Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 5800639..5801186 Replicon chromosome
Accession NZ_CP089395
Organism Klebsiella michiganensis strain Kox58

Toxin (Protein)


Gene name ccdB Uniprot ID A0A0H3H5T3
Locus tag LUW95_RS27490 Protein ID WP_014227299.1
Coordinates 5800878..5801186 (+) Length 103 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID A0A0H3H0P7
Locus tag LUW95_RS27485 Protein ID WP_014227298.1
Coordinates 5800639..5800875 (+) Length 79 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LUW95_RS27470 (LUW95_27470) 5796846..5798012 + 1167 WP_014227296.1 multidrug effflux MFS transporter -
LUW95_RS27475 (LUW95_27475) 5798173..5799075 + 903 WP_232421357.1 N-acetylmuramic acid 6-phosphate etherase -
LUW95_RS27480 (LUW95_27480) 5799100..5800533 + 1434 WP_014839829.1 PTS N-acetylmuramic acid transporter subunit IIBC -
LUW95_RS27485 (LUW95_27485) 5800639..5800875 + 237 WP_014227298.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
LUW95_RS27490 (LUW95_27490) 5800878..5801186 + 309 WP_014227299.1 CcdB family protein Toxin
LUW95_RS27495 (LUW95_27495) 5801337..5802239 + 903 WP_232421358.1 EamA family transporter -
LUW95_RS27500 (LUW95_27500) 5802243..5802500 - 258 WP_032718834.1 hypothetical protein -
LUW95_RS27505 (LUW95_27505) 5802500..5803477 - 978 WP_032695133.1 Rpn family recombination-promoting nuclease/putative transposase -
LUW95_RS27510 (LUW95_27510) 5803687..5803980 + 294 WP_232421359.1 YicS family protein -
LUW95_RS27515 (LUW95_27515) 5804004..5804945 - 942 WP_014227303.1 LacI family DNA-binding transcriptional regulator -
LUW95_RS27520 (LUW95_27520) 5805240..5805551 + 312 WP_014839834.1 PTS sugar transporter subunit IIB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-101)

Antitoxin

Post-segregation antitoxin CcdA

(8-78)


Sequences

Toxin        


Download         Length: 103 a.a.        Molecular weight: 11725.57 Da        Isoelectric Point: 7.2729

>T228164 WP_014227299.1 NZ_CP089395:5800878-5801186 [Klebsiella michiganensis]
MQYYVYKNTGRIAAYPYLLDVQSDIIGKRNTRVVIPLFPLKNYKGPRADRLTPLVTVEGEEYVVMTHELASIPHRVLGEE
VCNLNHQREVVKASMDFLFDGI

Download         Length: 309 bp

>T228164 NZ_CP123373:c17180-16905 [Providencia rettgeri]
ATGCTAACTCCTATTCGTTCTAGTGCTTTTAAGGCGGATGTTAAGCGACAAAAAAAAAGAGGGAAGGATTTAAACAAGCT
AAAAGTATTAATTGAGTTACTAATAAAGGAAGAACAGATCCCTGCGGACTATGTAGACCACCCTTTAACAGGTAATTGGC
GAGGATATAGAGATGCTCATATTGAAGGCGATTGGCTACTAATTTATAAAATTACTGGTCAAGAATTAAAGTTAGCGAGA
ACGGGTTCTCACCAAGATATTTTTAGCCAATATTAA

Antitoxin


Download         Length: 79 a.a.        Molecular weight: 8783.92 Da        Isoelectric Point: 5.7928

>AT228164 WP_014227298.1 NZ_CP089395:5800639-5800875 [Klebsiella michiganensis]
MPAAKRNKQNVSVTLDPGLVKQAREVGINLSLTLTNALKSEIHSLEIARWQKENAQAIDYLNNLTEENGLLSDEYRTF

Download         Length: 237 bp

>AT228164 NZ_CP123373:c17437-17174 [Providencia rettgeri]
ATGATTTCTGATACGACTGTTAAAGCTAGAATTTCTCAAGATGTCAAAGAACGAGCTGTATCTGCACTTGCAAAGATGGG
CTTAAATACATCTGATCTTATCCGTATGGTTATGATTCGTGTTGCCGATGAAGGAAAACTACCATTTGATATCAAAACAC
CAAATGTTGTGACTAAAGAGGCAATTGAAGAATTAGAAAAAGGGAATGGAAAAGCATTTTCTTCAACCAATGCACTTTTC
GATGATTTGGGGATCTGATGCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3H5T3

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3H0P7

References