Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 2824756..2825375 Replicon chromosome
Accession NZ_CP089403
Organism Klebsiella pasteurii strain Kox205

Toxin (Protein)


Gene name Hha Uniprot ID H3N9D8
Locus tag LUW96_RS13305 Protein ID WP_004099646.1
Coordinates 2824756..2824974 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID H3N7X7
Locus tag LUW96_RS13310 Protein ID WP_004129911.1
Coordinates 2825001..2825375 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LUW96_RS13270 (LUW96_13270) 2820804..2821067 + 264 WP_049114567.1 type B 50S ribosomal protein L31 -
LUW96_RS13275 (LUW96_13275) 2821067..2821207 + 141 WP_003859006.1 type B 50S ribosomal protein L36 -
LUW96_RS13280 (LUW96_13280) 2821204..2821902 - 699 WP_049114564.1 GNAT family protein -
LUW96_RS13285 (LUW96_13285) 2822002..2823456 + 1455 WP_232391352.1 PLP-dependent aminotransferase family protein -
LUW96_RS13290 (LUW96_13290) 2823431..2823901 - 471 WP_004129906.1 YlaC family protein -
LUW96_RS13295 (LUW96_13295) 2823922..2824056 + 135 WP_224326009.1 hypothetical protein -
LUW96_RS13300 (LUW96_13300) 2824028..2824594 - 567 WP_049114562.1 maltose O-acetyltransferase -
LUW96_RS13305 (LUW96_13305) 2824756..2824974 - 219 WP_004099646.1 HHA domain-containing protein Toxin
LUW96_RS13310 (LUW96_13310) 2825001..2825375 - 375 WP_004129911.1 Hha toxicity modulator TomB Antitoxin
LUW96_RS13315 (LUW96_13315) 2825843..2828989 - 3147 WP_004129913.1 multidrug efflux RND transporter permease subunit AcrB -
LUW96_RS13320 (LUW96_13320) 2829012..2830205 - 1194 WP_004129915.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8640.05 Da        Isoelectric Point: 8.9008

>T228170 WP_004099646.1 NZ_CP089403:c2824974-2824756 [Klebsiella pasteurii]
MSDKTLTKIDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPPSVWKFIR

Download         Length: 219 bp

>T228170 NZ_CP123403:c1010737-1010423 [Trueperella pyogenes]
GTGAAGATTCGGCAAACCGACGTGTATGCTCACTGGTTTCGCGAGCTAAAAGATGTCCAGAGCAAGGCCCGAATCAATAT
TGCACTTCAGCGTTGCAAAATAGCTGGCGAAGTAGTGGGTGACGTCAAGCCGGTTGGTGATGGCGTTTTTGAGATGCGAA
TTCACGTTGGCCCCGGCTATCGGATTTACTACATGTCAAGAGGGAACCAACTTATGCTACTAGTCATTGGTGGTGATAAG
TCCACCCAGCAGCGTGACATAACCAAAGCTAAACAGCTTGCAACAGAAATTATTAGGGAGGGATCATGGGAGTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14350.09 Da        Isoelectric Point: 4.8989

>AT228170 WP_004129911.1 NZ_CP089403:c2825375-2825001 [Klebsiella pasteurii]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIAAFALNYKIKYAEDNKLITQIDEYL
DDTFVLFSNYGINSADLQKWRKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT228170 NZ_CP123403:c1010432-1010139 [Trueperella pyogenes]
ATGGGAGTAAAAATCAGCACCTGGGATGCCAGCGAATACCTTGAAACCAAGGAAGATATTGCTGCCTACCTGAACGCGGC
ACTCGAAGAGGGCGACCCGGCTCTCCTGCAGGTCGCACTTGGTGATGTCGCCAAAGCGCGTGGTGTGACCGCTATCGCGC
GAGAAGCCGGTGTTGGACGCGAGTCTCTCTACAAGTCGCTTTCCGAAAACGGCAATCCGTCCTGGCGGACCATCACAAAG
GTTCTCACTGCACTCGGGCTGAAGATCGAAGTCAAAGCCATTGCGTTAGCGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3H713

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3HD25

References