Detailed information of TA system TA228166

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 76157..76893
• Replicon: plasmid pKOX58_1
• Accession: NZ_CP089396
• Organism: Klebsiella michiganensis strain Kox58

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: A0A2J5Q928
• Locus tag: LUW95_RS29155
• Protein ID: WP_004098919.1
• Coordinates: 76411..76893 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: A0A2J5Q9C0
• Locus tag: LUW95_RS29150
• Protein ID: WP_004098921.1
• Coordinates: 76157..76423 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LUW95_RS29120 (LUW95_29120)	72077..72757	+	681	WP_004098938.1	copper response regulator transcription factor PcoR	-
LUW95_RS29125 (LUW95_29125)	72754..74154	+	1401	WP_004098935.1	copper resistance membrane spanning protein PcoS	-
LUW95_RS29130 (LUW95_29130)	74370..74804	+	435	WP_004098933.1	hypothetical protein	-
LUW95_RS29135 (LUW95_29135)	75142..75345	+	204	WP_004098931.1	HHA domain-containing protein	-
LUW95_RS29140 (LUW95_29140)	75394..75651	+	258	WP_004098928.1	hypothetical protein	-
LUW95_RS29145 (LUW95_29145)	75727..75981	+	255	WP_004098924.1	hypothetical protein	-
LUW95_RS29150 (LUW95_29150)	76157..76423	+	267	WP_004098921.1	DUF1778 domain-containing protein	Antitoxin
LUW95_RS29155 (LUW95_29155)	76411..76893	+	483	WP_004098919.1	GNAT family N-acetyltransferase	Toxin
LUW95_RS29160 (LUW95_29160)	77102..78448	+	1347	WP_077257948.1	ISNCY family transposase	-
LUW95_RS29165 (LUW95_29165)	78506..79309	+	804	WP_064407410.1	tetratricopeptide repeat protein	-
LUW95_RS29170 (LUW95_29170)	79323..80684	+	1362	WP_059509657.1	FRG domain-containing protein	-
LUW95_RS29175 (LUW95_29175)	80916..81356	+	441	WP_059509659.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaSHV-12	-	1..136868	136868

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (66-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17335.06 Da
• Isoelectric Point: 10.0704

>T228166 WP_004098919.1 NZ_CP089396:76411-76893 [Klebsiella michiganensis]
VGRITAPEPLSSAHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKKGTKKVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Nucleotide

• Length: 483 bp

>T228166 NZ_CP123381:1266463-1266867 [Gordonia sp. SMJS1]
TTGATCTATCTCGACACCGCAGCGATGGTGAAACTCGTGGTGCGCGAGGCCGAGACCGCTCAGCTGATCGAGTGGCTCGC
GCTGCATCCAGGGGTCCCGACCGTGACGAGTGCGCTGGGACGAGTCGAATTGATGCGCACCGCGTTGCGTGATGGCAGCC
CGGGCCTTCATGAGCGAGCGCGTGTTCTCCTCGATGCTCTGGACGTGATCCCGCTGAGCGTTACCGTCATCGACATCGCC
GAATCGATCGGCCCGTCTTCGCTGCGCTCGCTCGACGCCTTGCATCTCGCCTCTGCGAGCGTCATACGCGCCGAGATGAG
TGCCTTCGTCGCTTATGATCACCGGCTTCTGGCGGGCGCATCGACGCTTGGCTACCCGACCGTTGCTCCCGGCGCCGAAT
CGTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10105.49 Da
• Isoelectric Point: 4.5141

>AT228166 WP_004098921.1 NZ_CP089396:76157-76423 [Klebsiella michiganensis]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEIACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDDSAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT228166 NZ_CP123381:1266194-1266466 [Gordonia sp. SMJS1]
ATGAGGACGATCGGTATCCGCGAGCTCAGGCAGCACGCCTCGCGGTATCTCGCGCAGGTCGAGGCCGGGGAAGAGTTGGC
CATCACCAACCGAGGCCGCACGGTTGCGCGGTTGGTCCCGGTGTCTGCGGGGGAGAGAAGTAGAGACGCGCTCATCGAGG
CCGGGATACTCCTGCCGGCCCGACGTGCGGGCGGCATAACCGGGCTGCACCCCGAGACGTTGCCGGCGCGCGCGCTCACC
GAAGTCCTCGACCAGGTTCGCGAGGATCGTTGA

Structures

Toxin

• AlphaFold DB: A0A2J5Q928

Antitoxin

• AlphaFold DB: A0A2J5Q9C0