Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 53536..54272 Replicon plasmid pKPN692_1
Accession NZ_CP089386
Organism Klebsiella variicola subsp. variicola strain KPN692

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag LUX40_RS26635 Protein ID WP_003026803.1
Coordinates 53790..54272 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag LUX40_RS26630 Protein ID WP_003026799.1
Coordinates 53536..53802 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LUX40_RS26585 (LUX40_26585) 49736..50098 - 363 WP_004152100.1 arsenite efflux transporter metallochaperone ArsD -
LUX40_RS26590 (LUX40_26590) 50148..50498 - 351 WP_004152101.1 As(III)-sensing metalloregulatory transcriptional repressor ArsR -
LUX40_RS26595 (LUX40_26595) 50856..51104 + 249 WP_019725036.1 hypothetical protein -
LUX40_RS26600 (LUX40_26600) 51101..51673 + 573 WP_015065504.1 hypothetical protein -
LUX40_RS26605 (LUX40_26605) 51704..52198 + 495 WP_009310053.1 hypothetical protein -
LUX40_RS26610 (LUX40_26610) 52242..52610 + 369 WP_015065502.1 hypothetical protein -
LUX40_RS26615 (LUX40_26615) 52644..52847 + 204 WP_019725040.1 HHA domain-containing protein -
LUX40_RS26620 (LUX40_26620) 52861..53091 + 231 WP_019725034.1 hypothetical protein -
LUX40_RS26625 (LUX40_26625) 53135..53329 - 195 WP_019725033.1 hypothetical protein -
LUX40_RS26630 (LUX40_26630) 53536..53802 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
LUX40_RS26635 (LUX40_26635) 53790..54272 + 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
LUX40_RS26640 (LUX40_26640) 54480..55826 + 1347 WP_077253535.1 ISNCY family transposase -
LUX40_RS26645 (LUX40_26645) 55875..56270 + 396 WP_004143398.1 helix-turn-helix domain-containing protein -
LUX40_RS26650 (LUX40_26650) 56418..57583 - 1166 Protein_59 IS3 family transposase -
LUX40_RS26655 (LUX40_26655) 57760..58722 - 963 WP_004152113.1 zinc metalloprotease HtpX -
LUX40_RS26660 (LUX40_26660) 58709..59197 - 489 WP_004152114.1 phosphate-starvation-inducible PsiE family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sul2 / aph(3'')-Ib / aph(6)-Id / blaTEM-1B / blaCTX-M-15 / dfrA14 / qnrB1 / tet(A) / aac(6')-Ib-cr - 1..289376 289376


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T228150 WP_003026803.1 NZ_CP089386:53790-54272 [Klebsiella variicola subsp. variicola]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T228150 NZ_CP123365:3097113-3097490 [Shigella flexneri]
ATGAATACATTACCCGACACTCACGTACGGGAGGCATCGGGCTGCCCGTCTCCCATCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGAATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGCCGGGATTC
AGCGCAGGAGCCCCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCACGCCGGGCAACCGGCCTGATGACCCGCGA
TAACTACAGAACGGTAAATAACATTACCCGGGGGAAGCATCCGGAAGCGAAACAATGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT228150 WP_003026799.1 NZ_CP089386:53536-53802 [Klebsiella variicola subsp. variicola]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT228150 NZ_CP123365:3096689-3097066 [Shigella flexneri]
GTGTCAGACACACTCCCCGGGACAACGCCTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCCCGACTGGTACAGGAGGGTAACCGCCTGCATTACCTTGCAGACCGCGCCGGTATCAGAGGCAGGT
TCAGCGACGTAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACTGGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACACTGTATGCGAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCGGCAGCGCCCGCAACCACCGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References