Detailed information of TA system TA228162

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 3776879..3777469
• Replicon: chromosome
• Accession: NZ_CP089395
• Organism: Klebsiella michiganensis strain Kox58

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: LUW95_RS17875
• Protein ID: WP_032720306.1
• Coordinates: 3776879..3777211 (-)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: J6I3K7
• Locus tag: LUW95_RS17880
• Protein ID: WP_004852307.1
• Coordinates: 3777212..3777469 (-)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LUW95_RS17850 (LUW95_17850)	3772290..3772547	+	258	WP_014229908.1	histidine kinase	-
LUW95_RS17855 (LUW95_17855)	3772680..3774134	+	1455	WP_014229909.1	AMP nucleosidase	-
LUW95_RS17865 (LUW95_17865)	3775099..3776556	-	1458	WP_014229916.1	EmmdR/YeeO family multidrug/toxin efflux MATE transporter	-
LUW95_RS17875 (LUW95_17875)	3776879..3777211	-	333	WP_032720306.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
LUW95_RS17880 (LUW95_17880)	3777212..3777469	-	258	WP_004852307.1	antitoxin	Antitoxin
LUW95_RS17885 (LUW95_17885)	3778475..3779389	-	915	WP_032720305.1	LysR family transcriptional regulator	-
LUW95_RS17890 (LUW95_17890)	3779490..3780488	+	999	WP_232421174.1	aldo/keto reductase	-
LUW95_RS17895 (LUW95_17895)	3780526..3781452	-	927	WP_014838892.1	LysR substrate-binding domain-containing protein	-
LUW95_RS17900 (LUW95_17900)	3781632..3782237	+	606	WP_014838893.1	glutathione S-transferase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-109)

Antitoxin

• Antitoxin PemI-like (4-84)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11831.77 Da
• Isoelectric Point: 10.5834

>T228162 WP_032720306.1 NZ_CP089395:c3777211-3776879 [Klebsiella michiganensis]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPITSGGNFARTAGFTVPLEGAGIKTTGVIRCDQPRTI
DMAARNGKRLERIPDAVVNEVLARLDAILS

Nucleotide

• Length: 333 bp

>T228162 NZ_CP123369:c17598-17266 [Providencia rettgeri]
ATGGTATCTCGCTACGTGCCTGATTCTGGCGATTTGATTTGGATTGACTTTGATCCAGTTGAAGGACATGAGCAGGGCGG
TCATCGTCCAGCAGTTGTCCTCAGCCCATTTGCATACAATAATAAAGTAGGACTCTTATTATGCGTACCATGTACAACTA
AGGGCAAAGGCTATCCATTTGAATGCGAACTCTCGAACGAAAGAGACAGCTACGCATTAGCGGATCAAGTTACATGTGTT
GACTGGAGAGCTCGTAATGTTACTAAAAAGGGCAAAGTGGAGGCATCAGAACTGGCAGAGATAAAAGCTAAAGCAAAGGC
GTTAATAGGTTAG

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9422.88 Da
• Isoelectric Point: 4.4607

>AT228162 WP_004852307.1 NZ_CP089395:c3777469-3777212 [Klebsiella michiganensis]
MYTTRLKKVGGSIMLAVPPAVLKTLELSTDSEVGMTINNGCLIIEPQKRPHYSLEELLAQCDPHAEMSEEDREWIDAPAV
GKEIL

Nucleotide

• Length: 258 bp

>AT228162 NZ_CP123369:c17843-17598 [Providencia rettgeri]
ATGGCTCAGGTCACAGTAAAAAAATGGGGCAATAGCCCGTCTGTACGGCTTCCTGTTGCAATCATGCAAAAAGCGGCACT
TAGCGTTGATGATACGGTTGAAATAGCGGTAGAAGAAGGTCGTATCATCATTACGCCAGTTAAAGCGGTAGAATACTCAT
TAGAAAATTTGTTAGCCGGTATCACGCCGGAGAATATTCATGAGAAAGTAGATTTCGGAGCTCGCACAGGTAAGGAATTA
ATATAA

Structures

Antitoxin

• AlphaFold DB: A0A0H3HDW8