Detailed information of TA system TA228179

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/ElaA-DUF1778
• Location: 8379..9130
• Replicon: plasmid pKOX205_1
• Accession: NZ_CP089404
• Organism: Klebsiella pasteurii strain Kox205

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: A0A071LQ79
• Locus tag: LUW96_RS28370
• Protein ID: WP_036114515.1
• Coordinates: 8648..9130 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: -
• Locus tag: LUW96_RS28365
• Protein ID: WP_049095199.1
• Coordinates: 8379..8657 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LUW96_RS28335 (LUW96_28335)	3790..4080	+	291	WP_032413253.1	MSMEG_0570 family nitrogen starvation response protein	-
LUW96_RS28340 (LUW96_28340)	4098..5363	+	1266	WP_048281137.1	MSMEG_0569 family flavin-dependent oxidoreductase	-
LUW96_RS28345 (LUW96_28345)	5344..7014	+	1671	WP_032413257.1	AMP-binding protein	-
LUW96_RS28350 (LUW96_28350)	7098..7208	-	111	Protein_8	glutathione ABC transporter permease GsiC	-
LUW96_RS30085	7243..7389	+	147	Protein_9	DUF2235 domain-containing protein	-
LUW96_RS28360 (LUW96_28360)	8045..8257	+	213	WP_049095200.1	hypothetical protein	-
LUW96_RS28365 (LUW96_28365)	8379..8657	+	279	WP_049095199.1	DUF1778 domain-containing protein	Antitoxin
LUW96_RS28370 (LUW96_28370)	8648..9130	+	483	WP_036114515.1	GNAT family N-acetyltransferase	Toxin
LUW96_RS28375 (LUW96_28375)	9263..10187	-	925	Protein_13	IS5 family transposase	-
LUW96_RS28380 (LUW96_28380)	10181..10606	+	426	WP_227543254.1	hypothetical protein	-
LUW96_RS28385 (LUW96_28385)	10765..11127	+	363	WP_117389542.1	type II toxin-antitoxin system VapC family toxin	-
LUW96_RS28390 (LUW96_28390)	11219..12622	-	1404	WP_117389535.1	glycoside hydrolase family 1 protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	pla	1..149463	149463
	-	flank	IS/Tn	-	-	9263..10078	815

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-146)

Antitoxin

• Vibrio phage ICP1, Orf50 (3-92)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17694.45 Da
• Isoelectric Point: 8.9130

>T228179 WP_036114515.1 NZ_CP089404:8648-9130 [Klebsiella pasteurii]
MGMRAPESLTSEHNIAEFCCQEPALNEWLKKKALRNHSTGISRVYVVCAENTNRVIGYYCLSSGSVHRNTVPGAYRRNAP
DVIPVIVLGRLAIDQAWSGNGLGAALLKDAIYRTQNIAFQVGVRALAVHALNEEVKCFYTRFGFEPSIVNTLTLLFPIKV

Nucleotide

• Length: 483 bp

>T228179 NZ_CP123430:c5028719-5028336 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10734.25 Da
• Isoelectric Point: 4.5271

>AT228179 WP_049095199.1 NZ_CP089404:8379-8657 [Klebsiella pasteurii]
MKPDVREAPINIRAKTSQRDLIDMAASLVSKSRTDFMLEVACREAQDILLDQRLFLLNDNQFETFIEELDAPITPERQAR
IDNLMNRKSPWE

Nucleotide

• Length: 279 bp

>AT228179 NZ_CP123430:c5028961-5028719 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Structures

Toxin

• AlphaFold DB: A0A071LQ79