Detailed information of TA system TA022818

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 3621646..3622192
• Replicon: chromosome
• Accession: NC_010581
• Organism: Beijerinckia indica subsp. indica ATCC 9039

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: B2ICD9
• Locus tag: BIND_RS16090
• Protein ID: WP_012386084.1
• Coordinates: 3621935..3622192 (-)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: B2ICD8
• Locus tag: BIND_RS16085
• Protein ID: WP_012386083.1
• Coordinates: 3621646..3621951 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BIND_RS16050	3617404..3617781	+	378	WP_012386079.1	phage tail assembly protein	-
BIND_RS16065	3618695..3620110	+	1416	WP_081433793.1	phage tail tape measure protein	-
BIND_RS21810	3620114..3620512	+	399	WP_012386080.1	hypothetical protein	-
BIND_RS16075	3620718..3621200	+	483	WP_012386081.1	hypothetical protein	-
BIND_RS16080	3621294..3621500	-	207	WP_202944796.1	helix-turn-helix domain-containing protein	-
BIND_RS16085	3621646..3621951	-	306	WP_012386083.1	BrnA antitoxin family protein	Antitoxin
BIND_RS16090	3621935..3622192	-	258	WP_012386084.1	BrnT family toxin	Toxin
BIND_RS16095	3622541..3622978	-	438	WP_012386085.1	DUF2335 domain-containing protein	-
BIND_RS20270	3623757..3624821	-	1065	WP_012386088.1	hypothetical protein	-
BIND_RS21675	3624931..3625104	+	174	WP_158304397.1	hypothetical protein	-
BIND_RS16120	3625485..3626039	-	555	WP_012386089.1	hypothetical protein	-
BIND_RS16125	3626842..3627063	-	222	Protein_3159	helix-turn-helix transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-77)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (27-96)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 9717.18 Da
• Isoelectric Point: 5.0790

>T22818 WP_012386084.1 NC_010581:c3622192-3621935 [Beijerinckia indica subsp. indica ATCC 9039]
MEILWDEPKRQNTLKTRGMDFANVTEEFFEAALIVQAKNNRFMAIGIIDDEPISVVFLPLGTEAISIISMRHASRKERNA
YAQAF

Nucleotide

• Length: 258 bp

>T22818 NC_010581:c3622192-3621935 [Beijerinckia indica subsp. indica ATCC 9039]
ATGGAAATTCTCTGGGACGAGCCGAAGCGCCAAAACACTCTCAAGACGAGAGGAATGGACTTCGCGAACGTGACGGAGGA
ATTCTTCGAAGCCGCCCTGATCGTCCAGGCCAAGAACAATCGCTTCATGGCGATTGGAATAATCGACGACGAGCCAATTT
CAGTTGTCTTCCTGCCGCTCGGCACCGAGGCCATTTCGATCATCAGCATGCGTCATGCTAGCCGCAAGGAAAGGAACGCC
TATGCCCAAGCATTCTAG

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11591.09 Da
• Isoelectric Point: 7.2081

>AT22818 WP_012386083.1 NC_010581:c3621951-3621646 [Beijerinckia indica subsp. indica ATCC 9039]
MPKHSRIRRLGDAEEAKIQDQIASDPDDFEPTDEELANARPFAEVFPHLAETIRRRGPLKKKEAVSIRIDIDVLQKLRAT
GSGWQSRVNDVLREFVEGKQR

Nucleotide

• Length: 306 bp

>AT22818 NC_010581:c3621951-3621646 [Beijerinckia indica subsp. indica ATCC 9039]
ATGCCCAAGCATTCTAGGATTCGGCGCCTTGGTGATGCCGAGGAAGCAAAGATTCAGGACCAAATTGCCTCCGATCCTGA
TGATTTTGAACCAACAGATGAGGAATTGGCTAATGCCCGTCCCTTTGCTGAGGTATTCCCGCATCTTGCGGAAACAATCC
GCCGACGTGGCCCTCTAAAGAAAAAAGAAGCGGTCTCAATACGGATCGATATTGATGTCCTGCAAAAGCTCCGCGCGACC
GGAAGCGGCTGGCAATCGCGCGTGAATGATGTTCTTAGAGAGTTTGTCGAGGGAAAACAGAGGTAA

Structures

Toxin

• AlphaFold DB: B2ICD9

Antitoxin

• AlphaFold DB: B2ICD8