228153

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1237743..1238362 Replicon chromosome
Accession NZ_CP089395
Organism Klebsiella michiganensis strain Kox58

Toxin (Protein)


Gene name Hha Uniprot ID H3N9D8
Locus tag LUW95_RS05745 Protein ID WP_004099646.1
Coordinates 1237743..1237961 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID H3N7X7
Locus tag LUW95_RS05750 Protein ID WP_004129911.1
Coordinates 1237988..1238362 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LUW95_RS05710 (LUW95_05710) 1233788..1234051 + 264 WP_004848323.1 type B 50S ribosomal protein L31 -
LUW95_RS05715 (LUW95_05715) 1234051..1234191 + 141 WP_003859006.1 type B 50S ribosomal protein L36 -
LUW95_RS05720 (LUW95_05720) 1234188..1234886 - 699 WP_220981614.1 GNAT family protein -
LUW95_RS05725 (LUW95_05725) 1234988..1236442 + 1455 WP_232421725.1 PLP-dependent aminotransferase family protein -
LUW95_RS05730 (LUW95_05730) 1236417..1236887 - 471 WP_014228205.1 YlaC family protein -
LUW95_RS05735 (LUW95_05735) 1236908..1237042 + 135 WP_223226764.1 hypothetical protein -
LUW95_RS05740 (LUW95_05740) 1237014..1237580 - 567 WP_038424164.1 maltose O-acetyltransferase -
LUW95_RS05745 (LUW95_05745) 1237743..1237961 - 219 WP_004099646.1 HHA domain-containing protein Toxin
LUW95_RS05750 (LUW95_05750) 1237988..1238362 - 375 WP_004129911.1 Hha toxicity modulator TomB Antitoxin
LUW95_RS05755 (LUW95_05755) 1238843..1241989 - 3147 WP_014228207.1 multidrug efflux RND transporter permease subunit AcrB -
LUW95_RS05760 (LUW95_05760) 1242012..1243205 - 1194 WP_042944463.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8640.05 Da        Isoelectric Point: 8.9008

>T228153 WP_004099646.1 NZ_CP089395:c1237961-1237743 [Klebsiella michiganensis]
MSDKTLTKIDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPPSVWKFIR

Download         Length: 219 bp

>T228153 NZ_CP123365:c3669700-3669593 [Shigella flexneri]
ATGACGCTCGCAGAACTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAATAAGCGGAAGTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14350.09 Da        Isoelectric Point: 4.8989

>AT228153 WP_004129911.1 NZ_CP089395:c1238362-1237988 [Klebsiella michiganensis]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIAAFALNYKIKYAEDNKLITQIDEYL
DDTFVLFSNYGINSADLQKWRKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT228153 NZ_CP123365:3669757-3669812 [Shigella flexneri]
TCAAGATTAGCCCCCGTGATGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3H713

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3HD25

References