Detailed information of TA system TA228152

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yjhX-yjhQ/YjhX(toxin)
• Location: 1008048..1008867
• Replicon: chromosome
• Accession: NZ_CP089395
• Organism: Klebsiella michiganensis strain Kox58

Toxin (Protein)

• Gene name: yjhX
• Uniprot ID: J5WT09
• Locus tag: LUW95_RS04650
• Protein ID: WP_004110819.1
• Coordinates: 1008048..1008305 (+)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: yjhQ
• Uniprot ID: -
• Locus tag: LUW95_RS04655
• Protein ID: WP_201208115.1
• Coordinates: 1008316..1008867 (+)
• Length: 184 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LUW95_RS04635 (LUW95_04635)	1003724..1004500	-	777	WP_004847986.1	Uxu operon transcriptional regulator	-
LUW95_RS04640 (LUW95_04640)	1004639..1006114	-	1476	WP_014228053.1	fructuronate reductase	-
LUW95_RS04645 (LUW95_04645)	1006188..1007372	-	1185	WP_014228054.1	mannonate dehydratase	-
LUW95_RS04650 (LUW95_04650)	1008048..1008305	+	258	WP_004110819.1	YjhX family toxin	Toxin
LUW95_RS04655 (LUW95_04655)	1008316..1008867	+	552	WP_201208115.1	N-acetyltransferase	Antitoxin
LUW95_RS04660 (LUW95_04660)	1008981..1009391	+	411	Protein_888	class I SAM-dependent methyltransferase	-
LUW95_RS04665 (LUW95_04665)	1009767..1010513	+	747	WP_014228056.1	class I SAM-dependent methyltransferase	-
LUW95_RS04670 (LUW95_04670)	1010728..1011615	-	888	WP_232421473.1	DMT family transporter	-
LUW95_RS04675 (LUW95_04675)	1011735..1012643	+	909	WP_014228058.1	transcriptional regulator GcvA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Putative toxin of bacterial toxin-antitoxin pair (1-85)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 9552.07 Da
• Isoelectric Point: 11.1381

>T228152 WP_004110819.1 NZ_CP089395:1008048-1008305 [Klebsiella michiganensis]
MNLSRQEQRTLHVLAKGGRIAHIRDASGRVTSVECYSREGLLLSDCTLAVFKKLKTKKLIKSVNGQPYRINTTGLNNVRA
QLDNR

Nucleotide

• Length: 258 bp

>T228152 NZ_CP123365:c3590263-3589988 [Shigella flexneri]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGCAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
TGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin

Protein

• Length: 184 a.a.
• Molecular weight: 20048.84 Da
• Isoelectric Point: 6.3282

>AT228152 WP_201208115.1 NZ_CP089395:1008316-1008867 [Klebsiella michiganensis]
MTNHSFTFHVASEGDTDDILDVETRAFGYSKEARLVADLLNDESACPTLSLLARHNGEAVGHILFTRATFKGEPDSPMMH
ILAPLAVVPEYQGVGVGGELIRHGIEQLKAMGSQMVFVLGHAAYYPRHGFEPGAGDKGYPAPYPIPEAHKACWMLQPISS
QPLGRTGQIQCARALMKPEHWRE

Nucleotide

• Length: 552 bp

>AT228152 NZ_CP123365:c3589991-3589632 [Shigella flexneri]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCAGCTGTTATTACCTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGTATTCAGGGGCTGCAAAAAGAAGGAG
AGCTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Structures

Toxin

• AlphaFold DB: A0A0H3H6Y2