Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 4970030..4970687 Replicon chromosome
Accession NZ_CP089395
Organism Klebsiella michiganensis strain Kox58

Toxin (Protein)


Gene name cptA Uniprot ID J5U333
Locus tag LUW95_RS23450 Protein ID WP_004854060.1
Coordinates 4970030..4970440 (-) Length 137 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID H3N295
Locus tag LUW95_RS23455 Protein ID WP_004124953.1
Coordinates 4970421..4970687 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LUW95_RS23430 (LUW95_23430) 4966007..4967740 - 1734 WP_032693806.1 single-stranded-DNA-specific exonuclease RecJ -
LUW95_RS23435 (LUW95_23435) 4967746..4968459 - 714 WP_004854054.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
LUW95_RS23440 (LUW95_23440) 4968482..4969378 - 897 WP_004105555.1 site-specific tyrosine recombinase XerD -
LUW95_RS23445 (LUW95_23445) 4969500..4970021 + 522 WP_014226792.1 flavodoxin FldB -
LUW95_RS23450 (LUW95_23450) 4970030..4970440 - 411 WP_004854060.1 protein YgfX Toxin
LUW95_RS23455 (LUW95_23455) 4970421..4970687 - 267 WP_004124953.1 FAD assembly factor SdhE Antitoxin
LUW95_RS23460 (LUW95_23460) 4970932..4971915 + 984 WP_032720095.1 tRNA-modifying protein YgfZ -
LUW95_RS23465 (LUW95_23465) 4972109..4974907 + 2799 WP_025105970.1 transcriptional regulator DagR -
LUW95_RS23470 (LUW95_23470) 4975028..4975453 + 426 WP_032720093.1 PTS sugar transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 137 a.a.        Molecular weight: 16106.97 Da        Isoelectric Point: 10.9455

>T228163 WP_004854060.1 NZ_CP089395:c4970440-4970030 [Klebsiella michiganensis]
VVLWQSDLRISWRAQWFSLLMHGVVAALVLLMPWPLSYTPLWLILLSLVVFDCVRSQRRIHARQGEIKLLIDSRLRWQKA
EWDIIGTPWVINSGMLLRLRNTENQRTQHLWVAADSMDAGEWRDLRRLVLLKPTQE

Download         Length: 411 bp

>T228163 NZ_CP123369:85467-85742 [Providencia rettgeri]
ATGCTAACTCCTATTCGTTCTAGTGCTTTTAAGGCGGATGTTAAGCGACAAAAAAAAAGAGGGAAGGATTTAAACAAGCT
AAAAGTATTAATTGAGTTACTAATAAAGGAAGAACAGATCCCTGCGGACTATGTAGACCACCCTTTAACAGGTAATTGGC
GAGGATATAGAGATGCTCATATTGAAGGCGATTGGCTACTAATTTATAAAATTACTGGTCAAGAATTAAAGTTAGCGAGA
ACGGGTTCTCACCAAGATATTTTTAGCCAATATTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10411.88 Da        Isoelectric Point: 5.1296

>AT228163 WP_004124953.1 NZ_CP089395:c4970687-4970421 [Klebsiella michiganensis]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDTLSDGDKALFIRLLENDDPDLFNWLMNHGEPADAELQRMVKLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT228163 NZ_CP123369:85210-85473 [Providencia rettgeri]
ATGATTTCTGATACGACTGTTAAAGCTAGAATTTCTCAAGATGTCAAAGAACGAGCTGTATCTGCACTTGCAAAGATGGG
CTTAAATACATCTGATCTTATCCGTATGGTTATGATTCGTGTTGCCGATGAAGGAAAACTACCATTTGATATCAAAACAC
CAAATGTTGTGACTAAAGAGGCAATTGAAGAATTAGAAAAAGGGAATGGAAAAGCATTTTCTTCAACCAATGCACTTTTC
GATGATTTGGGGATCTGATGCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYA5

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3H3L9

References