW8